Cdk2 Antibody (H00001017-M02)

Cdk2 Antibody (2E8)

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Cdk2 Antibody (2E8) Summary:
Species:Hu
Applications:ELISA, RNAi, WB
Clonality:Monoclonal
Gene:CDK2
Purity:IgG purified
Host:Mouse
Specificity:CDK2 - cyclin-dependent kinase 2
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Cdk2 Antibody (2E8) Details:
Clone:2E8
Isotype:IgG1 Kappa
Immunogen:CDK2 (AAH03065, 211 a.a. ~ 298 a.a) partial recombinant protein with GST tag.
Localization:Cytoplasmic and Nuclear
Epitope:QLFRIFRTLGTPDEVVWPGVTSMPDYKPSF PKWARQDFSKVV PPLDEDGRSLLSQMLH YDPNKRISAKAALAHPFFQDVTKPVP HL RL*
Species Reactivity:

Human. Other species not tested.

Applications:
Uses:Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Dilutions:ELISA, Western Blot 1:500, RNAi Validation
Unit Size:0.1 mg
Concentration:Please see the vial label for concentration.
Notes:
This product is produced by and distributed for Abnova, a company based in Taiwan.
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:Phosphate buffered saline, pH 7.2
Preservative:No Preservative
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: CDK2
Entrez1017 (Human)
OMIM116953 (Human)
Swiss ProtAAH03065 (Human)
 
Background:

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

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Working with Cdk2

Cdk2 Antibody (2E8) Images (5)
Western blot analysis of CDK2 over-expressed 293 cell line. Lane 2 cotransfected with CDK2 Validated Chimera RNAi or non-transfected control. Blot probed with H00001017-M02. GAPDH (36.1 kDa) used as loading control.Western Blot analysis of CDK2 expression in transfected 293T cell line by CDK2 monoclonal antibody (M02), clone 2E8.

Lane 1: CDK2 transfected lysate(33.9 KDa).
Lane 2: Non-transfected lysate.
CDK2 monoclonal antibody (M02), clone 2E8. Western Blot analysis of CDK2 expression in Jurkat ( Cat # L017V1 ).CDK2 monoclonal antibody (M02), clone 2E8 Western Blot analysis of CDK2 expression in HL-60 ( Cat # L014V1 ).
Detection limit for recombinant GST tagged CDK2 is approximately 0.03ng/ml as a capture antibody.
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Jump to: Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Cdk2 in Context


Research Areas related to Cdk2 (4):
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