CD44 Antibody (8E2F3) (NBP1-47386)

CD44 Antibody (8E2F3) (NBP1-47386) (0.1 ml)

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	Catalog Number	NBP1-47386
	Size(s)	0.1 ml	Price:
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anti-Phagocytic glycoprotein I antibody, anti-Hermes antigen antibody, anti-PGP-I antibody, anti-GP90 lymphocyte homing/adhesion receptor antibody, anti-PGP-1 antibody, anti-CD44 antigen antibody, anti-Epican antibody, anti-LHR antibody, anti-CDw44 antibody, anti-HUTCH-I antibody, anti-homing function and Indian blood group system antibody, anti-ECMR-III antibody, anti-hematopoietic cell E- and L-selectin ligand antibody, anti-Phagocytic glycoprotein 1 antibody, anti-epican antibody, anti-Hyaluronate receptor antibody, anti-chondroitin sulfate proteoglycan 8 antibody, anti-Heparan sulfate proteoglycan antibody, anti-cell surface glycoprotein CD44 antibody, anti-Extracellular matrix receptor III antibody, anti-Pgp1 antibody, anti-MC56 antibody, anti-IN antibody, anti-HCELL antibody, anti-CSPG8 antibody, anti-MIC4MGC10468 antibody, anti-MDU3CDW44 antibody, anti-MDU2CD44 antigen (homing function and Indian blood group system) antibody, anti-CD44R antibody, anti-CD44 molecule (Indian blood group) antibody

CD44 Antibody (8E2F3) Summary:

Species:	Hu, Mu	Gene:	CD44
Applications:	WB, ELISA, FACS, ICC, IF, IHC-P	Purity:	Ascites
Clonality:	Monoclonal	Host:	Mouse
Publications:

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CD44 Antibody (8E2F3) Details:

Clone:	8E2F3
Isotype:	IgG1
Immunogen:	Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli.
Marker:	Cell membrane Marker

Species Reactivity:

Human, Mouse

Applications:

Uses:	This antibody is useful in Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, ELISA.
Dilutions:	ELISA 1:10000, Flow Cytometry 1:200-1:400, Immunocytochemistry 1:200-1:1000, Immunofluorescence 1:200 - 1:1000, Immunohistochemistry-Paraffin 1:200 - 1:1000, Western Blot 1: 500 - 1:2000
MW:	82 kDa
Unit Size:	0.1 ml
Concentration:	This product is unpurified. Concentration is not relevant.

Packaging:

Storage:	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Buffer:	Ascites
Preservative:	0.03% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: CD44

Entrez:	960 (Human)
Uniprot:	P16070 (Human)

Related Genes:

Background:

The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.

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CD44 Antibody (8E2F3) Images (7)

	Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Western blot analysis using anti-CD44 mAb against Hela (1) and HUVE-12(2) cell lysate.

	Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Western blot analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).

	Immunohistochemistry: CD44 Antibody (8E2F3) [NBP1-47386] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using anti-CD44 mAb.

	Flow cytometry / FACS analysis: CD44 Antibody (8E2F3) [NBP1-47386] - Flow cytometric analysis of Hela cells using anti-CD44 mAb (right) and negative control (left).

	Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.

	Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal immunofluorescence analysis of PANC-1 cells using anti-CD44 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

	Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.