COS-7 Hypoxic Induced Control Lysate (NB800-PC26)

COS-7 Hypoxic Induced Control Lysate

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COS-7 Hypoxic Induced Control Lysate Summary:

Applications:	WB

Purity:	Protein

Specificity:	NB800-PC26 contains hypoxia induced and uninduced COS-7 nuclear extract lysate
Publications:	Antibody has been mentioned in at least 2 Publications.

Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.

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Lysate Details:

Type:	Cell	Life Stage:	Adult
Tissue:	Digestive/Waste	Tissue Condition:	Hypoxia
Sub-cellular Fraction:	Nuclear

Applications:

Uses:	Extracts have been quality control tested by Western blot. Before use mix 1:1 with 2X Sample Buffer and heat to 90C for 5 minutes before running on gel.
Dilutions:	Western Blot: load 50ug per lane
Unit Size:	4 Vials
Concentration:	2.5 mg/ml

Notes:

NB800-PC26 packaging includes 0.2 mg untreated and 0.2 mg treated of COS-7 nuclear extract. This kit is shipped on dry ice.

Packaging:

Storage:	Store at -80 °C. Avoid freeze-thaw cycles.
Buffer:	20mM Hepes buffer, pH 7.5, with 400mM NaCl, 20% glycerol, 0.1mM EDTA, 10mM NaF, 10µM NaMoO4, 1mM NaV
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Background:

When studying proteins that require hypoxic induction prior to expression this kit is a positive control for these experiments. These positive and negative control samples in NB800-PC26 are induced with cobalt chloride.

Reviews (1)

Mingfang Tao on 10/24/2011

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Reviewed by:
Mingfang Tao 10/24/2011

Application:	Western Blot
Sample Tested:	Rat bladder tissue protein
Sample Loaded:	50ug
Species:	Rat
Sample Pretreated:	RIPA buffer

Blocking Buffer:	5% nonfat Milk in TBST
Blocking Time:	1 hour
Blocking Temperature:	RT

Dilution Ratio:	1:300
Incubation Time:	overnight
Incubation Temperature:	4C
Incubation Dilution Buffer:	TBST

Description:	goat anti-mouse
Manufacturer Catalog #:	Millipore AP124P
Dilution Ratio:	1:2000

Method:	ECL
Exposure Time:	30 min
Positive Control:	HIF-1 alpha
Did you observe bands?:	Yes
Specific Bands:	120 kDa kDa
Non-specific Bands:	70, 55, 25 kDa kDa

Publications (2)

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