Carbonic Anhydrase IX/CA9 Antibody 0.1 ml Hypoxia Marker

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Western Blot: Carbonic Anhydrase IX Antibody [NB100-417] - Carbonic Anhydrase IX on various human cell lysates. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX Antibody [NB100-417] - Immunofluorescence of human RCC tumor cryosections using NB100-417 (Panel A). Panel B shows staining with normal rabbit serum.
Immunohistochemistry-Frozen: Carbonic Anhydrase IX Antibody [NB100-417] - Analysis of human colon carcinoma, xenografted in mice. Image courtesy of an anonymous product review.
Western Blot: Carbonic Anhydrase IX Antibody [NB100-417] - Analysis of Carbonic Anhydrase IX expression in 1) HeLa, 2) MDA-MB-231 and 3) A549 whole cell lysates.
Western Blot: Carbonic Anhydrase IX Antibody [NB100-417] - Detection of carbonic anhydrase IX in transfected HEK cell lysate using NB 100-417. Rabbit IgG was used as a negative control.
Western Blot: Carbonic Anhydrase IX Antibody [NB100-417] - Analysis of CAIX on rat renal cortex using NB100-417.
Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX Antibody [NB100-417] - Immunofluorescence of HEK 293 cells using NB100-417. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock transfected cells.
Immunohistochemistry: Carbonic Anhydrase IX Antibody [NB100-417] - Renal carcinoma tissue stained with polyclonal carbonic anhydrase IX.
Simple Western: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Simple Western lane view shows a specific band for CAIX in 0.1 mg/ml of HeLa lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Carbonic Anhydrase IX/CA9 Antibody Summary

Species Human, Mouse, Rat, Canine, Plant
Tested Applications WB, Simple Western, ELISA, GS, ICC/IF, IHC, IHC-Fr, IHC-P, ChIP
Immunogen affinity purified
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Carbonic Anhydrase IX/CA9 Antibody Details

Hypoxia Marker
A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-500) [UniProt Q16790]

Species Reactivity

Human, dog, rat and mouse (PMID 22842475). Plant reactivity reported in scientific literature (PMID: 19278636)
Reviewed Species Read 3 rated 4.7
using NB100-417 in the following species:

Publications Read Publications using NB100-417 in the following species:


  • Western Blot 1:2000
  • Simple Western 1:50
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
This Carbonic Anhydrase IX antibody is useful for Western blot, Immunofluorescence/Immunocytochemistry, and Immunohistochemistry. Gel Super Shift Assays was reported in scientific literature. In Western blot a band is observed ~53 kDa.In Simple Western only 10-15 uL of the recommended dilution is used per data point. Use in ELISA reported in scientific literature (PMID 19963243). Use in chromatin immunoprecipitation reported in multiple pieces of scientific literature.
Molecular Weight
55 kDa
Positive Controls
Other Available
Alexa Fluor (R) 405 Labeled NB100-417AF405
Alexa Fluor (R) 488 Labeled NB100-417AF488
Alexa Fluor (R) 647 Labeled NB100-417AF647
Alexa Fluor (R) 700 Labeled NB100-417AF700
Biotin Labeled NB100-417B
DyLight 405 Labeled NB100-417V
DyLight 488 Labeled NB100-417G
DyLight 550 Labeled NB100-417R
DyLight 650 Labeled NB100-417C
FITC Labeled NB100-417F
HRP Labeled NB100-417H
Control Peptide
Carbonic Anhydrase IX/CA9 Peptide (NB100-417PEP)
Reviewed Applications
Read 3 Review rated 4.7
NB100-417 in the following applications:

Read Publications using
NB100-417 in the following applications:

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Average Rating: 4.7
Recent Reviews
WB Tested In Human
Reviewed On: 07/21/2014
Rated: 5

Carbonic Anhydrase IX/CA9 NB100-417
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Carbonic Anhydrase IX/CA9 Antibody (NB100-417) has been mentioned in at least 98 publications.
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Unit Size
0.1 ml
1.0 mg/ml
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.


Gene Symbol CA9

Alternate Names for Carbonic Anhydrase IX/CA9 Antibody

  • CA9
  • G250
  • MN
  • RCC
  • CA-IX
  • CAIXcarbonic anhydrase 9
  • Carbonate dehydratase IX
  • carbonic anhydrase IXpMW1
  • carbonic dehydratase
  • EC
  • G250
  • Membrane antigen MN
  • MNRCC-associated antigen G250
  • P54/58N
  • RCC-associated protein G250
  • Renal cell carcinoma-associated antigen G250
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Related Products by Gene


Carbonic anhydrase IX (CAIX) is a member of the Carbonic anhydrase family which are enzymes that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. They are abundant in all mammalian tissues and because of their functionality, they have become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). Carbonic Anhydrase IX (CA IX) is one of the most hypoxically inducible genes because of its stability and the location of the expressed protein within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX (CA9) results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

Reviews for Carbonic Anhydrase IX/CA9 Antibody (NB100-417) (3) 4.73

Read what people are saying who have used Carbonic Anhydrase IX/CA9 Antibody (NB100-417).

