CLOCK Antibody 0.1 ml

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Western Blot: CLOCK Antibody [NBP1-30326] - Analysis of CLOCK in Sol8 (mouse embryo fibroblast) cell extracts using NBP1-30326.
Immunohistochemistry: CLOCK Antibody [NBP1-30326] - CLOCK antibody was tested in mouse brain using DAB with hematoxylin counterstain.
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Ordering Information: NBP1-30326

  • Catalog Number
    NBP1-30326
  • Displayed Format
    Unconjugated
  • Other Formats
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CLOCK Antibody Summary

Species Human, Mouse
Predicted Species
Rat (94%). Backed by our 100% Guarantee
Tested Applications ICC/IF (-), WB, IHC
Clonality
Polyclonal
Host
Rabbit
Gene
CLOCK
Purity
Immunogen affinity purified
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CLOCK Antibody Details

Immunogen
Synthetic peptide made to a C-terminal portion of mouse CLOCK (within residues 805-855). [Swiss-Prot# O08785]
Localization
Cytoplasmic and Nuclear. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus.

Species Reactivity

Human and mouse. Immunogen has 94% identity with rat. Other species have not been tested.

Applications/Dilutions

Dilutions
  • ICC/IF (Negative)
  • Western Blot 1:1000
  • Immunohistochemistry 1:100
Application Notes
This CLOCK antibody is useful for Western blot analysis where a band can be seen at 95 kDa. This antibody is not recommended for Immunocytochemistry/Immunofluorescence.
Molecular Weight
95 kDa
Positive Controls
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-30326AF405
Alexa Fluor (R) 488 Labeled NBP1-30326AF488
Alexa Fluor (R) 647 Labeled NBP1-30326AF647
Alexa Fluor (R) 700 Labeled NBP1-30326AF700
Biotin Labeled NBP1-30326B
DyLight 405 Labeled NBP1-30326V
DyLight 488 Labeled NBP1-30326G
DyLight 550 Labeled NBP1-30326R
DyLight 650 Labeled NBP1-30326C
FITC Labeled NBP1-30326F
HRP Labeled NBP1-30326H
Control Peptide
CLOCK Peptide (NBP1-30326PEP)

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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% glycerol
Unit Size
0.1 ml
Concentration
1 mg/ml
Preservative
0.1% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol CLOCK
Entrez
Uniprot

Alternate Names for CLOCK Antibody

  • BHLHE8
  • circadian locomoter output cycles protein kaput
  • Class E basic helix-loop-helix protein 8
  • clock (mouse) homolog
  • clock homolog (mouse)
  • EC 2.3.1.48
  • hCLOCK
  • KAT13D
  • KIAA0334bHLHe8circadian locomoter output cycles kaput protein
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Related Products by Gene

Background

Different physiological as well as behavioral processes such as sleep-wake cycles, thermoregulation, hormonal secretions, blood pressure and metabolism exhibits 24-hour rhythmicity which is generated through a molecular machinery involving transcriptional-translational feedback loop in the suprachiasmatic nucleus (SCN) of hypothalamus and many peripheral tissues. Mammalian circadian system contains a set of core CLOCK genes that encode proteins such as CLOCK (circadian locomotor output kaput), BMAL1 (brain and muscle ARNLT1), PER (period), CRY (cryptochrome), REV-ERB alpha, and ROR alpha. CLOCK was the first gene identified in its family and CLOCK protein binds DNA for activating transcription after dimerization with BMAL1 by driving the rhythmic transcription of other CLOCK and circadian clock-controlled genes through an E-box (5'-CACGTG-3'). It activates transcription of PER1/PER2 which is inhibited in a feedback loop by PER and CRY proteins. With its intrinsic histone acetyltransferase activity, CLOCK acetylates histones H3, histone H4, and ARNTL (non-histone substrate), and has also been shown to plays a role in DNA damage response/DDR signaling during the S phase of cell cycle. Because this endogenous timekeeper interacts with countless biological systems, CLOCK dysfunctions may lead to the pathogenesis of several diseases including sleep disorders, metabolic syndrome, cardiovascular and inflammatory disease, and cancer.

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There are no publications for CLOCK Antibody (NBP1-30326).
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Points can be redeemed for discounts and gift cards:
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Items ships for Free, please disregard any shipping charges.
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Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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FAQs for CLOCK (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)