Carbonic Anhydrase IX/CA9 Antibody 0.1 ml Hypoxia Marker

Click image to view larger
Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis using the HRP conjugate of NB100-417. Detection of carbonic anhydrase IX in transfected HEK cell lysate using NB 100-417. Rabbit IgG was used as a negative control.
Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis using the HRP conjugate of NB100-417. Staining of HEK 293 cells using NB100-417. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock transfected cells.
Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis using the Biotin conjugate of NB100-417. Staining of Renal carcinoma tissue with polyclonal carbonic anhydrase IX, NB 100-417.
Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis using the DyLight 488 conjugate of NB100-417. Staining of Carbonic Anhydrase IX (red) in human glioma U87 cells. DAPI counterstains nuclei (blue). Image provided via product review by verified customer.
Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis of Carbonic Anhydrase IX expression in 1) HeLa, 2) MDA-MB-231 and 3) A549 whole cell lysates.
Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Detection of carbonic anhydrase IX in transfected HEK cell lysate using NB 100-417. Rabbit IgG was used as a negative control.
Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis of CAIX on rat renal cortex using NB100-417.
Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Carbonic Anhydrase IX on various human cell lysates. Image from verified customer review.
Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Detection of  HRP conjugated carbonic anhydrase IX in transfected HEK cell lysate. Rabbit IgG was used as a negative control.
Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis of Biotin conjugated antibody on HEK 293 cells. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock transfected cells.
Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Immunofluorescence of human RCC tumor cryosections using NB100-417 (Panel A). Panel B shows staining with normal rabbit serum.
Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis using the Biotin conjugate of NB100-417. Staining of HEK 293 cells using NB100-417. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock transfected cells.
Immunohistochemistry-Frozen: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis of human colon carcinoma, xenografted in mice. Image courtesy of an anonymous product review.
Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis using the Biotin conjugate of NB100-417. Staining of human RCC tumor cryosections using NB100-417 (Panel A). Panel B shows staining with normal rabbit serum.
Simple Western: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Simple Western lane view shows a specific band for CAIX in 0.1 mg/ml of HeLa lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Download Datasheet
See All Related

Ordering Information: NB100-417

  • Catalog Number
    NB100-417
  • Displayed Format
    Unconjugated
  • Other Formats
  • Size(s)
  • Availability
  • Price
    Shipping
  

Carbonic Anhydrase IX/CA9 Antibody Summary

Species Human, Mouse, Rat, Canine, Plant
Tested Applications WB, Simple Western, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, ChIP
Clonality
Polyclonal
Host
Rabbit
Gene
CA9
Purity
Immunogen affinity purified
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Carbonic Anhydrase IX/CA9 Antibody Details

Marker
Hypoxia Marker
Immunogen
A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-500) [UniProt Q16790]
Localization
Membrane.

Species Reactivity

Human, dog, rat and mouse (PMID 22842475). Plant reactivity reported in scientific literature (PMID: 19278636)
Reviewed Species Read 3 rated 4.7
using NB100-417 in the following species:

Publications Read Publications using NB100-417 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Simple Western 1:50
  • ELISA
  • Flow Cytometry
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
This Carbonic Anhydrase IX antibody is useful for Western blot, Immunofluorescence/Immunocytochemistry, and Immunohistochemistry. Gel Super Shift Assays was reported in scientific literature. In Western blot a band is observed ~53 kDa.In Simple Western only 10-15 uL of the recommended dilution is used per data point. Use in ELISA reported in scientific literature (PMID 19963243). Use in chromatin immunoprecipitation reported in multiple pieces of scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Molecular Weight
55 kDa
Positive Controls
Other Available
Formats
Alexa Fluor (R) 405 Labeled NB100-417AF405
Alexa Fluor (R) 488 Labeled NB100-417AF488
Alexa Fluor (R) 647 Labeled NB100-417AF647
Alexa Fluor (R) 700 Labeled NB100-417AF700
Biotin Labeled NB100-417B
DyLight 405 Labeled NB100-417V
DyLight 488 Labeled NB100-417G
DyLight 550 Labeled NB100-417R
DyLight 650 Labeled NB100-417C
FITC Labeled NB100-417F
HRP Labeled NB100-417H
Control Peptide
Carbonic Anhydrase IX/CA9 Peptide (NB100-417PEP)
Reviewed Applications
Read 3 Review rated 4.7
using
NB100-417 in the following applications:

Publications
Read Publications using
NB100-417 in the following applications:

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Average Rating: 4.7
Recent Reviews
WB Tested In Human
Reviewed On: 07/21/2014
Rated: 5

Carbonic Anhydrase IX/CA9 NB100-417
Image 1 of 2
Earn rewards points by submitting your review for Carbonic Anhydrase IX/CA9 Antibody (NB100-417)

Publications

Carbonic Anhydrase IX/CA9 Antibody (NB100-417) has been mentioned in at least 108 publications.
Earn rewards if you have published using Carbonic Anhydrase IX/CA9 Antibody (NB100-417).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Unit Size
0.1 ml
Concentration
1.0 mg/ml
Preservative
0.025% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol CA9
Entrez
Uniprot

Alternate Names for Carbonic Anhydrase IX/CA9 Antibody

  • CA9
  • CA-IX
  • CAIXcarbonic anhydrase 9
  • Carbonate dehydratase IX
  • carbonic anhydrase IXpMW1
  • carbonic dehydratase
  • EC 4.2.1.1
  • G250
  • Membrane antigen MN
  • MN
  • MNRCC-associated antigen G250
  • P54/58N
  • RCC
  • RCC-associated protein G250
  • Renal cell carcinoma-associated antigen G250
Show More

Related Products by Gene

Background

Carbonic anhydrase IX (CAIX) is a member of the Carbonic anhydrase family which are enzymes that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. They are abundant in all mammalian tissues and because of their functionality, they have become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). Carbonic Anhydrase IX (CA IX) is one of the most hypoxically inducible genes because of its stability and the location of the expressed protein within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX (CA9) results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

Reviews for Carbonic Anhydrase IX/CA9 Antibody (NB100-417) (3) 4.73

Read what people are saying who have used Carbonic Anhydrase IX/CA9 Antibody (NB100-417).

