Home » Products » Primary Antibodies » CD36 Antibody (NB110-59724) (0.1 ml)
 

CD36 Antibody (NB110-59724)

CD36 Antibody (D-2712)

100% Guarantee This product is fully covered under the Novus guarantee+ Policy. Click here for details.

Distributor Data...

Loading Ajax
Loading...
Loading...
(sample size available)
 
Bulk Request
(see alternate names)
CD36 Antibody (D-2712) Summary:
Species:Hu(-), Mu, Rt
Applications:IHC, IHC-P, IP, WB
Clonality:Monoclonal
Gene:CD36
Purity:Protein G purified
Host:Mouse
Specificity:This is specific for CD36.
Publications:Antibody has been mentioned in at least 2 Publications.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
View Additional CD36 Products
LysatesPeptides and Proteins
Primary AntibodiesRNAi
 
» View all CD36 Related Products
CD36 Antibody (D-2712) Details:
Clone:D-2712
Isotype:IgA Kappa
Immunogen:Adenovirus expressing recombinant mouse CD36.
Localization:Membrane
Species Reactivity:

This antibody reacts with mouse and rat CD36 proteins. It is not reactive with human protein. Other species have not been tested.

Applications:
Uses:By Western blot a band is seen at ~70-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW, as seen in Western blot, is most likely due to the heavy glycosylation and palmitoylation of this protein. This clone has also been used to immunoprecipitate mouse CD36. This antibody is also useful for immunohistochemistry.
Dilutions:immunohistochemistry 0.5-1.0 ug/ml, immunoprecipitation , Western Blot 1:500, Immunohistochemistry-Paraffin 1:100-1:250
Unit Size:0.1 ml (also available: 0.025 ml (sample size))
Concentration:0.9 mg/ml
Positive Controls:3 Positive Controls
Conjugated Antibodies:5 Conjugated Antibodies
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:Tris-glycine, 150mM NaCl
Preservative:0.05% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: CD36
Entrez948 (Human)
12491 (Mouse)
29184 (Rat)
Q08857 (Mouse)
 
Background:

CD36 is a cell surface class B scavenger receptor. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signalling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cyto adherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport.
Images (2)
  • Detection of CD36 in mouse adipose lysate using NB110-59724.
  • Staining of CD36 in mouse liver using NB110-59724.

See all images...

Reviews (1)

Full StarFull StarFull StarEmpty StarEmpty Star

Anonymous on 11/28/2011

See all the detailed reviews
Review this product for a reward

Related Products (68)

2 Lysates

3 Peptides and Proteins

60 Primary Antibodies

3 RNAi

Secondary Antibodies

Exploring CD36

8 Related Research Areas

Working with CD36

2 Publications

1 Reviews from researchers like you

1 Protocols and Procedures
Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question

Working with CD36

CD36 Antibody (D-2712) Images (2)
Detection of CD36 in mouse adipose lysate using NB110-59724.Staining of CD36 in mouse liver using NB110-59724.
Reviews (1)  Have you used this product? Review this product to earn Novus Rewards.
Full StarFull StarFull StarEmpty StarEmpty Star

Reviewed by:
Anonymous 11/28/2011

Rat Liver CD36(novus biologicals) 1Ab 1:1000 5%milk ON @ 4 C; 2Ab GAM 1:40,000 5% milk 1 hr @RT
Application: Western Blot
Sample Tested: Liver whole tissue homogenate
Sample Loaded: 50ug
Species: Rat
Results: This ab seems to work well when compared to the observed molecular weight(~70-80 kDa) found in product insert. Fairly high non-specific binding and a little background, but nice strong band @ observed molecular weight. Tissue processed using Strom et al protocol
Blocking Buffer: 5%milk in TBST
Blocking Time: 1hr
Blocking Temperature: RT
Dilution Ratio: 1:1000
Incubation Time: overnight
Incubation Temperature: 4 Celsius
Incubation Dilution Buffer: 5% milk in TBST
Description: GAM-HRP BioRad
Manufacturer Catalog #: Bio Rad 170-6516
Dilution Ratio: 1:40,000
Method: Biorad Chemidoc XRS+ molecular imager, Western C chemiluminescent substrate
Exposure Time: 30 min
Did you observe bands?: Yes
Specific Bands: ~80 kDa kDa
Non-specific Bands: ~50 and 25 kDa kDa
 
