Carbonic Anhydrase IX Antibody 0.1 ml Hypoxia Marker

Carbonic Anhydrase IX NB100-417

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Ordering Information: NB100-417

Catalog NumberNB100-417
  • FormUnconjugated
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Carbonic Anhydrase IX Antibody Summary

 
Species Human, Mouse, Rat, Canine
Tested Applications WB, GS, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality Polyclonal
Host Rabbit
Gene CA9
Purity Immunogen affinity purified
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Carbonic Anhydrase IX Antibody Details

 
Marker Hypoxia Marker
Immunogen A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-500) [UniProt Q16790]
Localization Membrane.

Species Reactivity

 
Human, dog, rat and mouse (PMID 22842475).
Reviewed Species Read 3 rated 4.7  using NB100-417 in the following species:
 
Publications Read Publications using NB100-417 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 1:2000
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:1000
Application Notes

This Carbonic Anhydrase IX antibody is useful for Western blot, Immunofluorescence/Immunocytochemistry, and Immunohistochemistry. Gel Super Shift Assays was reported in scientific literature. In Western blot a band is observed ~53 kDa.

Molecular Weight

55 kDa

Positive Controls

3 Positive Controls

Other Available
Formats
Alexa Fluor (R) 405 Labeled NB100-417AF405
Alexa Fluor (R) 488 Labeled NB100-417AF488
Alexa Fluor (R) 647 Labeled NB100-417AF647
Alexa Fluor (R) 700 Labeled NB100-417AF700
Biotin Labeled NB100-417B
DyLight 405 Labeled NB100-417V
DyLight 488 Labeled NB100-417G
DyLight 550 Labeled NB100-417R
DyLight 650 Labeled NB100-417C
FITC Labeled NB100-417F
HRP Labeled NB100-417H
Learn about Custom Antibody Labeling
Control Peptide
Carbonic Anhydrase IX Peptide (NB100-417PEP)
Reviewed Applications
Read 2 reviews rated 5  using
NB100-417 in the following applications:
Publications
Read publications using
NB100-417 in the following applications:

Product PDFs

Reviews

Average Rating: 4.7 
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Publications

Carbonic Anhydrase IX Antibody (NB100-417) has been mentioned in at least 70 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

View all Carbonic Anhydrase IX Research Tools.

Packaging, Storage & Formulations

 
Storage Store at -20C. Avoid freeze-thaw cycles.
Buffer Tris-citrate/phosphate, pH 7-8
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration 1.0 mg/ml
Preservative 0.1% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol CA9
Entrez
Uniprot

Alternate Names for Carbonic Anhydrase IX

 
  • P54/58N
  • G250
  • CA-IX
  • EC 4.2.1.1
  • Renal cell carcinoma-associated antigen G250
  • RCC-associated protein G250
  • Membrane antigen MN
  • carbonic dehydratase
  • Carbonate dehydratase IX
  • CAIXcarbonic anhydrase 9
  • MNRCC-associated antigen G250
  • carbonic anhydrase IXpMW1

Related Products by Gene

Background

 
Carbonic anhydrase IX (CAIX) is a member of the Carbonic anhydrase family which are enzymes that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. They are abundant in all mammalian tissues and because of their functionality, they have become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). Carbonic Anhydrase IX (CA IX) is one of the most hypoxically inducible genes because of its stability and the location of the expressed protein within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX (CA9) results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.