CD36 Antibody (NB400-144)
CD36 Antibody
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Distributor Data...
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anti-CHDS7 antibody, anti-CD36 molecule (thrombospondin receptor) antibody, anti-platelet collagen receptor antibody, anti-leukocyte differentiation antigen cd36 antibody, anti-gpiv antibody, anti-gpiiib antibody, anti-glycoprotein iiib antibody, anti-cd36 antigen antibody, anti-Platelet glycoprotein IV antibody, anti-gpIV antibody, anti-GPIIIb antibody, anti-CD 36 antibody, anti-thrombospondin receptor antibody, anti-Synonyms GPIV antibody, anti-scarb3 antibody, anti-platelet glycoprotein iv antibody, anti-Platelet glycoprotein 4 antibody, anti-Platelet collagen receptor antibody, anti-pasiv antibody, anti-PAS-4 protein antibody, anti-pas4 protein antibody, anti-PAS IV antibody, anti-PAS 4 protein antibody, anti-Leukocyte differentiation antigen CD36 antibody, anti-GPIV antibody, anti-GPIIIB antibody, anti-gp4 antibody, anti-gp3b antibody, anti-Glycoprotein IIIb antibody, anti-fatty acid translocase antibody, anti-FAT antibody, anti-CD36 molecule antibody, anti-CD36 antigen antibody |
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CD36 Antibody Summary: | | Gene: | CD36 |
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| Purity: | Affinity purified |
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| Host: | Rabbit |
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| | Specificity: | CD36 |
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| Publications: | Antibody has been mentioned in at least 5 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional CD36 Products CD36 Antibody Details: | Immunogen: | A synthetic peptide mapping to a region of human CD36 between residues 100-200. |
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| Localization: | Integral membrane |
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Reacts with human, bovine, mouse and monkey. 95% sequence identity with rat proteins. |
| Uses: | This CD36 antibody may be used for Immunohistochemistry and Western blot analysis, where a band is seen ~75-80 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. This antibody is also useful for immunocytochemistry/immunofluorescence. |
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| Dilutions: | Immunocytochemistry 1:50-1:200, Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:200-1:400, Western Blot 1:500-1:2000 |
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| TMW: | 53 kDa |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 1.0 mg/ml |
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| Positive Controls: | 2 Positive Controls |
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| Conjugated Antibodies: | 6 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | Tris-citrate / phosphate [pH 7-8] |
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| Preservative: | 0.09% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: CD36 is a cell surface class B scavenger receptor. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It may function as a cell adhesion molecule and directly mediates cyto- adherence of Plasmodium falciparum parasitized erythrocytes. CD36 binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport.
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Images (3) Reviews (1) Related Products (68) Exploring CD36 Working with CD36
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with CD36
CD36 Antibody Images (3) Western Blot: CD36 antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure. |  Immunocytochemistry: CD36 Antibody [NB400-144] - Detection of CD36 (Green) in HepG2 cells using NB400-144. Nuclei (Blue) were counterstained using Hoechst 33258, |  Immunohistochemistry: CD36 antibody [NB400-144] - Staining of human adipocytes using NB400-144. |
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Reviewed by:
Anonymous 2/9/2011
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Application:
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Western Blot
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Sample Tested:
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Whole tissue lysate ApoE murine aorta
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Sample Loaded:
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20ug
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Species:
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Mouse
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Sample Pretreated:
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Fed a cholesterol diet
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Lot:
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C2
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Results:
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Excellent antibody, works very well on both macrophages and in vivo murine aorta
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Blocking Buffer:
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5% milk in TBS-T
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Blocking Time:
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2 hours
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Blocking Temperature:
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room temperature Celsius
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Dilution Ratio:
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1:1000
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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5% milk in TBS-T
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Description:
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goat anti-rabbit
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Manufacturer Catalog #:
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sc-2004 Santa Cruz
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Dilution Ratio:
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1:10000
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Method:
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Supersignal Pierce
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Exposure Time:
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10 mins
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Positive Control:
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none
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Negative Control:
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none
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Did you observe bands?:
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Yes
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Specific Bands:
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75kDa kDa
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Non-specific Bands:
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none kDa
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Additional Comments:
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Excellent antibody
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Publications (5)CD36 Antibody Product Specific:- Lee JH, Wada T, Febbraio M, et al. A novel role for the dioxin receptor in fatty acid metabolism and hepatic steatosis. Gastroenterology. 2010 Aug;139(2):653-63. [PMID: 20303349]
- Marecki JC, Ronis MJ, Shankar K, Badger TM. Hyperinsulinemia and ectopic fat deposition can develop in the face of hyperadiponectinemia in young obese rats.J Nutr Biochem. 2011 Feb;22(2):142-52.[PMID: 20435456]
more... - Hoppmann S, Steinbach J, Pietzsch J.Scavenger receptors are associated with cellular interactions of S100A12 in vitro and in vivo.Int J Biochem Cell Biol. 2010 May;42(5):651-61. [PMID: 20025991]
- Chabowski A, Zmijewska M, Gorski J, et al. IL-6 deficiency increases fatty acid transporters and intramuscular lipid content in red but not white skeletal muscle. J Physiol Pharmacol. 2008 Dec;59 Suppl 7:105-17. [PMID: 19258660] (WB, Mouse)
- Tagliani E, Guermonprez P, Sepúlveda J, et al. Selection of an antibody library identifies a pathway to induce immunity by targeting CD36 on steady-state CD8 alpha+ dendritic cells. J Immunol. 2008 Mar 1;180(5):3201-9. [PMID: 18292544] (WB, Mouse)
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Jump to: Conjugated Antibodies, Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| CD36 Antibody [Biotin] | NB400-144B | Bv, Hu, Mk, Mu, Rt | IHC, WB | (2) | | | | | CD36 Antibody [HRP] | NB400-144H | Bv, Hu, Mk, Mu, Rt | IHC, WB | (2) | | | | | CD36 Antibody [HiLyte Fluor 488] | NB400-144L | Bv, Hu, Mk, Mu, Rt | IHC, WB | (2) | | | | | CD36 Antibody [DyLight 488] | NB400-144G | Bv, Hu, Mk, Mu, Rt | IHC, WB | | | | | | CD36 Antibody [DyLight 550] | NB400-144R | Bv, Hu, Mk, Mu, Rt | IHC, WB | | | | |
Positive ControlsLysates (2)Peptides and ProteinsPrimary Antibodies (48)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| CD36 Antibody (D-2712) | NB110-59724 | Hu(-), Mu, Rt | IHC, IHC-P, IP, WB | (2) | (2) | | | | CD36 Antibody | NB400-145 | Hu | ICC, IF, WB | (2) | (2) | | | | CD36 Antibody (mAb FA6-152) | NBP1-60151 | Hu | FACS, Func, IA, IF, IHC-Fr, IP | | (5) | | | | CD36 Antibody (FA6-152) | NBP1-60152 | Hu | FACS, Func, IA, IF, IHC-Fr, IP | | (5) | | | | CD36 Antibody (FA6-152) | NB600-1423 | Hu | FACS, Func, IA, IF, IHC-Fr, IP | | (3) | | | | ... and 43 more. See All » |
RNAi (3)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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