We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2: Western Blot, Immunocytochemistry.
Average Rating: 4.7
(Based on 3 reviews)

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Images Ratings Applications Species Reviewed By Date Details
WB Human Anonymous 12/10/2014


ApplicationWestern Blot
Sample TestedA2780 ovarian cancer cells


Blocking Details5% BSA


Detection NotesECL
Western Blot Carbonic Anhydrase IX/CA9 NB100-417
Click to Enlarge
WB Human Anonymous 07/21/2014


ApplicationWestern Blot
Sample Tested20 ug whole cell lysate
Sample PretreatmentNo treatment


Blocking Details1xPBST with 5% non-fat milk, RT 1 hour

Primary Anitbody

Dilution Ratio1xpBST with 5% non-fat milk, overnight 4C, 1:1000 dilution

Secondary Antibody

Secondary Manufacturer Cat#sc-2004
Secondary Concentration1:2000 dilution


Detection NotesECL, exposured for 3 min
Immunocytochemistry Carbonic Anhydrase IX/CA9 NB100-417
Click to Enlarge
Very Good
ICC Human Anonymous 12/21/2011


Sample Testedhuman cells
Sample Pretreatmentuntreated
CommentsReally nice results using microscopy.


Blocking DetailsBlocking Buffer: 1% Donkey serum PBS, Blocking Time: 30 minutes, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: PBS/1% Donkey serum, Dilution Ratio: 1:250, Incubation Time: 1 hour, Incubation Temp: 20 C


Detection NotesDetection Visualization Method: fluorescence at 488, Negative Control: secondary and primary only
Fixation DetailsSample Preparation: PFA fixed

Publications for Carbonic Anhydrase IX/CA9 Antibody (NB100-417) (98)

Read what people are writing who have used Carbonic Anhydrase IX/CA9 Antibody (NB100-417).

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Plant.

We have publications tested in 7 applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P.

