CLOCK Antibody 0.1 ml

CLOCK NBP1-30326

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Ordering Information: NBP1-30326

Catalog NumberNBP1-30326
  • FormUnconjugated
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CLOCK Antibody Summary

 
Species Human, Mouse
Predicted Species Rat (94%). Backed by our 100% guarantee.
Tested Applications WB, IHC, ICC/IF (-)
Clonality Polyclonal
Host Rabbit
Gene CLOCK
Purity Immunogen affinity purified
Guarantee Plus All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
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CLOCK Antibody Details

 
Immunogen Synthetic peptide made to a C-terminal portion of mouse CLOCK (within residues 805-855). [Swiss-Prot# O08785]
Localization Cytoplasmic and Nuclear. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus.

Species Reactivity

 
Human and mouse. Immunogen has 94% identity with rat. Other species have not been tested.

Applications/Dilutions

 
Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:100
  • ICC/IF (Negative)
Application Notes

This CLOCK antibody is useful for Western blot analysis where a band can be seen at 95 kDa. This antibody is not recommended for Immunocytochemistry/Immunofluorescence.

Molecular Weight

95 kDa

Positive Controls

1 Positive Control

Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-30326AF405
Alexa Fluor (R) 488 Labeled NBP1-30326AF488
Alexa Fluor (R) 647 Labeled NBP1-30326AF647
Alexa Fluor (R) 700 Labeled NBP1-30326AF700
Biotin Labeled NBP1-30326B
DyLight 405 Labeled NBP1-30326V
DyLight 488 Labeled NBP1-30326G
DyLight 550 Labeled NBP1-30326R
DyLight 650 Labeled NBP1-30326C
FITC Labeled NBP1-30326F
HRP Labeled NBP1-30326H
Learn about Custom Antibody Labeling
Control Peptide
CLOCK Peptide (NBP1-30326PEP)

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Controls & Support Products

Research Tools

View all CLOCK Research Tools.

Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer PBS, 30% glycerol
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration 1 mg/ml
Preservative 0.1% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol CLOCK
Entrez
Uniprot

Alternate Names for CLOCK

 
  • clock (mouse) homolog
  • hCLOCK
  • EC 2.3.1.48
  • BHLHE8
  • Class E basic helix-loop-helix protein 8
  • circadian locomoter output cycles protein kaput
  • KAT13D
  • KIAA0334bHLHe8circadian locomoter output cycles kaput protein
  • clock homolog (mouse)

Related Products by Gene

Background

 
Different physiological as well as behavioral processes such as sleep-wake cycles, thermoregulation, hormonal secretions, blood pressure and metabolism exhibits 24-hour rhythmicity which is generated through a molecular machinery involving transcriptional-translational feedback loop in the suprachiasmatic nucleus (SCN) of hypothalamus and many peripheral tissues. Mammalian circadian system contains a set of core CLOCK genes that encode proteins such as CLOCK (circadian locomotor output kaput), BMAL1 (brain and muscle ARNLT1), PER (period), CRY (cryptochrome), REV-ERB alpha, and ROR alpha. CLOCK was the first gene identified in its family and CLOCK protein binds DNA for activating transcription after dimerization with BMAL1 by driving the rhythmic transcription of other CLOCK and circadian clock-controlled genes through an E-box (5'-CACGTG-3'). It activates transcription of PER1/PER2 which is inhibited in a feedback loop by PER and CRY proteins. With its intrinsic histone acetyltransferase activity, CLOCK acetylates histones H3, histone H4, and ARNTL (non-histone substrate), and has also been shown to plays a role in DNA damage response/DDR signaling during the S phase of cell cycle. Because this endogenous timekeeper interacts with countless biological systems, CLOCK dysfunctions may lead to the pathogenesis of several diseases including sleep disorders, metabolic syndrome, cardiovascular and inflammatory disease, and cancer.