CD44 Antibody (Bu52) Summary:

Species:	Hu
Applications:	FACS, IHC-Fr, IHC-P, IP
Clonality:	Monoclonal

Gene:	CD44
Purity:	Protein G purified
Host:	Mouse

Specificity:

NB100-64349 recognizes the human CD44 cell surface antigen, a 100kD glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and of the breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. The Bu52 antibody recognizes an epitope that is common to all CD44 isoforms. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

Publications:

Antibody has at least 1 Background Reference.

Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.

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CD44 Antibody (Bu52) Details:

Clone:	Bu52
Isotype:	IgG1
Immunogen:	Made to Human CD44
Marker:	Cell membrane Marker

Species Reactivity:

Cross reacts with Human.

Applications:

Uses:	Flow Cytometry, Immunohistochemistry-Frozen and Paraffin, Immunoprecipitation
Dilutions:	Flow Cytometry 1:25 - 1:100, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 1:100 - 1:200, Immunoprecipitation
Unit Size:	0.2 mg
Concentration:	1.0 mg/ml

Packaging:

Storage:	Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:	Phosphate buffered saline pH7.4
Preservative:	0.09% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: CD44

Entrez	960 (Human)
Swiss Prot	P16070 (Human)

Background:

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

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Working with CD44

CD44 Antibody (Bu52) Images (1)

Immunohistochemistry-Paraffin: CD44 Antibody (Bu52) [NB100-64349] - human tonsil with Mouse anti Human CD44

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Publications (1)

CD44 Antibody (Bu52) Background References:

Takahashi, K. et al. (1996) Keratan sulfate modification of CD44 modulates adhesion to hyaluronate. J Biol Chem.271: 9490

Protocols

All General Protocols

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Jump to: Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

Related Products

Lysates (3)

Name	Catalog#	Reactivity	Applications	Images
CD44 Lysate	H00000960-T01	Hu	WB	(2)
CD44 Lysate	NBL1-08952		WB	(1)
CD44 Lysate	H00000960-T02		WB

Peptides and Proteins

Name	Catalog#	Reactivity	Applications	Images
CD44 Recombinant Protein	H00000960-P02	Hu	ELISA, WB	(1)
CD44 Recombinant Protein	H00000960-P01		ELISA, WB	(1)
CD44 Protein	H00000960-G01	Hu	Func
CD44 Protein	NBP1-94196	Hu

Primary Antibodies (79)

Name	Catalog#	Reactivity	Applications	Images	Publications
CD44 Antibody (8E2F3)	NBP1-47386	Hu, Mu	ELISA, IF, IHC-Fr, IHC-P, WB	(7)
CD44 Antibody	NBP1-31488	Hu	ICC, IF, IHC-P, WB	(6)
CD44 Antibody (IM7)	NBP1-41266	Ca, Eq, Fe, Hu, Mu	FACS, ICC, IHC-Fr, IHC-P, IP, WB
CD44 Antibody (EPR1013Y)	NB110-55700	Hu, Mu(-), Rt(-)	FACS, ICC, IHC-P, WB	(2)	(3)
CD44 Antibody	NBP1-84578	Hu	IF, IHC-P, WB	(3)
... and 74 more. See All »

RNAi (5)

Name	Catalog#	Reactivity	Images	Publications
CD44 RNAi	H00000960-R10	Hu	(1)	(4)
CD44 RNAi	H00000960-R07	Hu	(1)	(4)
CD44 RNAi	H00000960-R08	Hu	(1)	(4)
CD44 RNAi	H00000960-R06	Hu	(1)	(4)
CD44 RNAi	H00000960-R09	Hu	(1)	(4)

Secondary Antibodies

Name	Catalog#	Reactivity	Applications	Publications
Mouse IgG Antibody [Biotin]	NB720-B	Mu	ELISA, IHC-P, WB
Mouse IgG Antibody [HRP]	NB720-H	Mu	ELISA, ICC, IHC-P, WB
Mouse IgG Antibody [Alkaline Phosphatase]	NB720-AP	Mu	ELISA, ICC, IHC-P, WB
Mouse IgG Antibody [FITC]	NB720-F	Mu	ICC, IHC-P	(2)
Mouse IgG Antibody	NB120-7056	Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-)	ELISA, IHC-P, IP, WB
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CD44 in Context

Research Areas related to CD44 (9):

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CD44 Antibody (NB100-64349)

CD44 Antibody (Bu52)