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Sathornsumetee S, Cao Y, Marcello JE et al. Tumor angiogenic and hypoxic profiles predict radiographic response and survival in malignant astrocytoma patients treated with bevacizumab and irinotecan. J Clin Oncol;26(2):271-8. 2008 Jan 10. [PMID: 1818266] (IHC-P, Human) IHC-P Human
Atkins M, Regan M, McDermott D et al. Carbonic anhydrase IX expression predicts outcome of interleukin 2 therapy for renal cancer. Clin Cancer Res;11(10):3714-21. 2005 May 15. [PMID: 15897568] (IHC, Human) IHC Human
Van den Eynden GG, Van der Auwera I, Van Laere SJ et al. Angiogenesis and hypoxia in lymph node metastases is predicted by the angiogenesis and hypoxia in the primary tumour in patients with breast cancer. Br J Cancer;93(10):1128-36. 2005 Nov 14. [PMID: 16251878] (IHC-P, Human) IHC-P Human
Chamboredon S, Ciais D, Desroches-Castan A et al. Hypoxia-inducible Factor-1{alpha} mRNA: a new target for destabilization by tristetraprolin in endothelial cells. Mol Biol Cell. 2011 Jul 20. [PMID: 21775632] (WB, Human) WB Human
Garcia-Parra R, Wood D, Shah RB et al. Investigation on tumor hypoxia in resectable primary prostate cancer as demonstrated by (18)F-FAZA PET/CT utilizing multimodality fusion techniques. Eur J Nucl Med Mol Imaging. 2011 Jul 16. [PMID: 21833840] (IHC-P, Human) IHC-P Human
Ogino T, Onishi H, Suzuki H et al. Inclusive estimation of complex antigen presentation functions of monocyte-derived dendritic cells differentiated under normoxia and hypoxia conditions. Cancer Immunology, Immunotherapy: CII. 2011 Sep 20. [PMID: 21932134]
Deschamps L, Bacha D, Rebours V, Mebarki M, Bretagnol F, Panis Y, Bedossa P, Ruszniewski P, Couvelard A. The Expression of the Hypoxia Markers CA9 and CXCR4 Is Correlated with Survival in Patients with Neuroendocrine Tumours of the Ileum. Neuroendocrinology. 2011 Nov 29. [PMID: 22133596] (IHC, Human) IHC Human
Matsumura Y, Ishii G, Aokage K, Kuwata T, Hishida T, Yoshida J, Nishimura M, Nagai K, Ochiai A. Morphophenotypic characteristics of intralymphatic cancer and stromal cells susceptible to lymphogenic metastasis. Cancer Sci. 2012 Mar 16. [PMID: 22429811] (IHC, Human) IHC Human
Speisky D, Duces A, Bieche I et al. Molecular Profiling of Pancreatic Neuroendocrine Tumors in Sporadic and Von Hippel-Lindau Patients. Clin Cancer Res;18(10):2838-2849. 2012 May 15. [PMID: 22461457] (IHC, Human) IHC Human
Pellegrini L, Pucci B, Villanova L, Marino ML, Marfe G, Sansone L, Vernucci E, Bellizzi D, Reali V, Fini M, Russo MA, Tafani M. SIRT3 protects from hypoxia and staurosporine-mediated cell death by maintaining mitochondrial membrane potential and intracellular pH. Cell Death Differ. 2012 May 18. [PMID: 22595756] (WB, Human) WB Human
Muriel Lopez C, Esteban E, Astudillo A, Pardo P, Berros JP, Izquierdo M, Crespo G, Fonseca PJ, Sanmamed M, Martinez-Camblor P. Predictive factors for response to treatment in patients with advanced renal cell carcinoma. Invest New Drugs. 2012 May 27. [PMID: 22644070] (IHC, Human) IHC Human
Melvin A, Mudie S, Rocha S. The chromatin remodeler ISWI regulates the cellular response to hypoxia, role of FIH. Mol Biol Cell. 2011 Sep 7. [PMID: 21900490] (Human) Human
Yoo H, Baia GS, Smith JS et al. Expression of the hypoxia marker carbonic anhydrase 9 is associated with anaplastic phenotypes in meningiomas. Clin Cancer Res;13(1):68-75. 2007 Jan 1. [PMID: 17200340] (IHC-P, Human) IHC-P Human
Li Y, Wang H, Tu C et al. Role of hypoxia and EGF on expression, activity, localization and phosphorylation of carbonic anhydrase IX in MDA-MB-231 breast cancer cells. Biochim Biophys Acta. 2010 Oct 1. [PMID: 20920536] (WB, Human) WB Human
Guise CP, Abbattista MR, Tipparaju SR et al. Diflavin Oxidoreductases Activate the Bioreductive Prodrug PR104A Under Hypoxia. Molecular Pharmacology. 2011 Oct 7. [PMID: 21984255]
Onishi H, Morifuji Y, Kai M, Suyama K, Iwasaki H, Katano M. Hedgehog inhibitor decreases chemosensitivity to 5-fluorouracil and gemcitabine under hypoxic conditions in pancreatic cancer. Cancer Sci. 2012 Apr 6. [PMID: 22486854] (IHC, ICC/IF, Mouse) ICC/IF, IHC Mouse
Al-Ahmadie HA, Alden D, Fine SW et al. Role of immunohistochemistry in the evaluation of needle core biopsies in adult renal cortical tumors: an ex vivo study. Am. J. Surg. Pathol. 2011 Jul. [PMID: 21677535] (IHC, Human) IHC Human
Westbury CB, Pearson A, Nerurkar A et al. Hypoxia can be detected in irradiated normal human tissue: a study using the hypoxic marker pimonidazole hydrochloride. Br J Radiol;80(959):934-8. 2007 Nov. [PMID: 17908818] (IHC-P, Human) IHC-P Human
Kaidi A, Moorghen M, Williams AC et al. Is the downregulation of EphB2 receptor expression during colorectal tumorigenesis due to hypoxia?. Gut 56(11):1637-8. 2007 Nov. [PMID: 17938437] (IHC-P, Human) IHC-P Human
Sakata K, Someya M, Nagakura H et al. Brachytherapy for oral tongue cancer: an analysis of treatment results with various biological markers. Jpn J Clin Oncol;38(6):402-7. 2008 Jun. [PMID: 18573850] (IHC-P, Human) IHC-P Human
Lapinski JE, Chen L, Zhou M. Distinguishing clear cell renal cell carcinoma, retroperitoneal paraganglioma, and adrenal cortical lesions on limited biopsy material: utility of immunohistochemical markers. Appl Immunohistochem Mol Morphol;18(5):414-21. 2010 Oct. [PMID: 20861762] (IHC, Human) IHC Human
