The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
This antibody is useful in Flow Cytometry, immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry. Purity is > 95% (by SDS-PAGE)
Dilutions:
Flow Cytometry,
ImmunoPrecipitation 1:50,
Western Blot 1:1000,
Immunohistochemistry-Paraffin 1:200,
Immunohistochemistry-Frozen 1:200,
Immunocytochemistry
Unit Size:
0.1 mg
Concentration:
1 mg/ml
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
PBS [pH 7.4]
Preservative:
15mM Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
CD44 is a transmembrane glycoprotein expressed on the surface of most cells,
which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell
adhesion, proliferation and migration, it is thus important for lymphocyte activation,
recirculation and homing. Although CD44 functions are essential for physiological
activities of normal cells, elevated CD44 expression correlates with poor prognosis
in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and
directional motility of cancer cells.