We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2: Western Blot, Immunocytochemistry.
Average Rating: 4.7
(Based on 3 reviews)

Have you used Carbonic Anhydrase IX/CA9 Antibody (NB100-417)? Submit your review and earn rewards points which can be used for merchandise & discounts.

Sort 3 reviews for Carbonic Anhydrase IX/CA9 Antibody (NB100-417) by:
Filter by Ratings
Excellent
(2)
Very Good
(1)
All Ratings
Filter by Applications
WB
(2)
ICC
(1)
All Applications
Filter by Species
Human
(3)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
WB Human rajesha rupaimoole 12/10/2014
Show

Summary

ApplicationWestern Blot
Sample TestedA2780 ovarian cancer cells
SpeciesHuman

Blocking

Blocking Details5% BSA

Details

Detection NotesECL
Western Blot Carbonic Anhydrase IX/CA9 NB100-417
Click to Enlarge
Excellent
WB Human Xiuquan Luo 07/21/2014
Show

Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate
Sample PretreatmentNo treatment
SpeciesHuman

Blocking

Blocking Details1xPBST with 5% non-fat milk, RT 1 hour

Primary Anitbody

Dilution Ratio1xpBST with 5% non-fat milk, overnight 4C, 1:1000 dilution

Secondary Antibody

Secondary Manufacturer Cat#sc-2004
Secondary Concentration1:2000 dilution

Details

Detection NotesECL, exposured for 3 min
Immunocytochemistry Carbonic Anhydrase IX/CA9 NB100-417
Click to Enlarge
Very Good
ICC Human Sonia Rocha 12/21/2011
Show

Summary

ApplicationImmunocytochemistry
Sample Testedhuman cells
Sample Pretreatmentuntreated
SpeciesHuman
CommentsReally nice results using microscopy.

Blocking

Blocking DetailsBlocking Buffer: 1% Donkey serum PBS, Blocking Time: 30 minutes, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: PBS/1% Donkey serum, Dilution Ratio: 1:250, Incubation Time: 1 hour, Incubation Temp: 20 C

Details

Detection NotesDetection Visualization Method: fluorescence at 488, Negative Control: secondary and primary only
Fixation DetailsSample Preparation: PFA fixed

Publications for Carbonic Anhydrase IX/CA9 Antibody (NB100-417) (108)

Read what people are writing who have used Carbonic Anhydrase IX/CA9 Antibody (NB100-417).

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Plant.

We have publications tested in 7 applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P.