Mackenzie KA, Miller AP, Hock BD, Gardner J, Simcock JW, Roake JA, Dachs GU, Robinson BA, Currie MJ. Angiogenesis and host immune response contribute to the aggressive character of non-melanoma skin cancers in renal transplant recipients. Histopathology;58(6):875-85. 2011 May. [PMID: 21585427] (IHC, Human) IHC Human
Jung JH, Kim HJ, Yeom J, Yoo C, Shin J, Yoo J, Kang CS, Lee C. Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer. J Gastroenterol;47(1):37-48. 2012 Jan. [PMID: 22015694] (WB, Human) WB Human
Lim CS, Kiriakidis S, Paleolog EM, Davies AH. Increased activation of the hypoxia-inducible factor pathway in varicose veins. J Vasc Surg;55(5):1427-1439e1. 2012 May. [PMID: 22277691] (WB, Human) WB Human
Banuelos CA, Banath JP, Kim J-Y et al. {gamma}H2AX Expression in Tumors Exposed to Cisplatin Fractionated Irradiation. Clin Cancer Res;15(10):3344-3353. 2009 [PMID: 19401347] (IHC-P, Human) IHC-P Human
Chen J, Kobayashi M, Darmanin S et al. Hypoxia-Mediated Up-Regulation of Pim-1 Contributes to Solid Tumor Formation. Am J Pathol;175(1):400-411. 2009 [PMID: 19528349] (IHC-P, Mouse) IHC-P Mouse
Loinard C, Ginouves A, Vilar J et al. Inhibition of Prolyl Hydroxylase Domain Proteins Promotes Therapeutic Revascularization. Circulation;120(1):50-59. 2009 [PMID: 19546390] (WB, Human, Mouse) WB Human, Mouse
Lucio-Eterovic AK, Piao Y, de Groot JF. Mediators of Glioblastoma Resistance Invasion during Antivascular Endothelial Growth Factor Therapy. Clin Cancer Res:1078-0432.CCR-1009-0575. 2009 [PMID: 19567589] (IHC-P, Human) IHC-P Human
Benito J, Shi Y, Szymanska B et al. Pronounced Hypoxia in Models of Murine and Human Leukemia: High Efficacy of Hypoxia-Activated Prodrug PR-104. PLoS ONE. 2011 [PMID: 21853076]
Narita T, Yin S, Gelin CF et al. Identification of a Novel Small Molecule HIF-1{alpha} Translation Inhibitor. Clin Cancer Res 9;15(19):6128-6136. 200 [PMID: 19789328] (WB, Human) WB Human
Chaumeil MM, Ozawa T, Park I et al. Hyperpolarized 13C MR spectroscopic imaging can be used to monitor Everolimus treatment in vivo in an orthotopic rodent model of glioblastoma. NeuroImage. [PMID: 21807103] (IHC-P, Rat) IHC-P Rat
Wang Y, Roche O, Xu C et al. Hypoxia promotes ligand-independent EGF receptor signaling via hypoxia-inducible factor-mediated upregulation of caveolin-1 Proc Natl Acad Sci U S A 2012 Mar 12 [PMID: 22411794] (EMSA, WB, Human) WB Human
Eguchi D, Ikenaga N, Ohuchida K et al. Hypoxia enhances the interaction between pancreatic stellate cells and cancer cells via increased secretion of connective tissue growth factor J Surg Res 2012 Jul 6 [PMID: 22795353] (IHC, Human) IHC Human
Fromy B, Lingueglia E, Sigaudo-Roussel D et al. Asic3 is a neuronal mechanosensor for pressure-induced vasodilation that protects against pressure ulcers Nat Med 2012 Jul 29 [PMID: 22842475] (IHC, Mouse) IHC Mouse
Engler JR, Robinson AE, Smirnov I et al. Increased microglia/macrophage gene expression in a subset of adult and pediatric astrocytomas PLoS One 2012 [PMID: 22937035] (IHC, Human) IHC Human
Weber DC, Tille JC, Combescure C et al. The prognostic value of expression of HIF1alpha, EGFR and VEGF-A, in localized prostate cancer for intermediate- and high-risk patients treated with radiation therapy with or without androgen deprivation therapy Radiat Oncol 2012 Apr 30 [PMID: 22546016] (IHC, Human) IHC Human
Brouillet S, Murthi P, Hoffmann P et al. EG-VEGF controls placental growth and survival in normal and pathological pregnancies: case of fetal growth restriction (FGR) Cell Mol Life Sci 2012 Sep 2 [PMID: 22941044] (IHC, Human) IHC Human
Muriel Lopez C, Esteban E, Berros JP et al. Prognostic Factors in Patients With Advanced Renal Cell Carcinoma Clin Genitourin Cancer 2012 Sep 6 [PMID: 22959659] (IHC, Human) IHC Human
Piao Y, Liang J, Holmes L et al. Glioblastoma resistance to anti-VEGF therapy is associated with myeloid cell infiltration, stem cell accumulation, and a mesenchymal phenotype Neuro Oncol 2012 Sep 10 [PMID: 22965162] (IHC, Human, Mouse) IHC Human, Mouse
Currie MJ, Beardsley BE, Harris GC et al. Immunohistochemical analysis of cancer stem cell markers in invasive breast carcinoma and associated ductal carcinoma in situ: relationships with markers of tumor hypoxia and microvascularity Hum Pathol 2012 Oct 1 [PMID: 23036368] (IHC, Human) IHC Human
Proescholdt MA, Merrill MJ, Stoerr EM et al. Function of carbonic anhydrase IX in glioblastoma multiforme Neuro Oncol 2012 Nov [PMID: 23074198] (IHC, WB, Human) IHC, WB Human
Kato Y, Yashiro M, Noda S et al. Expression of a hypoxia-associated protein, carbonic anhydrase-9, correlates with malignant phenotypes of gastric carcinoma. Digestion 2010 [PMID: 20588040] (IHC, Human) IHC Human
Arvidsson Y, Bergstrom A, Arvidsson L et al. Hypoxia stimulates CXCR4 signalling in ileal carcinoids. Endocr Relat Cancer 2010 Jun 1 [PMID: 20071457] (IHC, Human) IHC Human
Pinato DJ, Ramachandran R, Toussi ST et al. Immunohistochemical markers of the hypoxic response can identify malignancy in phaeochromocytomas and paragangliomas and optimize the detection of tumours with VHL germline mutations Br J Cancer 2012 Dec 20 [PMID: 23257898] (IHC, Human) IHC Human
Delhaas T, Van der Meer SF, Schaart G et al. Steep Increase in Myonuclear Domain Size During Infancy Anat Rec (Hoboken) 2012 Dec 4 [PMID: 23213045] (IHC, ICC/IF, Human) ICC/IF, IHC Human
Yin S, Kaluz S, Devi NS et al. Arylsulfonamide KCN1 Inhibits In Vivo Glioma Growth and Interferes with HIF Signaling by Disrupting HIF-1alpha Interaction with Cofactors p300/CBP Clin Cancer Res 2012 Dec 15 [PMID: 22923450] (WB, Human) WB Human
