Choline Kinase beta Antibody 0.05 mg

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Western Blot: CHKL Antibody [H00001120-B01P] - analysis of CHKB expression in transfected 293T cell line by CHKB MaxPab polyclonal antibody. Lane 1: CHKB transfected lysate (43.45 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: CHKL Antibody [H00001120-B01P] - Immunofluorescence of purified MaxPab antibody to CHKB on HeLa cell. [antibody concentration 10 ug/ml]
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Ordering Information: H00001120-B01P

  • Catalog Number
    H00001120-B01P
  • Displayed Format
    Unconjugated
  • Size(s)
    0.05 mg
  • Availability
  • Price
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Choline Kinase beta Antibody Summary

Species Human
Tested Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Mouse
Gene
CHKB
Purity
Protein A purified
Specificity
Reacts with choline kinase beta.
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Choline Kinase beta Antibody Details

Immunogen
CHKB (NP_005189.2, 1 a.a. ~ 395 a.a) full-length human protein.MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRDAERRAYQWCREYLGGAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYIPSRPLKTQELREPVLSAAIATKMAQFHGMEMPFTKEPHWLFGTMERYLKQIQDLPPTGLPEMNLLEMYSLKDEMGNLRKLLESTPSPVVFCHNDIQEGNILLLSEPE

Species Reactivity

Human

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
This antibody is reactive against transfected lysate in western blot, immunofluorescence, and as a detection antibody in ELISA.

Contact Information

Product PDFs

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Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
1x PBS, pH 7.4
Unit Size
0.05 mg
Concentration
mg/ml
Preservative
No Preservative
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol CHKB
Entrez
Uniprot

Alternate Names for Choline Kinase beta Antibody

  • CHETKcholine/ethanolamine kinase beta
  • CHKLcholine kinase-like
  • choline kinase betaEK
  • Choline kinase-like protein
  • CK
  • CKB
  • CKEKBEKBcholine/ethanolamine kinase
  • EC 2.7.1.32
  • EC 2.7.1.82
  • Ethanolamine kinase
  • Ethanolamine kinase beta
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Related Products by Gene

Background

Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for Choline Kinase beta (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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