Have you published using Carbonic Anhydrase IX/CA9 Antibody (NB100-417)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
WB
(14)
ChIP
(2)
ELISA
(1)
ICC/IF
(8)
IHC
(35)
IHC-Fr
(4)
IHC-P
(33)
All Applications
Filter By Species
Human
(73)
Mouse
(11)
Rat
(4)
Plant
(1)
All Species
Publication Applications Species
Sathornsumetee S, Cao Y, Marcello JE et al. Tumor angiogenic and hypoxic profiles predict radiographic response and survival in malignant astrocytoma patients treated with bevacizumab and irinotecan. J Clin Oncol;26(2):271-8. 2008 Jan 10. [PMID: 1818266] (IHC-P, Human) IHC-P Human
Atkins M, Regan M, McDermott D et al. Carbonic anhydrase IX expression predicts outcome of interleukin 2 therapy for renal cancer. Clin Cancer Res;11(10):3714-21. 2005 May 15. [PMID: 15897568] (IHC, Human) IHC Human
Van den Eynden GG, Van der Auwera I, Van Laere SJ et al. Angiogenesis and hypoxia in lymph node metastases is predicted by the angiogenesis and hypoxia in the primary tumour in patients with breast cancer. Br J Cancer;93(10):1128-36. 2005 Nov 14. [PMID: 16251878] (IHC-P, Human) IHC-P Human
Chamboredon S, Ciais D, Desroches-Castan A et al. Hypoxia-inducible Factor-1{alpha} mRNA: a new target for destabilization by tristetraprolin in endothelial cells. Mol Biol Cell. 2011 Jul 20. [PMID: 21775632] (WB, Human) WB Human
Garcia-Parra R, Wood D, Shah RB et al. Investigation on tumor hypoxia in resectable primary prostate cancer as demonstrated by (18)F-FAZA PET/CT utilizing multimodality fusion techniques. Eur J Nucl Med Mol Imaging. 2011 Jul 16. [PMID: 21833840] (IHC-P, Human) IHC-P Human
Ogino T, Onishi H, Suzuki H et al. Inclusive estimation of complex antigen presentation functions of monocyte-derived dendritic cells differentiated under normoxia and hypoxia conditions. Cancer Immunology, Immunotherapy: CII. 2011 Sep 20. [PMID: 21932134]
Deschamps L, Bacha D, Rebours V, Mebarki M, Bretagnol F, Panis Y, Bedossa P, Ruszniewski P, Couvelard A. The Expression of the Hypoxia Markers CA9 and CXCR4 Is Correlated with Survival in Patients with Neuroendocrine Tumours of the Ileum. Neuroendocrinology. 2011 Nov 29. [PMID: 22133596] (IHC, Human) IHC Human
Matsumura Y, Ishii G, Aokage K, Kuwata T, Hishida T, Yoshida J, Nishimura M, Nagai K, Ochiai A. Morphophenotypic characteristics of intralymphatic cancer and stromal cells susceptible to lymphogenic metastasis. Cancer Sci. 2012 Mar 16. [PMID: 22429811] (IHC, Human) IHC Human
Speisky D, Duces A, Bieche I et al. Molecular Profiling of Pancreatic Neuroendocrine Tumors in Sporadic and Von Hippel-Lindau Patients. Clin Cancer Res;18(10):2838-2849. 2012 May 15. [PMID: 22461457] (IHC, Human) IHC Human
Pellegrini L, Pucci B, Villanova L, Marino ML, Marfe G, Sansone L, Vernucci E, Bellizzi D, Reali V, Fini M, Russo MA, Tafani M. SIRT3 protects from hypoxia and staurosporine-mediated cell death by maintaining mitochondrial membrane potential and intracellular pH. Cell Death Differ. 2012 May 18. [PMID: 22595756] (WB, Human) WB Human
Muriel Lopez C, Esteban E, Astudillo A, Pardo P, Berros JP, Izquierdo M, Crespo G, Fonseca PJ, Sanmamed M, Martinez-Camblor P. Predictive factors for response to treatment in patients with advanced renal cell carcinoma. Invest New Drugs. 2012 May 27. [PMID: 22644070] (IHC, Human) IHC Human
Melvin A, Mudie S, Rocha S. The chromatin remodeler ISWI regulates the cellular response to hypoxia, role of FIH. Mol Biol Cell. 2011 Sep 7. [PMID: 21900490] (Human) Human
Yoo H, Baia GS, Smith JS et al. Expression of the hypoxia marker carbonic anhydrase 9 is associated with anaplastic phenotypes in meningiomas. Clin Cancer Res;13(1):68-75. 2007 Jan 1. [PMID: 17200340] (IHC-P, Human) IHC-P Human
Li Y, Wang H, Tu C et al. Role of hypoxia and EGF on expression, activity, localization and phosphorylation of carbonic anhydrase IX in MDA-MB-231 breast cancer cells. Biochim Biophys Acta. 2010 Oct 1. [PMID: 20920536] (WB, Human) WB Human
Guise CP, Abbattista MR, Tipparaju SR et al. Diflavin Oxidoreductases Activate the Bioreductive Prodrug PR104A Under Hypoxia. Molecular Pharmacology. 2011 Oct 7. [PMID: 21984255]
Onishi H, Morifuji Y, Kai M, Suyama K, Iwasaki H, Katano M. Hedgehog inhibitor decreases chemosensitivity to 5-fluorouracil and gemcitabine under hypoxic conditions in pancreatic cancer. Cancer Sci. 2012 Apr 6. [PMID: 22486854] (IHC, ICC/IF, Mouse) ICC/IF, IHC Mouse
Al-Ahmadie HA, Alden D, Fine SW et al. Role of immunohistochemistry in the evaluation of needle core biopsies in adult renal cortical tumors: an ex vivo study. Am. J. Surg. Pathol. 2011 Jul. [PMID: 21677535] (IHC, Human) IHC Human
Westbury CB, Pearson A, Nerurkar A et al. Hypoxia can be detected in irradiated normal human tissue: a study using the hypoxic marker pimonidazole hydrochloride. Br J Radiol;80(959):934-8. 2007 Nov. [PMID: 17908818] (IHC-P, Human) IHC-P Human
Kaidi A, Moorghen M, Williams AC et al. Is the downregulation of EphB2 receptor expression during colorectal tumorigenesis due to hypoxia?. Gut 56(11):1637-8. 2007 Nov. [PMID: 17938437] (IHC-P, Human) IHC-P Human
Sakata K, Someya M, Nagakura H et al. Brachytherapy for oral tongue cancer: an analysis of treatment results with various biological markers. Jpn J Clin Oncol;38(6):402-7. 2008 Jun. [PMID: 18573850] (IHC-P, Human) IHC-P Human
Lapinski JE, Chen L, Zhou M. Distinguishing clear cell renal cell carcinoma, retroperitoneal paraganglioma, and adrenal cortical lesions on limited biopsy material: utility of immunohistochemical markers. Appl Immunohistochem Mol Morphol;18(5):414-21. 2010 Oct. [PMID: 20861762] (IHC, Human) IHC Human
Mackenzie KA, Miller AP, Hock BD, Gardner J, Simcock JW, Roake JA, Dachs GU, Robinson BA, Currie MJ. Angiogenesis and host immune response contribute to the aggressive character of non-melanoma skin cancers in renal transplant recipients. Histopathology;58(6):875-85. 2011 May. [PMID: 21585427] (IHC, Human) IHC Human
Jung JH, Kim HJ, Yeom J, Yoo C, Shin J, Yoo J, Kang CS, Lee C. Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer. J Gastroenterol;47(1):37-48. 2012 Jan. [PMID: 22015694] (WB, Human) WB Human
Lim CS, Kiriakidis S, Paleolog EM, Davies AH. Increased activation of the hypoxia-inducible factor pathway in varicose veins. J Vasc Surg;55(5):1427-1439e1. 2012 May. [PMID: 22277691] (WB, Human) WB Human
Banuelos CA, Banath JP, Kim J-Y et al. {gamma}H2AX Expression in Tumors Exposed to Cisplatin Fractionated Irradiation. Clin Cancer Res;15(10):3344-3353. 2009 [PMID: 19401347] (IHC-P, Human) IHC-P Human
Chen J, Kobayashi M, Darmanin S et al. Hypoxia-Mediated Up-Regulation of Pim-1 Contributes to Solid Tumor Formation. Am J Pathol;175(1):400-411. 2009 [PMID: 19528349] (IHC-P, Mouse) IHC-P Mouse
Loinard C, Ginouves A, Vilar J et al. Inhibition of Prolyl Hydroxylase Domain Proteins Promotes Therapeutic Revascularization. Circulation;120(1):50-59. 2009 [PMID: 19546390] (WB, Human, Mouse) WB Human, Mouse
Lucio-Eterovic AK, Piao Y, de Groot JF. Mediators of Glioblastoma Resistance Invasion during Antivascular Endothelial Growth Factor Therapy. Clin Cancer Res:1078-0432.CCR-1009-0575. 2009 [PMID: 19567589] (IHC-P, Human) IHC-P Human
Benito J, Shi Y, Szymanska B et al. Pronounced Hypoxia in Models of Murine and Human Leukemia: High Efficacy of Hypoxia-Activated Prodrug PR-104. PLoS ONE. 2011 [PMID: 21853076]
Narita T, Yin S, Gelin CF et al. Identification of a Novel Small Molecule HIF-1{alpha} Translation Inhibitor. Clin Cancer Res 9;15(19):6128-6136. 200 [PMID: 19789328] (WB, Human) WB Human
Chaumeil MM, Ozawa T, Park I et al. Hyperpolarized 13C MR spectroscopic imaging can be used to monitor Everolimus treatment in vivo in an orthotopic rodent model of glioblastoma. NeuroImage. [PMID: 21807103] (IHC-P, Rat) IHC-P Rat
Wang Y, Roche O, Xu C et al. Hypoxia promotes ligand-independent EGF receptor signaling via hypoxia-inducible factor-mediated upregulation of caveolin-1 Proc Natl Acad Sci U S A 2012 Mar 12 [PMID: 22411794] (EMSA, WB, Human) WB Human
Eguchi D, Ikenaga N, Ohuchida K et al. Hypoxia enhances the interaction between pancreatic stellate cells and cancer cells via increased secretion of connective tissue growth factor J Surg Res 2012 Jul 6 [PMID: 22795353] (IHC, Human) IHC Human