Zhang Q, Bai X, Chen W et al. Wnt/beta-catenin signaling enhances hypoxia-induced epithelial-mesenchymal transition in hepatocellular carcinoma via crosstalk with hif-1alpha signaling. Carcinogenesis 2013 Jan 27 [PMID: 23358852] (WB, Human) WB Human
Aarntzen EH, Srinivas M, Bonetto F et al. Targeting of 111In-Labeled Dendritic Cell Human Vaccines Improved by Reducing Number of Cells. Clin Cancer Res 2013 Mar 15 [PMID: 23382117] (IHC, Human) IHC Human
Cho SY, Lee HJ, Lee HJ et al. Activation of AMP-Activated Protein Kinase alpha and Extracelluar Signal-Regulated Kinase Mediates CB-PIC-Induced Apoptosis in Hypoxic SW620 Colorectal Cancer Cells Evid Based Complement Alternat Med 2013 [PMID: 23589723] (IHC-P, Human) IHC-P Human
Li Y, Wang H, Oosterwijk E et al. Antibody-specific detection of CAIX in breast and prostate cancers. Biochem Biophys Res Commun 2009 Aug 28 [PMID: 19538935] (IHC-P, Human) IHC-P Human
Carbonell WS, Delay M, Jahangiri A et al. beta1 Integrin Targeting Potentiates Antiangiogenic Therapy and Inhibits the Growth of Bevacizumab-Resistant Glioblastoma. Cancer Res 2013 May 15 [PMID: 23644530] (IHC-P, ICC/IF, Human) ICC/IF, IHC-P Human
Baia GS, Dinca EB, Ozawa T et al. An orthotopic skull base model of malignant meningioma. Brain Pathol 2008 Apr [PMID: 18093250] (IHC-P, Mouse) IHC-P Mouse
Gauter-Fleckenstein B, Fleckenstein K, Owzar K et al. Comparison of two Mn porphyrin-based mimics of superoxide dismutase in pulmonary radioprotection. Free Radic Biol Med 2008 Mar 15 [PMID: 18082148] (IHC-P, Rat) IHC-P Rat
Bussolati B, Lauritano C, Moggio A et al. Renal CD133+/CD73+ Progenitors Produce Erythropoietin under Hypoxia and Prolyl Hydroxylase Inhibition. J Am Soc Nephrol 2013 May 9 [PMID: 23661806]
Campanile C, Arlt MJ, Kramer SD et al. Characterization of Different Osteosarcoma Phenotypes by PET Imaging in Preclinical Animal Models. J Nucl Med 2013 Jun 25 [PMID: 23801674]
Kumar S, Mokhtari RB, Oliveira ID et al. Tumor Dynamics in Response to Antiangiogenic Therapy with Oral Metronomic Topotecan and Pazopanib in Neuroblastoma Xenografts. Transl Oncol 2013 Aug 1 [PMID: 23908692] (IHC, Human) IHC Human
Pistollato F, Abbadi S, Rampazzo E et al. Intratumoral hypoxic gradient drives stem cells distribution and MGMT expression in glioblastoma. Stem Cells 2010 May [PMID: 20309962] (IHC-P, Human) IHC-P Human
Pistollato F, Rampazzo E, Persano L et al. Interaction of hypoxia-inducible factor-1a and Notch signaling regulates medulloblastoma precursor proliferation and fate. Stem Cells 2010 Nov [PMID: 20827750] (IHC-P, Human) IHC-P Human
Dubois L, Peeters SG, van Kuijk SJ et al. Targeting carbonic anhydrase IX by nitroimidazole based sulfamides enhances the therapeutic effect of tumor irradiation: A new concept of dual targeting drugs. Radiother Oncol 2013 Jul 9 [PMID: 23849171] (IHC-Fr, Mouse) IHC-Fr Mouse
Park I, Hu S, Bok R et al. Evaluation of heterogeneous metabolic profile in an orthotopic Human glioblastoma xenograft model using compressed sensing hyperpolarized 3D 13C magnetic resonance spectroscopic imaging. Magn Reson Med 2013 Jul [PMID: 22851374] (IHC-P, Rat) IHC-P Rat
Albert S, Hourseau M, Halimi C et al. Prognostic value of the chemokine receptor CXCR4 and epithelial-to-mesenchymal transition in patients with squamous cell carcinoma of the mobile tongue. Oral Oncol 2012 Dec [PMID: 22776129]
Cioffi S, Martucciello S, Fulcoli FG et al. Tbx1 regulates brain vascularization. Hum Mol Genet. 2013 Sep 7 [PMID: 23945394] (IHC-Fr, ICC/IF, Mouse) ICC/IF, IHC-Fr Mouse
Fahling M, Mathia S, Paliege A et al. Tubular von Hippel-Lindau Knockout Protects against Rhabdomyolysis-Induced AKI. J Am Soc Nephrol. 2013 Aug 22 [PMID: 23970125]
Zhang M, Boughton P, Rose B et al. The Use of Porous Scaffold as a Tumor Model. Int J Biomater. 2013 [PMID: 24101930] (IHC, Human) IHC Human
Wen YA, Stevens PD, Gasser ML et al. Downregulation of PHLPP Expression Contributes to Hypoxia-Induced Resistance to Chemotherapy in Colon Cancer Cells. Mol Cell Biol. 2013 Nov [PMID: 24061475] (WB, Human) WB Human
El-Heliebi A, Kroneis T, Zohrer E et al. Are morphological criteria sufficient for the identification of circulating tumor cells in renal cancer?. J Transl Med. 2013 Sep 17 [PMID: 24044779] (ICC/IF, Human) ICC/IF Human
Pinato DJ, Tan TM, Toussi ST et al. An expression signature of the angiogenic response in gastrointestinal neuroendocrine tumours: correlation with tumour phenotype and survival outcomes. Br J Cancer. 2013 Nov 14 [PMID: 24231952] (IHC-P, Human) IHC-P Human
Chen X, Iliopoulos D, Zhang Q et al. XBP1 promotes triple-negative breast cancer by controlling the HIF1a pathway. Nature 4/3/2014 [PMID: 24670641] (IHC-P, Human) IHC-P Human
Kwon OJ, Park JJ, Ko GH et al. HIF-1a and CA-IX as predictors of locoregional control for determining the optimal treatment modality for early-stage laryngeal carcinoma. Head Neck 2/12/2014 [PMID: 24677746] (IHC-P, Human) IHC-P Human
Sadlecki P, Bodnar M, Grabiec M. The Role of Hypoxia-Inducible Factor-1a, Glucose Transporter-1,(GLUT-1) and Carbon Anhydrase IX in Endometrial Cancer Patients. BioMed Research International 3/12/2014 [PMID: 24745019] (IHC-P, Human) IHC-P Human
Motzer RJ, Hutson TE, Hudes GR et al. Investigation of novel circulating proteins, germ line single-nucleotide polymorphisms, and molecular tumor markers as potential efficacy biomarkers of first-line sunitinib therapy for advanced renal cell carcinoma. Cancer Chemother. Pharmacol. 2014 Aug 07 [PMID: 25100134] (IHC-P, Human)

