Carbonic Anhydrase IX Antibody (NB100-417)
Carbonic Anhydrase IX Antibody
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Distributor Data...
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anti-RCC-associated antigen G250 antibody, anti-CA-IX antibody, anti-Carbonic anhydrase IX antibody, anti-Renal cell carcinoma associated antigen G250 antibody, anti-RCC associated protein G250 antibody, anti-pMW1 antibody, anti-P54/58N antibody, anti-P54 / 58N antibody, anti-Membrane antigen MN antibody, anti-G250 antibody, anti-Carbonic anhydrase 9 antibody, anti-Carbonate dehydratase IX antibody, anti-CA9 antibody, anti-CA IX antibody, anti-CAIX antibody, anti-MN antibody, anti-Carbonic dehydratase antibody |
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Carbonic Anhydrase IX Antibody Summary: | | Gene: | CA9 |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | Specificity: | This is specific for human CA IX (CA9). |
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| Publications: | Antibody has been mentioned in at least 18 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Carbonic Anhydrase IX Products Carbonic Anhydrase IX Antibody Details: | Immunogen: | A synthetic peptide derived from a C-terminal sequence of the human CA IX (CA9). |
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| Localization: | Found in cell membranes. |
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Reacts with human, dog and rat CA IX (carbonic Anhydrase IX). Other species have not been tested. |
| Uses: | NB100-417 is useful for western blot, immunofluorescence, and immunohistochemistry. See "Publications, Images, Reviews & Protocols" tab at the top, right of the datasheet, for the product specific IHC Protocol. |
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| Dilutions: | Immunofluorescence 1:1000, Immunohistochemistry-Frozen 1:1000, Immunohistochemistry-Paraffin, Western Blot 1:2000 |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 1.1 mg/ml |
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| Positive Controls: | 2 Positive Controls |
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| Conjugated Antibodies: | 5 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | Tris-citrate/phosphate, pH 7-8 |
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| Preservative: | 0.1% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC).
There are many genes that are inducible by hypoxia, via HIF-1 alpha. Carbonic Anhydrase IX (CA IX) is one of the most inducible genes because of its stability and the location of the expressed protein within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX (CA9) results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.
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Images (6) Reviews (1) Related Products (20) Exploring Carbonic Anhydrase IX Working with Carbonic Anhydrase IX
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with Carbonic Anhydrase IX
Carbonic Anhydrase IX Antibody Images (6) Immunofluorescence: Carbonic Anhydrase IX Antibody [NB100-417] - Immunofluorescence of HEK 293 cells using NB100-417. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock transfected cells. |  Immunofluorescence: Carbonic Anhydrase IX Antibody [NB100-417] - Immunofluorescence of human RCC tumor cryosections using NB100-417 (Panel A). Panel B shows staining with normal rabbit serum. |  Western Blot: Carbonic Anhydrase IX antibody [NB100-417] - Detection of carbonic anhydrase IX in transfected HEK cell lysate using NB 100-417. Rabbit IgG was used as a negative control. |  Immunohistochemistry: Carbonic Anhydrase IX antibody [NB100-417] - Renal carcinoma tissue stained with polyclonal carbonic anhydrase IX, NB 100-417. |  Western Blot: Carbonic Anhydrase IX Antibody [NB100-417] - Western blot analysis of CAIX on rat renal cortex using NB100-417. |  Western Blot: CAIX Antibody [NB100-417] - Western Blot on MDA-MB-231 cells |
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Reviewed by:
Anonymous 12/21/2011
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Application:
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IF
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Sample Tested:
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human cells
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Species:
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Human
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Sample Preparation:
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PFA fixed
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Sample Pretreatment:
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untreated
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Results:
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Really nice results using microscopy.
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Blocking Buffer:
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1% Donkey serum PBS
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Blocking Time:
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30 min
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Blocking Temperature:
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20 Celsius
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Dilution Ratio:
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1:250
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Incubation Time:
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1 hour
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Incubation Temperature:
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20C Celsius
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Incubation Dilution Buffer:
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PBS/1% Donkey serum
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Description:
