CDC42 Lysate (NBL1-09004)

CDC42 Lysate

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CDC42 Lysate Summary:
Applications:WB
Gene:CDC42
Purity:Protein
Specificity:Homo sapiens cell division cycle 42 (GTP binding protein, 25kDa) (CDC42), transcript variant 1, mRNA.
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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CDC42 Lysate Details:
Immunogen:The lysate was created in HEK293T cells, using plasmid ID RC214076 and based on accession number NM_001791. The protein contains a C-terminal DDK tag.
Lysate Details:
Type: OverexpressionLife Stage: Adult
Protein State: Native Tissue Condition: Normal
Sub-cellular Fraction: Whole
Applications:
Uses:This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Dilutions:Western Blot
TMW:21.1 kDa
Unit Size:0.1 mg
Concentration:1.0 mg/ml
Notes:
This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes. Please note that “1 mg/ml” refers to the total protein concentration and that the specific protein concentration is not determined at this time.
Packaging:
Storage:Store at -80 °C. Avoid freeze-thaw cycles.
Buffer:RIPA buffer
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: CDC42
Entrez998 (Human)
Swiss ProtNM_001791 (Human)
 
Background:

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (1) encodes isoform 1 which has the same aa length but different C-terminus from isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

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Working with CDC42

CDC42 Lysate Images (1)
Western Blot: CDC42 Lysate [NBL1-09004] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for CDC42
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CDC42 in Context


Research Areas related to CDC42 (3):
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