Rabbit polyclonal Carbonic Anhydrase IX/CA-IX antibody used for IHC on formalin-fixed paraffin-embedded (FFPE) blocks or unstained slides from tumor specimens.
IHC-P Human
Chen LQ, Howison CM, Spier C et al. Assessment of carbonic anhydrase IX expression and extracellular pH in B-cell lymphoma cell line models. Leuk. Lymphoma. 2014 Aug 17 [PMID: 25130478]
Hwa JS, Kwon OJ, Park JJ et al. The prognostic value of immunohistochemical markers for oral tongue squamous cell carcinoma. Eur Arch Otorhinolaryngol. 2014 Aug 29 [PMID: 25169079]
Santos Cd, Tijeras-Raballand A, Serova M et al. Effects of preset sequential administrations of sunitinib and everolimus on tumour differentiation in Caki-1 renal cell carcinoma. Br. J. Cancer. 2014 Nov 25 [PMID: 25422908] (IHC-P, Human) IHC-P Human
Lin XY, Zhang H, Tang N et al. Expression and diagnostic implications of carbonic anhydrase IX in several tumours with predominantly clear cell morphology. Histopathology. 2014 Nov 27 [PMID: 25431204] (IHC-P, Human)

Carbonic Anhydrase IX/CA9 antibody used for IHC-P on sections of clear cell renal cell carcinoma (ccRCC; primary and metastatic) and several other type of tumors with predominantly clear cell morphology - tissues previously fixed in 10% neutral buffered formalin and embedded in paraffin, 4um sections, routine IHC-P processing with streptavidin-peroxidase detection system. ccRCC, and breast / pulmonary/hepatic clear cell carcinoma, urothelial carcinoma with clear cell change, clear cell meningioma/ependymoma, haemangioblastoma, and clear cell hidradenoma showed expression of CAIX. See full text for staining images and the expression levels.
IHC-P Human
Gellert LL, Mehra R, Chen YB et al. The diagnostic accuracy of percutaneous renal needle core biopsy and its potential impact on the clinical management of renal cortical neoplasms. Arch. Pathol. Lab. Med. 2014 Dec 01 [PMID: 25427045] (IHC-P, Human)

