CYP27A1 Antibody (NB100-92422)

CYP27A1 Antibody

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CYP27A1 Antibody Summary:
Species:Hu
Applications:ELISA, IHC-P, WB
Clonality:Polyclonal
Gene:CYP27A1
Purity:Immunogen affinity purified
Host:Rabbit
 
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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CYP27A1 Antibody Details:
Isotype:IgG
Immunogen:Synthetic peptide corresponing to the residues surrounding Glutamate 131 of human CYP27A1
Species Reactivity:

This antibody is reactive against Human.

Applications:
Uses:Western blot analysis of CYP27A1 (E131) antibody in extracts from HeLa cells.
Dilutions:ELISA 1:10000-1:20000, Immunohistochemistry-Paraffin, Western Blot 1:500-1:1000
Unit Size:0.1 mg
Concentration:1.0 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:Phosphate buffered saline, pH 7.2.
Preservative:15mM Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: CYP27A1
Entrez1593 (Human)
 
Background:

The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans. CyPA is involved in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

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Working with CYP27A1

CYP27A1 Antibody Images (2)
Western Blot: CYP27A1 Antibody [NB100-92422] - Western blot (WB) analyzes of CYP27A1 (E131) antibody in extracts from HeLa cellsImmunohistochemistry-Paraffin: CYP27A1 Antibody [NB100-92422] - Paraffin-embedded human lung carcinoma tissue.
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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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