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CD44 Antibody (NB500-481)

CD44 Antibody (MEM-263)

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CD44 Antibody (MEM-263) Summary:
Species:Hu
Applications:FACS, IHC-P, IP, WB
Clonality:Monoclonal
Gene:CD44
Purity:Affinity purified
Host:Mouse
Specificity:Reacts with extracellular (N-terminal) domain of standard CD44.
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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CD44 Antibody (MEM-263) Details:
Clone:MEM-263
Isotype:IgG1
Immunogen:COS-7 cells.
Marker:Cell membrane Marker
Species Reactivity:

Cross-reacts with Human. Not yet tested in other species.

Applications:
Uses:Flow Cytometry Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Dilutions:Flow Cytometry, ImmunoPrecipitation 1:50, Western Blot 1:1000, Immunohistochemistry-Paraffin 1:200
Unit Size:0.1 mg
Concentration:1 mg/ml
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:PBS [pH 7.4]
Preservative:15mM Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: CD44
Entrez960 (Human)
 
Background:

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Images (1)
  • Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.

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CD44 Antibody (MEM-263) Images (1)
Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL.
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