CD44 Antibody (156-3C11) Summary:

Species:	Bb, Hu, Mk
Applications:	IHC-P, IP, WB
Clonality:	Monoclonal

Gene:	CD44
Purity:	Protein A purified
Host:	Mouse

Specificity:

This antibody reacts with red cells pretreated with 2-aminoethylisothiouronium bromide (AET). Digestion of intact erythrocytes by trypsin treatment leaves a 50-54kDa protein fragment attached to the membrane, while chymotrypsin leaves a somewhat similarly sized 51-56kDa. This antibody enhances IL-2 production by CD2-transfected murine hybridoma stimulated by JY cells while it has no significant effect on IL-2 production by Jurkat cells stimulated with CD3, PMA, or CD2.

Publications:

Antibody has at least 19 Background References.

Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.

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RNAi

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CD44 Antibody (156-3C11) Details:

Clone:	156-3C11
Isotype:	IgG2a
Immunogen:	Stimulated human leukocytes.
Localization:	Cell Membrane
Marker:	Cell membrane Marker
Epitope:	Resistant to digestion by trypsin and chymotrypsin.1

Species Reactivity:

Cross-reacts with Human, Baboon and Green Monkey. Not yet tested in other species.

Applications:

Uses:	IP: (Native verified) (Use Protein A) IHC: (Formalin/paraffin). Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0.
Dilutions:	immunoprecipitation 2 ug/ml, Western Blot 1-2 ug/ml for 2 hrs at RT, Immunohistochemistry-Paraffin 1-2 ug/ml 30 min at RT
Unit Size:	0.25 ml
Concentration:	0.2 mg/ml
Positive Controls:	1 Positive Controls

Packaging:

Storage:	Store at 4 °C. Do not freeze.
Buffer:	10mM PBS, pH 7.4, with 2.0% BSA
Preservative:	0.09% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: CD44

Entrez	960 (Human)

Background:

CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule (HCAM), Phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing, and in tumor metastasis.

Images (1)

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Working with CD44

CD44 Antibody (156-3C11) Images (1)

Formalin-fixed, paraffin-embedded human esophageal carcinoma stained with CD44 using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of tumor cells.

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Publications (19)

CD44 Antibody (156-3C11) Background References:

Kuppner MC; Hamou MF; de Tribolet N. Activation adhesion molecule expression on lymphoid infiltrates in human glioblastomas. Journal of Neuroimmunology, 1990, 29(1-3):229-38.
Wang C; Tammi M; Tammi R. Distribution of hyaluronan its CD44 receptor in the epithelia of human skin appendages. Histochemistry, 1992, 98(2):105-12.

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Vogel H; Butcher EC; Picker LJ. H-CAM expression in the human nervous system: evidence for a role in diverse glial interactions. Journal of Neurocytology, 1992, 21(5):363-73.
Skov BG; Hirsch FR; Bobrow L. Monoclonal antibodies in the detection of bone marrow metastases in small cell lung cancer. British Journal of Cancer, 1992, 65(4):593-6.
Foets BJ; van den Oord JJ; Volpes R; Missotten L. In situ immunohistochemical analysis of cell adhesion molecules on human corneal endothelial cells. British Journal of Ophthalmology, 1992,76(4):205-9.
Wielenga VJ; Heider KH; Offerhaus GJ; Adolf GR; van den Berg FM; Ponta H; Herrlich P; Pals ST. Expression of CD44 variant proteins in human colorectal cancer is related to tumor progression. Cancer Research, 1993, 53(20):4754-6.
Underhill CB; Nguyen HA; Shizari M; Culty M. CD44 positive macrophages take up hyaluronan during lung development. Developmental Biology, 1993, 155(2):324-36.
Penneys NS. CD44 expression in normal inflamed skin. Journal of Cutaneous Pathology, 1993, 20(3):250-3.
Messadi DV; Bertolami CN. CD44 hyaluronan expression in human cutaneous scar fibroblasts. American Journal of Pathology, 1993, 142(4):1041-9.
Johnson BA; Haines GK; Harlow LA; Koch AE. Adhesion molecule expression in human synovial tissue. Arthritis Rheumatism, 1993, 36:137-46.
Joensuu H; Klemi PJ; Toikkanen S; Jalkanen S. Glycoprotein CD44 expression its association with survival in breast cancer. American Journal of Pathology, 1993, 143(3):867-74.
Heider KH; Dammrich J; Skroch-Angel P; Muller-Hermelink HK; Vollmers HP; Herrlich P; Ponta H. Differential expression of CD44 splice variants in intestinal- diffuse-type human gastric carcinomas normal gastric mucosa. Cancer Research, 1993, 53(18):4197-203.
Fukazawa H; Yoshida K; Ichinohasama R; Sawai T; Hiromatsu Y; Mori K; Kikuchi K; Aizawa Y; Abe K; Wall JR. Expression of the Hermes-1 (CD44) ICAM-1 (CD54) molecule on the surface of thyroid cells from patients with Graves' disease. Thyroid, 1993, 3(4):285-9.
East JA; Mitchell SD; Hart IR. Expression function of the CD44 glycoprotein in melanoma cell lines. Melanoma Research, 1993, 3(5):341-6.
Aho R; Ekfors T; Haltia M; Kalimo H. Pathogenesis of primary central nervous system lymphoma: invasion of malignant lymphoid cells into within the brain parenchyme. Acta Neuropathologica, 1993, 86(1):71-6.
Abbasi AM; Chester KA; Talbot IC; Macpherson AS; Boxer G; Forbes A; Malcolm AD; Begent RH. CD44 is associated with proliferation in normal neoplastic human colorectal epithelial cells. European Journal of Cancer, 1993, 29A(14):1995-2002.
Penno MB; August JT; Baylin SB; Mabry M; Linnoila RI; Lee VS; Croteau D; Yang XL; Rosada C. Expression of CD44 in human lung tumors. Cancer Research, 1994, 54(5):1381-7.
Hughes DE; Salter DM; Simpson R. CD44 expression in human bone: a novel marker of osteocytic differentiation. Journal of Bone Mineral Research, 1994, 9(1):39-44.
Chaudhry A; Gobl A; Eriksson B; Skogseid B; Oberg K. Different splice variants of CD44 are expressed in gastrinomas but not in other subtypes of endocrine pancreatic tumors. Cancer Research, 1994, 54(4):981-6.