Fromy B, Lingueglia E, Sigaudo-Roussel D et al. Asic3 is a neuronal mechanosensor for pressure-induced vasodilation that protects against pressure ulcers Nat Med 2012 Jul 29 [PMID: 22842475] (IHC, Mouse) IHC Mouse
Engler JR, Robinson AE, Smirnov I et al. Increased microglia/macrophage gene expression in a subset of adult and pediatric astrocytomas PLoS One 2012 [PMID: 22937035] (IHC, Human) IHC Human
Weber DC, Tille JC, Combescure C et al. The prognostic value of expression of HIF1alpha, EGFR and VEGF-A, in localized prostate cancer for intermediate- and high-risk patients treated with radiation therapy with or without androgen deprivation therapy Radiat Oncol 2012 Apr 30 [PMID: 22546016] (IHC, Human) IHC Human
Brouillet S, Murthi P, Hoffmann P et al. EG-VEGF controls placental growth and survival in normal and pathological pregnancies: case of fetal growth restriction (FGR) Cell Mol Life Sci 2012 Sep 2 [PMID: 22941044] (IHC, Human) IHC Human
Muriel Lopez C, Esteban E, Berros JP et al. Prognostic Factors in Patients With Advanced Renal Cell Carcinoma Clin Genitourin Cancer 2012 Sep 6 [PMID: 22959659] (IHC, Human) IHC Human
Piao Y, Liang J, Holmes L et al. Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype Neuro Oncol 2012 Sep 10 [PMID: 22965162] (IHC, Human, Mouse) IHC Human, Mouse
Currie MJ, Beardsley BE, Harris GC et al. Immunohistochemical analysis of cancer stem cell markers in invasive breast carcinoma and associated ductal carcinoma in situ: relationships with markers of tumor hypoxia and microvascularity Hum Pathol 2012 Oct 1 [PMID: 23036368] (IHC, Human) IHC Human
Proescholdt MA, Merrill MJ, Stoerr EM et al. Function of carbonic anhydrase IX in glioblastoma multiforme Neuro Oncol 2012 Nov [PMID: 23074198] (IHC, WB, Human) IHC, WB Human
Kato Y, Yashiro M, Noda S et al. Expression of a hypoxia-associated protein, carbonic anhydrase-9, correlates with malignant phenotypes of gastric carcinoma. Digestion 2010 [PMID: 20588040] (IHC, Human) IHC Human
Arvidsson Y, Bergstrom A, Arvidsson L et al. Hypoxia stimulates CXCR4 signalling in ileal carcinoids. Endocr Relat Cancer 2010 Jun 1 [PMID: 20071457] (IHC, Human) IHC Human
Pinato DJ, Ramachandran R, Toussi ST et al. Immunohistochemical markers of the hypoxic response can identify malignancy in phaeochromocytomas and paragangliomas and optimize the detection of tumours with VHL germline mutations Br J Cancer 2012 Dec 20 [PMID: 23257898] (IHC, Human) IHC Human
Delhaas T, Van der Meer SF, Schaart G et al. Steep Increase in Myonuclear Domain Size During Infancy Anat Rec (Hoboken) 2012 Dec 4 [PMID: 23213045] (IHC, ICC/IF, Human) ICC/IF, IHC Human
Yin S, Kaluz S, Devi NS et al. Arylsulfonamide KCN1 Inhibits In Vivo Glioma Growth and Interferes with HIF Signaling by Disrupting HIF-1alpha Interaction with Cofactors p300/CBP Clin Cancer Res 2012 Dec 15 [PMID: 22923450] (WB, Human) WB Human
Zhang Q, Bai X, Chen W et al. Wnt/beta-catenin signaling enhances hypoxia-induced epithelial-mesenchymal transition in hepatocellular carcinoma via crosstalk with hif-1alpha signaling. Carcinogenesis 2013 Jan 27 [PMID: 23358852] (WB, Human) WB Human
Aarntzen EH, Srinivas M, Bonetto F et al. Targeting of 111In-Labeled Dendritic Cell Human Vaccines Improved by Reducing Number of Cells. Clin Cancer Res 2013 Mar 15 [PMID: 23382117] (IHC, Human) IHC Human
Cho SY, Lee HJ, Lee HJ et al. Activation of AMP-Activated Protein Kinase alpha and Extracelluar Signal-Regulated Kinase Mediates CB-PIC-Induced Apoptosis in Hypoxic SW620 Colorectal Cancer Cells Evid Based Complement Alternat Med 2013 [PMID: 23589723] (IHC-P, Human) IHC-P Human
Li Y, Wang H, Oosterwijk E et al. Antibody-specific detection of CAIX in breast and prostate cancers. Biochem Biophys Res Commun 2009 Aug 28 [PMID: 19538935] (IHC-P, Human) IHC-P Human
Carbonell WS, Delay M, Jahangiri A et al. beta1 Integrin Targeting Potentiates Antiangiogenic Therapy and Inhibits the Growth of Bevacizumab-Resistant Glioblastoma. Cancer Res 2013 May 15 [PMID: 23644530] (IHC-P, ICC/IF, Human) ICC/IF, IHC-P Human
Baia GS, Dinca EB, Ozawa T et al. An orthotopic skull base model of malignant meningioma. Brain Pathol 2008 Apr [PMID: 18093250] (IHC-P, Mouse) IHC-P Mouse
Gauter-Fleckenstein B, Fleckenstein K, Owzar K et al. Comparison of two Mn porphyrin-based mimics of superoxide dismutase in pulmonary radioprotection. Free Radic Biol Med 2008 Mar 15 [PMID: 18082148] (IHC-P, Rat) IHC-P Rat
Bussolati B, Lauritano C, Moggio A et al. Renal CD133+/CD73+ Progenitors Produce Erythropoietin under Hypoxia and Prolyl Hydroxylase Inhibition. J Am Soc Nephrol 2013 May 9 [PMID: 23661806]
Campanile C, Arlt MJ, Kramer SD et al. Characterization of Different Osteosarcoma Phenotypes by PET Imaging in Preclinical Animal Models. J Nucl Med 2013 Jun 25 [PMID: 23801674]
Kumar S, Mokhtari RB, Oliveira ID et al. Tumor Dynamics in Response to Antiangiogenic Therapy with Oral Metronomic Topotecan and Pazopanib in Neuroblastoma Xenografts. Transl Oncol 2013 Aug 1 [PMID: 23908692] (IHC, Human) IHC Human
Pistollato F, Abbadi S, Rampazzo E et al. Intratumoral hypoxic gradient drives stem cells distribution and MGMT expression in glioblastoma. Stem Cells 2010 May [PMID: 20309962] (IHC-P, Human) IHC-P Human
Pistollato F, Rampazzo E, Persano L et al. Interaction of hypoxia-inducible factor-1a and Notch signaling regulates medulloblastoma precursor proliferation and fate. Stem Cells 2010 Nov [PMID: 20827750] (IHC-P, Human) IHC-P Human
Dubois L, Peeters SG, van Kuijk SJ et al. Targeting carbonic anhydrase IX by nitroimidazole based sulfamides enhances the therapeutic effect of tumor irradiation: A new concept of dual targeting drugs. Radiother Oncol 2013 Jul 9 [PMID: 23849171] (IHC-Fr, Mouse) IHC-Fr Mouse
Park I, Hu S, Bok R et al. Evaluation of heterogeneous metabolic profile in an orthotopic Human glioblastoma xenograft model using compressed sensing hyperpolarized 3D 13C magnetic resonance spectroscopic imaging. Magn Reson Med 2013 Jul [PMID: 22851374] (IHC-P, Rat) IHC-P Rat
Albert S, Hourseau M, Halimi C et al. Prognostic value of the chemokine receptor CXCR4 and epithelial-to-mesenchymal transition in patients with squamous cell carcinoma of the mobile tongue. Oral Oncol 2012 Dec [PMID: 22776129]
Cioffi S, Martucciello S, Fulcoli FG et al. Tbx1 regulates brain vascularization. Hum Mol Genet. 2013 Sep 7 [PMID: 23945394] (IHC-Fr, ICC/IF, Mouse) ICC/IF, IHC-Fr Mouse
Fahling M, Mathia S, Paliege A et al. Tubular von Hippel-Lindau Knockout Protects against Rhabdomyolysis-Induced AKI. J Am Soc Nephrol. 2013 Aug 22 [PMID: 23970125]
Zhang M, Boughton P, Rose B et al. The Use of Porous Scaffold as a Tumor Model. Int J Biomater. 2013 [PMID: 24101930] (IHC, Human) IHC Human
Wen YA, Stevens PD, Gasser ML et al. Downregulation of PHLPP Expression Contributes to Hypoxia-Induced Resistance to Chemotherapy in Colon Cancer Cells. Mol Cell Biol. 2013 Nov [PMID: 24061475] (WB, Human) WB Human
El-Heliebi A, Kroneis T, Zohrer E et al. Are morphological criteria sufficient for the identification of circulating tumor cells in renal cancer?. J Transl Med. 2013 Sep 17 [PMID: 24044779] (ICC/IF, Human) ICC/IF Human
Pinato DJ, Tan TM, Toussi ST et al. An expression signature of the angiogenic response in gastrointestinal neuroendocrine tumours: correlation with tumour phenotype and survival outcomes. Br J Cancer. 2013 Nov 14 [PMID: 24231952] (IHC-P, Human) IHC-P Human
Chen X, Iliopoulos D, Zhang Q et al. XBP1 promotes triple-negative breast cancer by controlling the HIF1a pathway. Nature 4/3/2014 [PMID: 24670641] (IHC-P, Human) IHC-P Human
Kwon OJ, Park JJ, Ko GH et al. HIF-1a and CA-IX as predictors of locoregional control for determining the optimal treatment modality for early-stage laryngeal carcinoma. Head Neck 2/12/2014 [PMID: 24677746] (IHC-P, Human) IHC-P Human
Sadlecki P, Bodnar M, Grabiec M. The Role of Hypoxia-Inducible Factor-1a, Glucose Transporter-1,(GLUT-1) and Carbon Anhydrase IX in Endometrial Cancer Patients. BioMed Research International 3/12/2014 [PMID: 24745019] (IHC-P, Human) IHC-P Human
Motzer RJ, Hutson TE, Hudes GR et al. Investigation of novel circulating proteins, germ line single-nucleotide polymorphisms, and molecular tumor markers as potential efficacy biomarkers of first-line sunitinib therapy for advanced renal cell carcinoma. Cancer Chemother. Pharmacol. 2014 Aug 07 [PMID: 25100134] (IHC-P, Human)

