This Carbonic Anhydrase IX antibody is useful for Western blot, Immunofluorescence/Immunocytochemistry, and Immunohistochemistry. Gel Super Shift Assays was reported in scientific literature. In Western blot a band is observed ~53 kDa.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Carbonic anhydrase IX (CAIX) is a member of the Carbonic anhydrase family which are enzymes that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. They are abundant in all mammalian tissues and because of their functionality, they have become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). Carbonic Anhydrase IX (CA IX) is one of the most hypoxically inducible genes because of its stability and the location of the expressed protein within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX (CA9) results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.
Reviews for Carbonic Anhydrase IX/CA9 Antibody (NB100-417) (3)
Read what people are saying who have used Carbonic Anhydrase IX/CA9 Antibody (NB100-417).
We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2: Western Blot, Immunocytochemistry/Immunofluorescence.
Average Rating: 4.7 (Based on 3 reviews)
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FAQs for Carbonic Anhydrase IX/CA9 (9)
How many units do we need to purchase in order to stain 150 formalin fixed, paraffin embedded kidney biopsies? It depends on the amount used per slide, so could you please confirm how much antibody do you use per slide in IHC-P? Also, is the ab compatible with ENVISION FLEX+, HIGH pH, FOR AS?
Unfortunately there is no number that I can provide to you as it is impossible to know for sure how many slides you will be able to stain with a vial. This will depend on your experimental conditions, your samples and how highly expressed the protein is, and what dilution you end up choosing. This antibody is recommended to be used at a 1:1000 dilution for IHC staining as this has worked well for our lab. You should order a single vial and test to determine what amount will be best for your assay rather than order too much or too little without first working with the antibody. As for your second question, that is not something that our lab has tested with so I am unsure if it is compatible or not. It has a fairly normal range pH in the buffer (7-8) so most likely the functions best around that range and not at a high pH.
Could you please let me know the immunogen peptide location for this Ab?
The immunogen used to create this antibody was made to a cytoplasmic area of the protein.
I'm going to use your CA-9 Antibody for paraffin-Immunohistochemistry. Do I have to do any special pretreatments?
Here is our protocol specific for this product. However, we recommend antigen retrieval to ensure the epitope is not masked, and we also recommend that you add a permeablizing agent to access the epitope in the nucleus.
We dilute our antibodies in 5% milk TBS-Tween and usually freeze-thaw them multiple times. Is this possible with this antibody when diluted?
Freezing is not recommended for NB100-417 under any circumstances. Unfortunately, the antibody would no longer be covered by our guarantee if it was stored at a temperature that did not match our storage instructions. Of course, you are free to test your proposed conditions if you would like, but we would not be able to accept responsibility if the antibody was damaged by this.
Is this antibody is carrier-free? If not, do you have a carrier-free version?
NB100-417 is carrier protein free, but does contain 0.1% sodium azide. The buffer formulation is: Tris-citrate/phosphate, pH 7-8 with 0.1% sodium azide. The following products are carrier protein and azide free (catalog numbers 23300002, H00000768-B01P and H00000768-D01P). If you would like to use NB100-417, we do offer antibody purification kits to remove unwanted buffer ingredients. See this link here for those products.
I have a couple of questions regarding the Carbonic Anhydrase IX Antibody (NB100-417) listed on your website. We are wondering if this antibody is carrier-free, and if not, whether you have a carrier-free version?
Our NB100-417 is carrier protein free but does contain 0.1% sodium azide. The buffer formulation is: Tris-citrate/phosphate, pH 7-8 with 0.1% sodium azide. The following products are carrier protein and azide free (catalog numbers 23300002, H00000768-B01P and H00000768-D01P). If you would like to use NB100-417, we do offer antibody purification kits to remove unwanted buffer ingredients. See the link here for those products: AbSelect.
I am interested in using the CA9 antibody (NB100-417) that you supply. We dilute our antibodies in 5% milk TBS-Tween and usually freeze-thaw them multiple times. Is this possible with this antibody when diluted?
I have checked with the lab and they have informed me that freezing is not recommended for NB100-417 under any circumstances. Unfortunately, the antibody would no longer be covered by our guarantee if it was stored at a temperature that did not match our storage instructions. Of course, you are free to test your proposed conditions if you would like, but we would not be able to accept responsibility if the antibody was damaged by this.
In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
I was wondering what does Format 7C mean as far as anitbodies go?
7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)