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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Positive Controls

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
Other Positive Controls Tonsil, HUVEC, HeLa cells

Lysates (3)

Name	Catalog#	Reactivity	Applications	Images
CD44 Lysate	H00000960-T01	Hu	WB	(2)
CD44 Lysate	NBL1-08952		WB	(1)
CD44 Lysate	H00000960-T02		WB

Peptides and Proteins

Name	Catalog#	Reactivity	Applications	Images
CD44 Recombinant Protein	H00000960-P02	Hu	ELISA, WB	(1)
CD44 Recombinant Protein	H00000960-P01		ELISA, WB	(1)
CD44 Protein	H00000960-G01	Hu	Func
CD44 Protein	NBP1-94196	Hu

Primary Antibodies (80)

Name	Catalog#	Reactivity	Applications	Images	Publications
CD44 Antibody (8E2F3)	NBP1-47386	Hu, Mu	ELISA, IF, IHC-Fr, IHC-P, WB	(7)
CD44 Antibody	NBP1-31488	Hu	ICC, IF, IHC-P, WB	(6)
CD44 Antibody (IM7)	NBP1-41266	Ca, Eq, Fe, Hu, Mu	FACS, ICC, IHC-Fr, IHC-P, IP, WB
CD44 Antibody (EPR1013Y)	NB110-55700	Hu, Mu(-), Rt(-)	FACS, ICC, IHC-P, WB	(2)	(3)
CD44 Antibody	NBP1-84578	Hu	IF, IHC-P, WB	(3)
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RNAi (5)

Name	Catalog#	Reactivity	Images	Publications
CD44 RNAi	H00000960-R10	Hu	(1)	(4)
CD44 RNAi	H00000960-R07	Hu	(1)	(4)
CD44 RNAi	H00000960-R08	Hu	(1)	(4)
CD44 RNAi	H00000960-R06	Hu	(1)	(4)
CD44 RNAi	H00000960-R09	Hu	(1)	(4)

Secondary Antibodies

Name	Catalog#	Reactivity	Applications	Publications
Mouse IgG Antibody [Biotin]	NB720-B	Mu	ELISA, IHC-P, WB
Mouse IgG Antibody [HRP]	NB720-H	Mu	ELISA, ICC, IHC-P, WB
Mouse IgG Antibody [Alkaline Phosphatase]	NB720-AP	Mu	ELISA, ICC, IHC-P, WB
Mouse IgG Antibody [FITC]	NB720-F	Mu	ICC, IHC-P	(2)
Mouse IgG Antibody	NB120-7056	Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-)	ELISA, IHC-P, IP, WB
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CD44 in Context

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CD44 Antibody (NB120-16728)

CD44 Antibody (156-3C11)