Details:
Rabbit polyclonal Carbonic Anhydrase IX/CA-IX antibody used for IHC on formalin-fixed paraffin-embedded (FFPE) blocks or unstained slides from tumor specimens.
IHC-P Human
Chen LQ, Howison CM, Spier C et al. Assessment of carbonic anhydrase IX expression and extracellular pH in B-cell lymphoma cell line models. Leuk. Lymphoma. 2014 Aug 17 [PMID: 25130478]
Hwa JS, Kwon OJ, Park JJ et al. The prognostic value of immunohistochemical markers for oral tongue squamous cell carcinoma. Eur Arch Otorhinolaryngol. 2014 Aug 29 [PMID: 25169079]
Santos Cd, Tijeras-Raballand A, Serova M et al. Effects of preset sequential administrations of sunitinib and everolimus on tumour differentiation in Caki-1 renal cell carcinoma. Br. J. Cancer. 2014 Nov 25 [PMID: 25422908] (IHC-P, Human) IHC-P Human
Lin XY, Zhang H, Tang N et al. Expression and diagnostic implications of carbonic anhydrase IX in several tumours with predominantly clear cell morphology. Histopathology. 2014 Nov 27 [PMID: 25431204] (IHC-P, Human)

Details:
Carbonic Anhydrase IX/CA9 antibody used for IHC-P on sections of clear cell renal cell carcinoma (ccRCC; primary and metastatic) and several other type of tumors with predominantly clear cell morphology - tissues previously fixed in 10% neutral buffered formalin and embedded in paraffin, 4um sections, routine IHC-P processing with streptavidin-peroxidase detection system. ccRCC, and breast / pulmonary/hepatic clear cell carcinoma, urothelial carcinoma with clear cell change, clear cell meningioma/ependymoma, haemangioblastoma, and clear cell hidradenoma showed expression of CAIX. See full text for staining images and the expression levels.
IHC-P Human
Gellert LL, Mehra R, Chen YB et al. The diagnostic accuracy of percutaneous renal needle core biopsy and its potential impact on the clinical management of renal cortical neoplasms. Arch. Pathol. Lab. Med. 2014 Dec 01 [PMID: 25427045] (IHC-P, Human)