Publications (2)

CD36 Antibody (D-2712) Product Specific:

  1. Febbraio M, et al. Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice. J Clin Invest. 2000 Apr;105(8):1049-56.
  2. During A, Doraiswamy S, Harrison EH. Xanthophylls are preferentially taken up compared with beta-carotene by retinal cells via a SRBI-dependent mechanism. J Lipid Res. 2008 Aug;49(8):1715-24. Epub 2008 Apr 19.
Protocols

Product Specific (1)


All General Protocols

Ask a Scientist

Can't find an answer to your question about CD36? Ask us by using live chat or by submitting your questions below:

 
Jump to: Conjugated Antibodies, Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

Related Products

Conjugated Antibodies
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
CD36 Antibody (D-2712) [DyLight 488]NB110-59724GHu(-), Mu, RtIHC, IHC-P, IP, WB

CD36 Antibody (D-2712) [DyLight 550]NB110-59724RHu(-), Mu, RtIHC, IHC-P, IP, WB

CD36 Antibody (D-2712) [DyLight 650]NB110-59724CHu(-), Mu, RtIHC, IHC-P, IP, WB

CD36 Antibody (D-2712) [HRP]NB110-59724HHu(-), Mu, RtIHC, IHC-P, IP, WB

CD36 Antibody (D-2712) [Biotin]NB110-59724BHu(-), Mu, RtIHC, IHC-P, IP, WB

Positive Controls
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Whole Normal Adipose LysateNB820-59655MuIP, WB

CD36 LysateNBL1-08939WB(1)

Other Positive Controls
  • Mouse adipose tissue lysate
Lysates (2)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
CD36 LysateNBL1-08940WB(1)

CD36 LysateNBL1-08939WB(1)

Peptides and Proteins
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
CD36 PeptideNB400-144PEP

CD36 PeptideNB400-145PEP

CD36 Recombinant ProteinH00000948-P02HuELISA, WB(1)

Primary Antibodies (60)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
CD36 AntibodyNB400-144Bv, Hu, Mk, Mu, RtICC, IF, IHC-P, WB(3)(5)

CD36 AntibodyNB400-145HuICC, IF, WB(2)(2)

CD36 Antibody (mAb FA6-152)NBP1-60151HuFACS, Func, IA, IF, IHC-Fr, IP(5)

CD36 Antibody (FA6-152)NBP1-60152HuFACS, Func, IA, IF, IHC-Fr, IP(5)

CD36 Antibody (FA6-152)NB600-1423HuFACS, Func, IA, IF, IHC-Fr, IP(3)

... and 55 more. See All »
RNAi (3)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
CD36 RNAiH00000948-R04Hu(1)(4)

CD36 RNAiH00000948-R06Hu(1)(4)

CD36 RNAiH00000948-R05Hu(1)(4)

Secondary Antibodies
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Mouse IgG Antibody [Biotin]NB720-BMuELISA, IHC-P, WB

Mouse IgG Antibody [HRP]NB720-HMuELISA, ICC, IHC-P, WB

Mouse IgG Antibody [Alkaline Phosphatase]NB720-APMuELISA, ICC, IHC-P, WB

Mouse IgG Antibody [FITC]NB720-FMuICC, IHC-P(2)

Mouse IgG AntibodyNB120-7056Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-)ELISA, IHC-P, IP, WB

See All »
Jump to: Novus Explorer, Research Areas

CD36 in Context

Research Areas related to CD36 (8):
Novus Explorer for CD36 gene
Try our bioinformatic tool that shows the relationships between CD36 and other genes, diseases, pathways and post-translational modifications.