CDC42 Protein Summary:

Species:	Hu
Protein Type:	Full length Protein
Applications:	ELISA, PAGE

Gene:	CDC42
Purity:	Protein

Specificity:	Human, T7-tagged, Recombinant CDC42, 1- 188 aa

Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.

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Lysates	Peptides and Proteins
Primary Antibodies	RNAi

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Species Reactivity:

Human Protein.

Applications:

Uses:	This product is useful for ELISA and SDS-Page. The purity of this protein is > 95% by SDS - PAGE. This recombinant protein was expressed in E.Coli bacteria. The endotoxin levels < 1.0 EU per 1 microgram of protein (determined by LAL method ).
Dilutions:	ELISA, SDS-Page
TMW:	22.4 kDa
Unit Size:	0.1 mg
Concentration:	1.0 mg/ml

Packaging:

Storage:	Store at -80 °C. Avoid freeze-thaw cycles.
Buffer:	20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: CDC42

Entrez	998 (Human)
Swiss Prot	NP_001782 (Human)

Background:

Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs. Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Working with CDC42

CDC42 Protein Images (1)

SDS-Page: CDC42 Protein [NBP1-30243] - CDC42, 22.4 kDa (203aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

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Jump to: Lysates, Peptides and Proteins, Primary Antibodies, RNAi

Related Products

Lysates (3)

Name	Catalog#	Applications	Images
CDC42 Lysate	NBL1-09004	WB	(1)
CDC42 Lysate	NBL1-09005	WB	(1)
CDC42 Lysate	H00000998-T02	WB

Peptides and Proteins

Name	Catalog#	Reactivity	Applications	Images
CDC42 Partial Recombinant Protein	H00000998-Q01	Hu	ELISA, WB	(1)
CDC42 Recombinant Protein	H00000998-P02	Hu	ELISA, WB	(1)
CDC42 Recombinant Protein	H00000998-P01	Hu	ELISA, WB	(1)
CDC42 Peptide	NBP1-76330PEP	Hu	B/N

Primary Antibodies (5)

Name	Catalog#	Reactivity	Applications	Images	Publications
CDC42 Antibody	NBP1-45477	Bv, Hu, Mu, Rt	IHC-P, WB	(1)
CDC42 Antibody	NBP1-76330	Hu, Mu, Rt	ELISA, WB	(1)
CDC42 Antibody	NB120-14241	Hu, Mu, Rt	WB	(1)	(1)
CDC42 [p Ser71] Antibody (EP221Y)	NBP1-44435	Hu	ICC, WB
CDC42 Antibody	H00000998-D01P	Hu	ELISA, WB	(1)

RNAi (2)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
CDC42 RNAi	H00000998-R01	Hu		(1)	(4)
CDC42 RNAi	H00000998-R02	Hu		(1)	(4)

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CDC42 in Context

Research Areas related to CDC42 (3):

Novus Explorer for CDC42 gene

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CDC42 Protein (NBP1-30243)

CDC42 Protein