Details:
Carbonic Anhydrase IX/CA9 antibody used for IHC-P on sections of human renal biopsies having sarcomatoid area with focal necrosis and clear cell renal cell carcinoma (Figure 3.).
IHC-P Human
Janmohamed SR, Brinkhuizen T, den Hollander JC et al. Support for the hypoxia theory in the pathogenesis of infantile haemangioma. Clin. Exp. Dermatol. 2014 Dec 16 [PMID: 25511669] (IHC-P, Human) IHC-P Human
Lee H, Yoon C, Park DJ et al. Inhibition of Vascular Endothelial Growth Factor A and Hypoxia-Inducible Factor 1alpha Maximizes the Effects of Radiation in Sarcoma Mouse Models Through Destruction of Tumor Vasculature. Int. J. Radiat. Oncol. Biol. Phys. 2014 Dec 24 [PMID: 25544668]
Kang HJ, Kim IH, Sung CO et al. Expression of carbonic anhydrase 9 is a novel prognostic marker in resectable hepatocellular carcinoma Virchows Arch. 2015 Jan 11 [PMID: 25577552] (IHC-P, Human)

Details:
Carbonic Anhydrase IX/CA9 antibody used at 1:1000 dilution for IHC-P staining on formalin-fixed, paraffin-embedded tissue sections/TMAs created from surgically resected hepatocellular carcinoma/HCC and adjacent non-neoplastic liver tissue - 32 minutes of heat-induced epitope retrieval with Cell Conditioning 1 /CC1 buffer, 16 minutes of primary antibody incubation on Ventana's autoimmunostainer/Bench Mark XT automatic immunostaining device, staining development with Ventana's OptiView DAB IHC Detection Kit. Fig 2a-f documents the staining images showing weak, moderate and strong membranous expression levels of carbonic anhydrase 9 protein and the overall data in this study establishes CA9 protein as a poor prognostic factor in resectable HCC patients.
IHC-P Human
Dulloo I, Phang BH, Othman R et al. Hypoxia-inducible TAp73 supports tumorigenesis by regulating the angiogenic transcriptome. Nat. Cell Biol. 2015 Apr 01 [PMID: 25774835] (IHC-P, Human) IHC-P Human
Cotroneo E, Ashek A, Wang L et al. Iron Homeostasis and Pulmonary Hypertension: Iron Deficiency Leads to Pulmonary Vascular Remodeling in the Rat. Circ. Res. 2015 Mar 12 [PMID: 25767292] (IHC, Rat) IHC Rat
Polanski Radoslaw, Hodgkinson Cassandra L, Fusi Alberto et al. Activity of the monocarboxylate transporter 1 inhibitor AZD3965 in small cell lung cancer. Clin Cancer Res. 2014 Feb 15 [PMID: 24277449] (IHC) IHC
Morifuji Yoshihiro, Onishi Hideya, Iwasaki Hironori et al. Reoxygenation from chronic hypoxia promotes metastatic processes in pancreatic cancer through the Hedgehog signaling. Cancer Sci. 2014 Feb 11 [PMID: 24397700]
Al-Ahmadie HA, Alden D, Qin LX et al. Carbonic anhydrase IX expression in clear cell renal cell carcinoma: an immunohistochemical study comparing 2 antibodies. Am J Surg Pathol 2008 Mar [PMID: 18300814] (IHC, Human) IHC Human
Skapa P, Hyrsl L, Zavada J et al. Carbonic anhydrase IX expression in clear cell renal cell carcinomas negatively correlates with the proportion of the granular cell component. J Clin Oncol 2008 Aug 1 [PMID: 18669473] (Human) Human
Bachmann IM, Ladstein RG, Straume O et al. Tumor necrosis is associated with increased alphavbeta3 integrin expression and poor prognosis in nodular cutaneous melanomas. BMC Cancer 2008 [PMID: 19061491] (IHC, Human) IHC Human
Dumais J. Plant morphogenesis: a role for mechanical signals. Curr Biol 2009 Mar 10 [PMID: 19278636] (IHC, Plant) IHC Plant
Nakao M, Ishii G, Nagai K et al. Prognostic significance of carbonic anhydrase IX expression by cancer-associated fibroblasts in lung adenocarcinoma. Cancer 2009 Jun 15 [PMID: 19365853] (WB, Human) WB Human
Di Napoli A, Signoretti S. Tissue biomarkers in renal cell carcinoma: issues and solutions. Cancer 2009 May 15 [PMID: 19402057] (Human) Human
Hong YS, Cho HJ, Kim SY et al. Carbonic anhydrase 9 is a predictive marker of survival benefit from lower dose of bevacizumab in patients with previously treated metastatic colorectal cancer. BMC Cancer 2009 [PMID: 19619339] (IHC, Human) IHC Human
Zhou GX, Ireland J, Rayman P et al. Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials. Urology 2010 Feb [PMID: 19963243] (ELISA, Human) ELISA Human
Park I, Larson PE, Zierhut ML et al. Hyperpolarized 13C magnetic resonance metabolic imaging: application to brain tumors. Neuro Oncol 2010 Feb [PMID: 20150380] (IHC) IHC
O'Toole D, Couvelard A, Rebours V et al. Molecular markers associated with response to chemotherapy in gastro-entero-pancreatic neuroendocrine tumors. Endocr Relat Cancer 2010 Dec [PMID: 20570957] (IHC, Human) IHC Human
Newton IP, Kenneth NS, Appleton PL et al. Adenomatous polyposis coli and hypoxia-inducible factor-1{alpha} have an antagonistic connection. Mol Biol Cell 2010 Nov 1 [PMID: 20844082] (ChIP) ChIP
Nelson MP, Christmann BS, Werner JL et al. IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina. J Immunol 2011 Feb 15 [PMID: 21220696] (ChIP) ChIP
Chaudary N, Pintilie M, Schwock J et al. Characterization of the Tumor-Microenvironment in Patient-Derived Cervix Xenografts (OCICx). Cancers (Basel) 2012 [PMID: 24213469] (IHC) IHC
Yoon C, Lee HJ, Park DJ et al. Hypoxia-activated chemotherapeutic TH-302 enhances the effects of VEGF-A inhibition and radiation on sarcomas. Br J Cancer 2015 May 26 [PMID: 26010414]
Yoon C, Lee HJ, Park DJ et al. Hypoxia-activated chemotherapeutic TH-302 enhances the effects of VEGF-A inhibition and radiation on sarcomas. Br. J. Cancer. 2015 May 26 [PMID: 26010414] (IHC-P, ICC/IF, Human) ICC/IF, IHC-P Human
Ward C, Meehan J, Mullen P et al. Evaluation of carbonic anhydrase IX as a therapeutic target for inhibition of breast cancer invasion and metastasis using a series of in vitro breast cancer models Oncotarget 2015 Jul 04 [PMID: 26259239]
Heijmen L, Punt CJ, Ter Voert EG et al. Monitoring the effects of bevacizumab beyond progression in a murine colorectal cancer model: a functional imaging approach. Invest New Drugs 013 Jan 17 [PMID: 23325291] (IHC, ICC/IF, Mouse)

