CD11b Antibody Summary:

Species:	Bv, Hu, Mu, Rt
Applications:	ICC, IF, IHC-P, WB
Clonality:	Polyclonal

Gene:	ITGAM
Purity:	Peptide affinity purified
Host:	Rabbit

Specificity:	CD11b
Publications:	Antibody has been mentioned in at least 3 Publications.

Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.

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CD11b Antibody Details:

Immunogen:	A synthetic peptide made to an internal region (within residues 250-350) of the mouse CD11b protein. [Swiss-Prot# P05555]
Localization:	Monocytes and granulocytes.
Marker:	Microglia Marker

Species Reactivity:

Reacts with human, mouse, rat and bovine. 88% sequence identity with sheep protein.

Applications:

Uses:	This CD11b antibody is useful for Western blot analysis, where a band is seen ~160 kDa, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Dilutions:	Immunocytochemistry 1:200, Immunofluorescence 1:200, Immunohistochemistry-Paraffin 1:400, Western Blot 2 ug/ml
Unit Size:	0.1 ml (also available: 0.025 ml (sample size))
Concentration:	1.13 mg/ml
Positive Controls:	2 Positive Controls
Conjugated Antibodies:	5 Conjugated Antibodies

Packaging:

Storage:	Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:	PBS, 30% Glycerol
Preservative:	0.1% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: ITGAM

Entrez	3684 (Human)
	16409 (Mouse)
	25021 (Rat)

Background:

CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.

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Working with CD11b

CD11b Antibody Images (3)

Immunohistochemistry: CD11b Antibody - Microglia Marker [NB110-89474] - IHC analysis of CD11b in human renal cancer using DAB with hematoxylin counterstain.	Western Blot: CD11b antibody [NB110-89474] - Detection of CD11b in RAW 264.7 whole cell lysates using NB110-89474.
Immunocytochemistry: CD11b Antibody [NB110-89474] - RAW 264.7 cells visualized with DyLight Fluor 488.

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Publications (3)

CD11b Antibody Product Specific:

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Protocols

Product Specific (1)

Protocol specific for CD11b Antibody (NB110-89474)

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Jump to: Conjugated Antibodies, Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

Related Products

Conjugated Antibodies

Name	Catalog#	Reactivity	Applications
CD11b Antibody [Biotin]	NB110-89474B	Bv, Hu, Mu, Rt	WB
CD11b Antibody [DyLight 650]	NB110-89474C	Bv, Hu, Mu, Rt	WB
CD11b Antibody [DyLight 488]	NB110-89474G	Bv, Hu, Mu, Rt	WB
CD11b Antibody [HRP]	NB110-89474H	Bv, Hu, Mu, Rt	WB
CD11b Antibody [DyLight 550]	NB110-89474R	Bv, Hu, Mu, Rt	WB

Positive Controls

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
Raw 264.7 Whole Cell Lysate	NBP1-30158		WB	(1)
Other Positive Controls RAW 264.7 cell lysate

Peptides and Proteins

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
CD11b Partial Recombinant Protein	H00003684-Q01	Hu	ELISA, WB	(1)

Primary Antibodies (53)

Name	Catalog#	Reactivity	Applications	Images	Publications
CD11b Antibody (M1/70.15)	NB600-1327	Hu, Mu, Rb	FACS, IF, IHC-Fr, IHC-P, IP	(2)	(13)
CD11b Antibody (M1/70)	NBP1-45013	Hu, Mk, Mu, Rb	FACS, Func, IHC-Fr, IP		(4)
CD11b Antibody (EP1345Y)	NB110-55684	Hu, Mu(-), Rt(-)	FACS, ICC, IHC-P, IP, WB	(2)	(2)
CD11b Antibody	NBP1-50231	Hu, Rt	FACS, IHC-Fr, IHC-P, IP	(1)
CD11b Antibody (Bear-1)	NBP1-42113	Hu	IF, IHC-Fr, IP
... and 48 more. See All »

RNAi (1)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
CD11b RNAi	H00003684-R01	Hu		(1)	(4)

Secondary Antibodies

Name	Catalog#	Reactivity	Applications	Images	Publications
Rabbit IgG Antibody [HRP]	NB730-H	Rb	ELISA, ICC, IHC-P, WB		(5)
Rabbit IgG Antibody [FITC]	NB730-F	Rb	ICC, IHC-P		(1)
Rabbit IgG Antibody [Biotin]	NB730-B	Rb	ELISA, ICC, IHC-P, WB
Rabbit IgG, H/L Chains Antibody [DyLight 488]	NBP1-72944	Rb	FLISA, IF, WB	(3)
Rabbit IgG Antibody [Alkaline Phosphatase]	NB730-AP	Rb	ELISA, ICC, IHC-P, WB		(1)
See All »

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CD11b in Context

Research Areas related to CD11b (13):

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CD11b Antibody (NB110-89474)

CD11b Antibody