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anti-rabbit alexa 488
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Dilution Ratio:
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1:500
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Visualization Method:
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fluorescence at 488
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Negative Control:
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secondary and primary only
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Publications (18)Carbonic Anhydrase IX Antibody Product Specific:- Garcia-Parra R, Wood D, Shah RB, et al. Investigation on tumor hypoxia in resectable primary prostate cancer as demonstrated by (18)F-FAZA PET/CT utilizing multimodality fusion techniques. Eur J Nucl Med Mol Imaging. 2011 Jul 16.[PMID 21833840]
- Al-Ahmadie HA, Alden D, Fine SW, et al. Role of immunohistochemistry in the evaluation of needle core biopsies in adult renal cortical tumors: an ex vivo study. Am. J. Surg. Pathol. 2011 Jul.[PMID 21677535]
more... - Chaumeil MM, Ozawa T, Park I, et al. Hyperpolarized 13C MR spectroscopic imaging can be used to monitor Everolimus treatment in vivo in an orthotopic rodent model of glioblastoma. NeuroImage.[PMID 21807103]
- Melvin A, Mudie S, Rocha S.The chromatin remodeler ISWI regulates the cellular response to hypoxia, role of FIH.Mol Biol Cell. 2011 Sep 7.[PMID: 21900490]
- Li Y, Wang H, Tu C et al. Role of hypoxia and EGF on expression, activity, localization and phosphorylation of carbonic anhydrase IX in MDA-MB-231 breast cancer cells. Biochim Biophys Acta. 2010 Oct 1. [PMID: 20920536]
- Lapinski JE, Chen L, Zhou M. Distinguishing clear cell renal cell carcinoma, retroperitoneal paraganglioma, and adrenal cortical lesions on limited biopsy material: utility of immunohistochemical markers.Appl Immunohistochem Mol Morphol. 2010 Oct;18(5):414-21.[PMID: 20861762]
- Narita T, Yin S, Gelin CF, et al. Identification of a Novel Small Molecule HIF-1{alpha} Translation Inhibitor. Clin Cancer Res 2009;15(19):6128-6136. [PMID: 19789328]
- Loinard C, Ginouves A, Vilar J, et al. Inhibition of Prolyl Hydroxylase Domain Proteins Promotes Therapeutic Revascularization. Circulation 2009;120(1):50-59. [PMID: 19546390] (Human, Mouse, WB)
- Chen J, Kobayashi M, Darmanin S, et al. Hypoxia-Mediated Up-Regulation of Pim-1 Contributes to Solid Tumor Formation. Am J Pathol 2009;175(1):400-411. [PMID: 19528349] (Mu, IHC-P)
- Lucio-Eterovic AK, Piao Y, de Groot JF. Mediators of Glioblastoma Resistance Invasion during Antivascular Endothelial Growth Factor Therapy. Clin Cancer Res 2009:1078-0432.CCR-1009-0575. [pubmed: 19567589]
- Banuelos CA, Banath JP, Kim J-Y, et al. {gamma}H2AX Expression in Tumors Exposed to Cisplatin Fractionated Irradiation. Clin Cancer Res 2009;15(10):3344-3353. [PMID: 19401347]
- Sakata K, Someya M, Nagakura H, et al. Brachytherapy for oral tongue cancer: an analysis of treatment results with various biological markers. Jpn J Clin Oncol. 2008 Jun;38(6):402-7. [PMID: 18573850] (Human, IHC-P)
- Westbury CB, Pearson A, Nerurkar A, et al. Hypoxia can be detected in irradiated normal human tissue: a study using the hypoxic marker pimonidazole hydrochloride. Br J Radiol. 2007 Nov;80(959):934-8. [PMID: 17908818] (Human, IHC-P)
- Kaidi A, Moorghen M, Williams AC, et al. Is the downregulation of EphB2 receptor expression during colorectal tumorigenesis due to hypoxia? Gut. 2007 Nov;56(11):1637-8. [PMID: 17938437]
- Sathornsumetee S, Cao Y, Marcello JE, et al. Tumor angiogenic and hypoxic profiles predict radiographic response and survival in malignant astrocytoma patients treated with bevacizumab and irinotecan. J Clin Oncol. 2008 Jan 10;26(2):271-8. [PMID: 1818266] (Human, IHC-P)
- Van den Eynden GG, Van der Auwera I, Van Laere SJ, et al. Angiogenesis and hypoxia in lymph node metastases is predicted by the angiogenesis and hypoxia in the primary tumour in patients with breast cancer. Br J Cancer. 2005 Nov 14;93(10):1128-36. [PMID: 16251878] (IHC-P, Human)
- Atkins M, Regan M, McDermott D, et al. Carbonic anhydrase IX expression predicts outcome of interleukin 2 therapy for renal cancer. Clin Cancer Res. 2005 May 15;11(10):3714-21. [PMID: 15897568]
- Yoo H, Baia GS, Smith JS, et al. Expression of the hypoxia marker carbonic anhydrase 9 is associated with anaplastic phenotypes in meningiomas. Clin Cancer Res. 2007 Jan 1;13(1):68-75. [PMID: 17200340] (IHC-P, Human)
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Jump to: Conjugated Antibodies, Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Carbonic Anhydrase IX Antibody [Biotin] | NB100-417B | Ca, Hu | IF, IHC-P, WB | (4) | (6) | | | | Carbonic Anhydrase IX Antibody [HRP] | NB100-417H | Ca, Hu | IF, IHC-P, WB | (4) | (6) | | | | Carbonic Anhydrase IX Antibody [DyLight 488] | NB100-417G | Ca, Hu | IF, IHC-P, WB | | (8) | | | | Carbonic Anhydrase IX Antibody [DyLight 550] | NB100-417R | Ca, Hu | IF, IHC-P, WB | | (8) | | | | Carbonic Anhydrase IX Antibody [DyLight 650] | NB100-417C | Ca, Hu | IF, IHC-P, WB | | (8) | | |
Positive ControlsAntibody Pairs (1)Lysates (2)Peptides and ProteinsPrimary Antibodies (12)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Carbonic Anhydrase IX Antibody (2D3) | NBP1-51691 | Hu | ELISA, IF, IHC-P, WB | (3) | | | | | Carbonic Anhydrase IX Antibody (1G7) | NBP1-47688 | Hu | FACS, IHC-P, WB | (3) | | | | | Carbonic Anhydrase IX Antibody (pT443) | NBP1-33781 | Hu | ELISA, IP, WB | | | | | | Carbonic Anhydrase IX Antibody | 23300002 | Hu | ELISA, IHC-P, WB | | | | | | Carbonic Anhydrase IX Antibody | H00000768-B01P | Hu | ELISA, WB | (1) | (1) | | | | ... and 7 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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