Colorimetric ATPase Assay Kit (601-0121)
Colorimetric ATPase Assay Kit 480Tests
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Colorimetric ATPase Assay Kit 480Tests Summary: | | | | Specificity: | Innova's high sensitivity colorimetric ATPase assay system includes:
Specially purified, Pi-free ATP
PiColorlock Gold (superior malachite formulation)*
Stabiliser to prevent high backgrounds from non-enzymatic Pi release
High sensitivity (OD 1 for 5% substrate hydrolysis)
No precipitation of dye-Pi complexes
Simple protocol
Supplied with Pi standard and 96-well microplates |
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| Publications: | Antibody has been mentioned in at least 5 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Colorimetric ATPase Assay Products | Unit Size: | 480 Tests |
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| Concentration: | Concentration is not relevant for this product. Please see the protocols for proper use of this product. |
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Packaging: | Storage: | Storage is content dependent. |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: This non radioactive colorimetric assay kit uses a 96 well format, and all the necessary reagents are supplied for measuring enzyme activity and are ideal for high throughput drug screening.
Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions. Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate.
At the heart of our assay kits is PiColorLock Gold, a superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes. Together with specially purified substrates and proprietary stabilizers our kits offer the lowest possible assay backgrounds and outstanding assay performance.
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Publications (5)Colorimetric ATPase Assay Kit 480Tests Product Specific:- Hare S, Fischer W, Williams R, et al. Identification, Structure and Mode of Action of a New Regulator of the Helicobacter Pylori HP0525 ATPase. EMBO Journal (2007), 26 pp 4926-4934. [PMID: 17972918]
- Consumption of Soy Protein Isolate Modulates the Phosphorylation Status of Hepatic ATPase/ATP Synthase ß Protein and Increases ATPase Activity in Rats. The Journal of Nutrition (2007), 137 pp 2029-2035.
more... - Stauff DL, Bagaley D, Torres VJ, et al. Staphylococcus Aureus HrtA Is an ATPase Required for Protection Against Heme Toxicity and Prevention of a Transcriptional Heme Stress Response Journal of Bacteriology (2008), 190 (10) pp 3588-3596. [PMID: 18326576]
- Yilmaz O, Yao L, Maeda K, et al. ATP scavenging by the intracellular pathogen Porphyromonas gingivalis inhibits P2X7-mediated host-cell apoptosis. Cell Microbiol (2008), 10 (4) pp 863-875. [PMID: 18005240]
- Zeqiraj E, Filippi BM, Goldie S, et al. ATP and MO25a Regulate the Conformational State of the STRADa Pseudokinase and Activation of the LKB1 Tumour Suppressor. PLoS Biol (2009), 7 (6). [PMID: 19513107]
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