Chromata ChIP Kit 25 Immunoprecipitations

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Chromatin Immunoprecipitation: Chromata ChIP ChIP Kit [NBP1-71709] - ChIP analysis of HeLa Nuclear Extracts. Image provided via product review by verified customer.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - ChIP analysis of HEK293 and SH-SY5Y cells. Image courtesy of an anonymous customer review.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - See notes for information on ChromataChIP Kit protocol information
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Ordering Information: NBP1-71709

  • Catalog Number
    NBP1-71709
  • Size(s)
    25 Immunoprecipitations
  • Availability
  • Price
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Chromata ChIP Kit Summary

Tested Applications ChIP
Specificity
This product contains all the necessary components to perform 25 Immunoprecipitation experiments including a positive control antibody (NB21-1023SS).
Kit Type
ChIP Kit
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.

Kit Components

Species Reactivity


Reviewed Species Read 2 rated 4.5
using NBP1-71709 in the following species:

Publications Read Publications using NBP1-71709 in the following species:


Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
Application Notes
This ChromataChIP kit is a useful tool for Chromatin Immunoprecipitation.
Reviewed Applications
Read 2 Review rated 4.5
using
NBP1-71709 in the following application:

Publications
Read Publications using
NBP1-71709 in the following applications:

Contact Information

Product PDFs

Reviews

Average Rating: 4.5
Recent Reviews
ChIP Tested In Human
Reviewed On: 05/07/2013
Rated: 4

Chromata NBP1-71709
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Publications

Chromata Kit (NBP1-71709) has been mentioned in at least 5 publications.
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Notes

Click here for detailed information on the ChromataChIP kit View the complete ChromatChIP Kit components and protocol here.

Packaging, Storage & Formulations

Storage
Storage is content dependent.
Buffer
Dependent on kit component.
Unit Size
25 Immunoprecipitations
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Background

This ChIP kit is a complete pack to help you perfom Chromatin Immunoprecipitation efficiently and accurately in your laboratory. We've taken all the guesswork out of ChIP by testing multiple component options to find the best possible component for you, the customer.

Reviews for Chromata Kit (NBP1-71709) (2) 4.52

Read what people are saying who have used Chromata Kit (NBP1-71709).

We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1: Chromatin Immunoprecipitation.
Average Rating: 4.5
(Based on 2 reviews)

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Images Ratings Applications Species Reviewed By Date Details
Chromatin Immunoprecipitation Chromata NBP1-71709
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Very Good
ChIP Human Corey. 05/07/2013
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedHeLa nuclear extract
Sample Pretreatment3,000,000 cells
SpeciesHuman
Lot031413
CommentsI generally had a good experience with the kit.

Primary Anitbody

Dilution Ratio5ug, overnight 4C

Details

Detection NotesqPCR
Chromatin Immunoprecipitation Chromata NBP1-71709
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Excellent
ChIP Human Jayashree Viswanathan 10/19/2012
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedHEK293 and SH-SY5Y cells
SpeciesHuman
CommentsThis kit is extremely easy to use and really WORKS! I have struggled with kits in the past but the beads in this kit are superior to some other vendors and the positive control antibody and primers are brilliant. Also I have tried various reverse-crosslinking parameters but incubation at 95C for 15 minutes as stated in the protocol works perfectly!

Primary Anitbody

Dilution RatioH3K4me3 antibody provided in the kit.

Details

Detection NotesRT-PCR using RPL30 positive control primers provided in the kit.

Publications for Chromata Kit (NBP1-71709) (5)

Read what people are writing who have used Chromata Kit (NBP1-71709).

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 applications: ChIP.

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Product General Protocols

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Video Protocols

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FAQs for Chromata (1)

  1. I was wondering if any of the Lightning-Link kit components contain phosphates, and whether you can tell me the concentration?
    • The Lightning-Link kits do contain phosphate. The final concentration will vary depending on the amount of antibody you add to the vial, but should always be less than 15mM.

Additional Products by Application

Additional Chromata ChIP Kit Products

Chromata NBP1-71709

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