Carbonic Anhydrase IX/CA9 antibody used for IHC-P on sections of human renal biopsies having sarcomatoid area with focal necrosis and clear cell renal cell carcinoma (Figure 3.).
IHC-P Human
Janmohamed SR, Brinkhuizen T, den Hollander JC et al. Support for the hypoxia theory in the pathogenesis of infantile haemangioma. Clin. Exp. Dermatol. 2014 Dec 16 [PMID: 25511669] (IHC-P, Human) IHC-P Human
Lee H, Yoon C, Park DJ et al. Inhibition of Vascular Endothelial Growth Factor A and Hypoxia-Inducible Factor 1alpha Maximizes the Effects of Radiation in Sarcoma Mouse Models Through Destruction of Tumor Vasculature. Int. J. Radiat. Oncol. Biol. Phys. 2014 Dec 24 [PMID: 25544668]
Kang HJ, Kim IH, Sung CO et al. Expression of carbonic anhydrase 9 is a novel prognostic marker in resectable hepatocellular carcinoma Virchows Arch. 2015 Jan 11 [PMID: 25577552] (IHC-P, Human)

Carbonic Anhydrase IX/CA9 antibody used at 1:1000 dilution for IHC-P staining on formalin-fixed, paraffin-embedded tissue sections/TMAs created from surgically resected hepatocellular carcinoma/HCC and adjacent non-neoplastic liver tissue - 32 minutes of heat-induced epitope retrieval with Cell Conditioning 1 /CC1 buffer, 16 minutes of primary antibody incubation on Ventana's autoimmunostainer/Bench Mark XT automatic immunostaining device, staining development with Ventana's OptiView DAB IHC Detection Kit. Fig 2a-f documents the staining images showing weak, moderate and strong membranous expression levels of carbonic anhydrase 9 protein and the overall data in this study establishes CA9 protein as a poor prognostic factor in resectable HCC patients.
IHC-P Human
Dulloo I, Phang BH, Othman R et al. Hypoxia-inducible TAp73 supports tumorigenesis by regulating the angiogenic transcriptome. Nat. Cell Biol. 2015 Apr 01 [PMID: 25774835] (IHC-P, Human) IHC-P Human
Cotroneo E, Ashek A, Wang L et al. Iron Homeostasis and Pulmonary Hypertension: Iron Deficiency Leads to Pulmonary Vascular Remodeling in the Rat. Circ. Res. 2015 Mar 12 [PMID: 25767292] (IHC, Rat) IHC Rat
Polanski Radoslaw, Hodgkinson Cassandra L, Fusi Alberto et al. Activity of the monocarboxylate transporter 1 inhibitor AZD3965 in small cell lung cancer. Clin Cancer Res. 2014 Feb 15 [PMID: 24277449] (IHC) IHC
Morifuji Yoshihiro, Onishi Hideya, Iwasaki Hironori et al. Reoxygenation from chronic hypoxia promotes metastatic processes in pancreatic cancer through the Hedgehog signaling. Cancer Sci. 2014 Feb 11 [PMID: 24397700]
Al-Ahmadie HA, Alden D, Qin LX et al. Carbonic anhydrase IX expression in clear cell renal cell carcinoma: an immunohistochemical study comparing 2 antibodies. Am J Surg Pathol 2008 Mar [PMID: 18300814] (IHC, Human) IHC Human
Skapa P, Hyrsl L, Zavada J et al. Carbonic anhydrase IX expression in clear cell renal cell carcinomas negatively correlates with the proportion of the granular cell component. J Clin Oncol 2008 Aug 1 [PMID: 18669473] (Human) Human
Bachmann IM, Ladstein RG, Straume O et al. Tumor necrosis is associated with increased alphavbeta3 integrin expression and poor prognosis in nodular cutaneous melanomas. BMC Cancer 2008 [PMID: 19061491] (IHC, Human) IHC Human
Dumais J. Plant morphogenesis: a role for mechanical signals. Curr Biol 2009 Mar 10 [PMID: 19278636] (IHC, Plant) IHC Plant
Nakao M, Ishii G, Nagai K et al. Prognostic significance of carbonic anhydrase IX expression by cancer-associated fibroblasts in lung adenocarcinoma. Cancer 2009 Jun 15 [PMID: 19365853] (WB, Human) WB Human
Di Napoli A, Signoretti S. Tissue biomarkers in renal cell carcinoma: issues and solutions. Cancer 2009 May 15 [PMID: 19402057] (Human) Human
Hong YS, Cho HJ, Kim SY et al. Carbonic anhydrase 9 is a predictive marker of survival benefit from lower dose of bevacizumab in patients with previously treated metastatic colorectal cancer. BMC Cancer 2009 [PMID: 19619339] (IHC, Human) IHC Human
Zhou GX, Ireland J, Rayman P et al. Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials. Urology 2010 Feb [PMID: 19963243] (ELISA, Human) ELISA Human
Park I, Larson PE, Zierhut ML et al. Hyperpolarized 13C magnetic resonance metabolic imaging: application to brain tumors. Neuro Oncol 2010 Feb [PMID: 20150380] (IHC) IHC
O'Toole D, Couvelard A, Rebours V et al. Molecular markers associated with response to chemotherapy in gastro-entero-pancreatic neuroendocrine tumors. Endocr Relat Cancer 2010 Dec [PMID: 20570957] (IHC, Human) IHC Human
Newton IP, Kenneth NS, Appleton PL et al. Adenomatous polyposis coli and hypoxia-inducible factor-1{alpha} have an antagonistic connection. Mol Biol Cell 2010 Nov 1 [PMID: 20844082] (ChIP) ChIP
Nelson MP, Christmann BS, Werner JL et al. IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina. J Immunol 2011 Feb 15 [PMID: 21220696] (ChIP) ChIP
Chaudary N, Pintilie M, Schwock J et al. Characterization of the Tumor-Microenvironment in Patient-Derived Cervix Xenografts (OCICx). Cancers (Basel) 2012 [PMID: 24213469] (IHC) IHC
Yoon C, Lee HJ, Park DJ et al. Hypoxia-activated chemotherapeutic TH-302 enhances the effects of VEGF-A inhibition and radiation on sarcomas. Br J Cancer 2015 May 26 [PMID: 26010414]
Yoon C, Lee HJ, Park DJ et al. Hypoxia-activated chemotherapeutic TH-302 enhances the effects of VEGF-A inhibition and radiation on sarcomas. Br. J. Cancer. 2015 May 26 [PMID: 26010414] (IHC-P, ICC/IF, Human) ICC/IF, IHC-P Human

