CLOCK Antibody 0.1 ml

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Ordering Information: NBP1-30326

  • Catalog Number
    NBP1-30326
  • Displayed Format
    Unconjugated
  • Other Formats
  • Size(s)
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    CLOCK Antibody Summary

    Species Human, Mouse
    Predicted Species
    Rat (94%). Backed by our 100% Guarantee
    Tested Applications ICC/IF (-), WB, IHC
    Clonality
    Polyclonal
    Host
    Rabbit
    Gene
    CLOCK
    Purity
    Immunogen affinity purified
    Guarantee Plus
    All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
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    CLOCK Antibody Details

    Immunogen
    Synthetic peptide made to a C-terminal portion of mouse CLOCK (within residues 805-855). [Swiss-Prot# O08785]
    Localization
    Cytoplasmic and Nuclear. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus.

    Species Reactivity

    Human and mouse. Immunogen has 94% identity with rat. Other species have not been tested.

    Applications/Dilutions

    Dilutions
    • ICC/IF (Negative)
    • Western Blot 1:1000
    • Immunohistochemistry 1:100
    Application Notes
    This CLOCK antibody is useful for Western blot analysis where a band can be seen at 95 kDa. This antibody is not recommended for Immunocytochemistry/Immunofluorescence.

    The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    Molecular Weight
    95 kDa
    Positive Controls
    Other Available
    Formats
    DyLight 405 Labeled NBP1-30326V
    Control Peptide
    CLOCK Peptide (NBP1-30326PEP)

    Contact Information

    Product PDFs

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    Packaging, Storage & Formulations

    Storage
    Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
    Buffer
    PBS, 30% glycerol
    Unit Size
    0.1 ml
    Concentration
    1 mg/ml
    Preservative
    0.1% Sodium Azide
    Limitations
    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

    Bioinformatics

    Gene Symbol CLOCK
    Entrez
    Uniprot

    Alternate Names for CLOCK Antibody

    • BHLHE8
    • circadian locomoter output cycles protein kaput
    • Class E basic helix-loop-helix protein 8
    • clock (mouse) homolog
    • clock homolog (mouse)
    • EC 2.3.1.48
    • hCLOCK
    • KAT13D
    • KIAA0334bHLHe8circadian locomoter output cycles kaput protein
    Show More

    Related Products by Gene

    Background

    Different physiological as well as behavioral processes such as sleep-wake cycles, thermoregulation, hormonal secretions, blood pressure and metabolism exhibits 24-hour rhythmicity which is generated through a molecular machinery involving transcriptional-translational feedback loop in the suprachiasmatic nucleus (SCN) of hypothalamus and many peripheral tissues. Mammalian circadian system contains a set of core CLOCK genes that encode proteins such as CLOCK (circadian locomotor output kaput), BMAL1 (brain and muscle ARNLT1), PER (period), CRY (cryptochrome), REV-ERB alpha, and ROR alpha. CLOCK was the first gene identified in its family and CLOCK protein binds DNA for activating transcription after dimerization with BMAL1 by driving the rhythmic transcription of other CLOCK and circadian clock-controlled genes through an E-box (5'-CACGTG-3'). It activates transcription of PER1/PER2 which is inhibited in a feedback loop by PER and CRY proteins. With its intrinsic histone acetyltransferase activity, CLOCK acetylates histones H3, histone H4, and ARNTL (non-histone substrate), and has also been shown to plays a role in DNA damage response/DDR signaling during the S phase of cell cycle. Because this endogenous timekeeper interacts with countless biological systems, CLOCK dysfunctions may lead to the pathogenesis of several diseases including sleep disorders, metabolic syndrome, cardiovascular and inflammatory disease, and cancer.

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    Product General Protocols

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    Video Protocols

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