Details:
Using the Biotin conjugated version of NB100-417, catalog number NB100-417B.
ICC/IF, IHC Mouse
Heijmen L, Voert EGW, Punt CJA. Monitoring hypoxia and vasculature during bevacizumab treatment in a murine colorectal cancer model. Contrast Media Mol Imaging 2014 May-Jun [PMID: 24700751] (IHC-Fr, Human)

Details:
Using the Biotin conjugated version of NB100-417, catalog number NB100-417B.
IHC-Fr Human
Jamali S, Klier M, Ames S et al. Hypoxia-induced carbonic anhydrase IX facilitates lactate flux in human breast cancer cells by non-catalytic function. Sci Rep 2015 Sep 04 [PMID: 26337752] (WB, ICC/IF, Human) ICC/IF, WB Human
Kinoshita H, Yashiro M, Fukuoka T et al. Diffuse-type Gastric Cancer Cells Switch Their Driver Pathways from FGFR2 Signaling to SDF1/CXCR4 Axis in Hypoxic Tumor Microenvironments. Carcinogenesis 2015 Sep 18 [PMID: 26385890] (IHC-P, Mouse) IHC-P Mouse
Mitsuhashi A, Goto H, Saijo A et al. Fibrocyte-like cells mediate acquired resistance to anti-angiogenic therapy with bevacizumab. Nat Commun. 2015 Dec 04 [PMID: 26635184] (IHC-Fr, Mouse) IHC-Fr Mouse
Scholz A, Harter PN, Cremer S et al. Endothelial cell-derived angiopoietin-2 is a therapeutic target in treatment-naive and bevacizumab-resistant glioblastoma. EMBO Mol Med. 2016 Jan 05 [PMID: 26666269] (IHC-P, Human) IHC-P Human
Song CW, Lee YJ, Griffin RJ et al. Indirect Tumor Cell Death After High-Dose Hypofractionated Irradiation: Implications for Stereotactic Body Radiation Therapy and Stereotactic Radiation Surgery. Int J Radiat Oncol Biol Phys 2015 Sep 1 [PMID: 26279032]
Baine MK, Turcu G, Zito CR et al. Characterization of tumor infiltrating lymphocytes in paired primary and metastatic renal cell carcinoma specimens. Oncotarget 2015 Sep 22 [PMID: 26317902]
Islam SS, Mokhtari RB, Akbari P et al. Simultaneous Targeting of Bladder Tumor Growth, Survival, and Epithelial-to-Mesenchymal Transition with a Novel Therapeutic Combination of Acetazolamide (AZ) and Sulforaphane (SFN). Target Oncol 2015 Oct 10 [PMID: 26453055]

Product General Protocols

View specific protocols for Carbonic Anhydrase IX/CA9 Antibody (NB100-417): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

Ask a Scientist

Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

FAQs for Carbonic Anhydrase IX/CA9 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Customers Who Viewed This Item Also Viewed...

AF-293-NA
Western blot shows lysates of human lung tissue, human kidney tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VEGF at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑293‑NA). The ND50 is typically 0.02-0.12 µg/mL.VEGF was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Goat Polyclonal
Species Human
Applications WB, ICC, Neut

     2 Reviews

28 Publications
NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the HRP conjugate of NB100-105. Detection of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using NB100-105.Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Expression of HIF-1 in intresititial pig cells under 12% of 02 condition. Image from verified customer review.Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, ChIP, ELISA...MORE

     29 Reviews

594 Publications
NB110-39113
Western Blot: Glut1 Antibody [NB110-39113] - GLUT 1 in A549 cells. Image from verified customer review.Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NB110-39113] - GLUT1 antibody was tested in HEK-293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Flow Cytometry: Glut1 Antibody [NB110-39113] - Analysis using the PE conjugate of NB110-39113. Staining of Glut 1 expression on CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. FMO control (red) and isotype control (blue, NBP2-24983) were compared to CD4+ T cells (orange), and this PE conjugated Glut 1 antibody positively stained CD4+ lymphocytes isolated from Mouse (Image submitted by Verified Customer).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ChIP, Flow...MORE

     2 Reviews

12 Publications
NBP2-45283
Immunohistochemistry-Paraffin: ABO, Blood Group A Antigen Antibody (SPM279) [NBP2-45283] - Human Colon Carcinoma stained with Multi Cytokeratin Monoclonal Antibody (SPM583).Immunohistochemistry-Paraffin: ABO, Blood Group A Antigen Antibody (SPM279) [NBP2-45283] - Human Colon Carcinoma stained with Lewis A Monoclonal Antibody (SPM279).