Product General Protocols

View specific protocols for Carbonic Anhydrase IX/CA9 Antibody (NB100-417): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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FAQs for Carbonic Anhydrase IX/CA9 (9)

  1. How many units do we need to purchase in order to stain 150 formalin fixed, paraffin embedded kidney biopsies? It depends on the amount used per slide, so could you please confirm how much antibody do you use per slide in IHC-P? Also, is the ab compatible with ENVISION FLEX+, HIGH pH, FOR AS?
    • Unfortunately there is no number that I can provide to you as it is impossible to know for sure how many slides you will be able to stain with a vial. This will depend on your experimental conditions, your samples and how highly expressed the protein is, and what dilution you end up choosing. This antibody is recommended to be used at a 1:1000 dilution for IHC staining as this has worked well for our lab. You should order a single vial and test to determine what amount will be best for your assay rather than order too much or too little without first working with the antibody. As for your second question, that is not something that our lab has tested with so I am unsure if it is compatible or not. It has a fairly normal range pH in the buffer (7-8) so most likely the functions best around that range and not at a high pH.
  2. Could you please let me know the immunogen peptide location for this Ab?
    • The immunogen used to create this antibody was made to a cytoplasmic area of the protein.
  3. I'm going to use your CA-9 Antibody for paraffin-Immunohistochemistry. Do I have to do any special pretreatments?
    • Here is our protocol specific for this product. However, we recommend antigen retrieval to ensure the epitope is not masked, and we also recommend that you add a permeablizing agent to access the epitope in the nucleus.
  4. We dilute our antibodies in 5% milk TBS-Tween and usually freeze-thaw them multiple times. Is this possible with this antibody when diluted?
    • Freezing is not recommended for NB100-417 under any circumstances. Unfortunately, the antibody would no longer be covered by our guarantee if it was stored at a temperature that did not match our storage instructions. Of course, you are free to test your proposed conditions if you would like, but we would not be able to accept responsibility if the antibody was damaged by this.
  5. Is this antibody is carrier-free? If not, do you have a carrier-free version?
    • NB100-417 is carrier protein free, but does contain 0.1% sodium azide. The buffer formulation is: Tris-citrate/phosphate, pH 7-8 with 0.1% sodium azide. The following products are carrier protein and azide free (catalog numbers 23300002, H00000768-B01P and H00000768-D01P). If you would like to use NB100-417, we do offer antibody purification kits to remove unwanted buffer ingredients. See this link here for those products.
  6. I have a couple of questions regarding the Carbonic Anhydrase IX Antibody (NB100-417) listed on your website. We are wondering if this antibody is carrier-free, and if not, whether you have a carrier-free version?
    • Our NB100-417 is carrier protein free but does contain 0.1% sodium azide. The buffer formulation is: Tris-citrate/phosphate, pH 7-8 with 0.1% sodium azide. The following products are carrier protein and azide free (catalog numbers 23300002, H00000768-B01P and H00000768-D01P). If you would like to use NB100-417, we do offer antibody purification kits to remove unwanted buffer ingredients. See the link here for those products: AbSelect.
  7. I am interested in using the CA9 antibody (NB100-417) that you supply. We dilute our antibodies in 5% milk TBS-Tween and usually freeze-thaw them multiple times. Is this possible with this antibody when diluted?
    • I have checked with the lab and they have informed me that freezing is not recommended for NB100-417 under any circumstances. Unfortunately, the antibody would no longer be covered by our guarantee if it was stored at a temperature that did not match our storage instructions. Of course, you are free to test your proposed conditions if you would like, but we would not be able to accept responsibility if the antibody was damaged by this.
  8. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  9. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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