CD3 Antibody (APA1/1) Summary:

Species:	Hu, Mu
Applications:	FACS, ICC, IHC-Fr, IP, WB
Clonality:	Monoclonal

Gene:	CD3E
Purity:	Protein A purified
Host:	Mouse

Specificity:	The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
Publications:	Antibody has at least 1 Background Reference.

Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.

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CD3 Antibody (APA1/1) Details:

Clone:	APA1/1
Isotype:	IgG
Immunogen:	Purified human CD3 proteins isolated from thymus

Species Reactivity:

Human and Mouse.

Applications:

Uses:	This antibody is useful in Flow Cytometry, Immunoprecipitation, Western Blotting, Immunohistochemistry-frozen and Immunocytochemistry
Dilutions:	Flow Cytometry, ImmunoPrecipitation 1:50, Western Blot 1:1000, Immunohistochemistry-Frozen 1:200, Immunocytochemistry
Unit Size:	0.1 mg
Concentration:	1.0 mg/ml

Packaging:

Storage:	Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:	PBS [pH 7.4]
Preservative:	15mM Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: CD3E

Entrez	916 (Human)
	12501 (Mouse)

Background:

CD3 epsilon is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3 epsilon, one CD3 gamma, one CD3 delta, one CD3 zeta (CD247), and a T-cell receptor ( alpha/ beta or gamma/ delta) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. The UCHT1 antibody is able to induce T cell activation.

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Publications (1)

CD3 Antibody (APA1/1) Background References:

*Alarcón B, Ley SC, Sánchez-Madrid F, Blumberg RS, Ju ST, Fresno M, Terhorst C: The CD3-gamma and CD3-delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes. EMBO J. 1991 Apr;10(4):903-12. *Wong AP, Dutly AE, Sacher A, Lee H, Hwang DM, Liu M, Keshavjee S, Hu J, Waddell TK: Targeted cell replacement with bone marrow cells for airway epithelial regeneration. Am J Physiol Lung Cell Mol Physiol. 2007 Sep;293(3):L740-52. *Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J Immunol. 2003 Jun 15;170(12):5947-55. *Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency. N Engl J Med. 2006 May 4;354(18):1913-21. *Tailor P, Tsai S, Shameli A, Serra P, Wang J, Robbins S, Nagata M, Szymczak-Workman AL, Vignali DA, Santamaria P: The proline-rich sequence of CD3epsilon as an amplifier of low-avidity TCR signaling. J Immunol. 2008 Jul 1;181(1):243-55. *Delgado P, Fernández E, Dave V, Kappes D, Alarcón B: CD3delta couples T-cell receptor signalling to ERK activation and thymocyte positive selection. Nature. 2000 Jul 27;406(6794):426-30. *Risueño RM, Gil D, Fernández E, Sánchez-Madrid F, Alarcón B: Ligand-induced conformational change in the T-cell receptor associated with productive immune synapses. Blood. 2005 Jul 15;106(2):601-8. *Gil D, Schrum AG, Alarcón B, Palmer E: T cell receptor engagement by peptide-MHC ligands induces a conformational change in the CD3 complex of thymocytes. J Exp Med. 2005 Feb 21;201(4):517-22. *Risueño RM, van Santen HM, Alarcón B: A conformational change senses the strength of T cell receptor-ligand interaction during thymic selection. Proc Natl Acad Sci U S A. 2006 Jun 20;103(25):9625-30.

Protocols

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Flow Cytometry

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