Mouse Monoclonal
Species Human, Mouse
Applications Flow, IHC-P, IF

MAB602
IL‑2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Mouse Anti-Human/Primate IL‑2 Monoclonal Antibody (Catalog # MAB602) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Mouse Monoclonal
Species Human, Primate
Applications ICC

9 Publications
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, ELISA, Flow...MORE

     2 Reviews

19 Publications
NB100-485
Western Blot: Von Hippel Lindau Antibody [NB100-485] - Detection of VHL in human kidney samples (30 ug)using NB 100-485. ECL detection 10 seconds. Lane 1: 0.5 ug/ml primary Lane 2: 2 ug/ml primary

Rabbit Polyclonal
Species Human
Applications WB, IP

     1 Review

3 Publications
NBP1-89843
Immunocytochemistry/Immunofluorescence: HYPB Antibody [NBP1-89843] - Staining of human cell line A-431 shows positivity in nucleus & cytoplasm.Immunohistochemistry-Paraffin: HYPB Antibody [NBP1-89843] - Staining of human oral mucosa shows moderate nuclear positivity in squamous epithelial cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

1 Publication
NBP1-19371
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse spleen using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong signal in the membranes of a subset of cells in the spleen section and the staining showed punctate appearance in some cells.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, Simple Western, ICC/IF...MORE

     5 Reviews

7 Publications
NBP2-03008
Western Blot: Haptoglobin Antibody (4H5) [NBP2-03008] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Haptoglobin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Haptoglobin.Immunocytochemistry/Immunofluorescence: Haptoglobin Antibody (4H5) [NBP2-03008] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Haptoglobin.Flow Cytometry: Haptoglobin Antibody (4H5) [NBP2-03008] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Haptoglobin antibody, and then analyzed by flow cytometry.

Mouse Monoclonal
Species Human, Monkey
Applications WB, Flow, ICC/IF

AF231
A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EGF R/ErbB1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF231, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).    Western  blot shows lysates of HeLa human cervical epithelial carcinoma cell line and  MDA‑MB‑231 human breast cancer cell line. PVDF membrane  was probed with 1 µg/mL of Goat Anti-Human EGF R/ErbB1  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017).  A specific band was detected for EGF R/ErbB1 at approximately  175 kDa (as indicated). This experiment was conducted under reducing  conditions and using Immunoblot  Buffer Group 1.

Goat Polyclonal
Species Human
Applications WB, Flow, IP

5 Publications
MAB610
TNF‑ alpha  was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human TNF‑ alpha  Monoclonal Antibody (Catalog # MAB610) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Recombinant Human TNF‑ alpha  (Catalog # 210‑TA) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha  (0.75 ng/mL) is neutral­ized (green line) by increasing concentrations of Mouse Anti-Human TNF‑ alpha  Monoclonal Antibody (Catalog # MAB610). The ND50 is typically 0.01‑0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Mouse Monoclonal
Species Human
Applications WB, ICC, Neut

26 Publications
NBP2-24915
Western Blot: Superoxide Dismutase 1 Antibody [NBP2-24915] - Analysis of SOD in liver lysate of 1) human, 2) mouse and 3) rat using this antibody. 025 ug/ml.Immunohistochemistry-Paraffin: Superoxide Dismutase 1 Antibody [NBP2-24915] - Analysis of Superoxide Dismutase 1 in paraffin-embedded formalin-fixed human liver tissue using an isotype control (top) and NBP2-24915 (bottom) at 5 ug/ml.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, IHC-P

2 Publications
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, IHC, IHC-P

4 Publications
MAB1228
  Glycophorin A  was detected in immersion fixed paraffin-embedded sections of human lung  using Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog #  MAB1228) at 1.7 µg/mL overnight at 4 °C.  Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining  Kit (brown; Catalog # CTS002)  and counterstained with hematoxylin (blue). Specific staining was localized  to cell membranes of red blood cells. View our protocol for Chromogenic  IHC Staining of Paraffin-embedded Tissue Sections.  TF‑1  human erythroleukemic cell line was stained with Mouse Anti-Human  Glycophorin A Monoclonal Antibody (Catalog # MAB1228, filled  histogram) or isotype control antibody (Catalog # MAB002,  open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG  Secondary Antibody (Catalog # F0102B).

Mouse Monoclonal
Species Human
Applications Flow, IHC, IP

2 Publications
NBP1-88116
Immunocytochemistry/Immunofluorescence: Ceruloplasmin Antibody [NBP1-88116] - Staining of human cell line A-431 shows positivity in cytoplasm.Immunohistochemistry-Paraffin: Ceruloplasmin Antibody [NBP1-88116] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

2 Publications
NB600-326
Western Blot: GST Epitope Tag Antibody [NB600-326] - Analysis using the Biotin conjugate of NB600-326. Detection of 0.1, 0.3, or 1.0 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000). Detection: Streptavidin-HRP (0.1 ug/ml) and chemiluminescence with an exposure time of 60 seconds.Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).Western Blot: GST Epitope Tag Antibody [NB600-326] - Analysis using the HRP conjugate of NB600-326. Detection of 1.0, 0.3, or 0.1 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

Rabbit Polyclonal
Species Human, Non-species specific
Applications WB, Simple Western, ELISA...MORE

     2 Reviews

9 Publications
MAB206
Recombinant Human IL‑6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human/Primate IL‑6 Monoclonal Antibody (Catalog # MAB206). The ND50 is typically 0.05-0.15 µg/mL.

Mouse Monoclonal
Species Human, Primate
Applications WB, Neut

31 Publications
NB100-64849

Mouse Monoclonal
Species Human
Applications Flow, IHC, IHC-Fr...MORE

     1 Review

Bioinformatics Tool for Carbonic Anhydrase IX/CA9

Discover related pathways, diseases and genes to Carbonic Anhydrase IX/CA9. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool
Vizit™, under license from BioVista Inc.

Research Areas for Carbonic Anhydrase IX/CA9

Find related products by research area.

Blogs on Carbonic Anhydrase IX/CA9

Check out the latest blog posts on Carbonic Anhydrase IX/CA9.
Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e...  Read full blog post.