CD11b Antibody (NBP1-30423)

CD11b Antibody (M1/70) [PE]

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CD11b Antibody (M1/70) [PE] Summary:
Species:Hu, Mu, Rb
Applications:FACS, Func, IHC-Fr, IP
Clonality:Monoclonal
Gene:ITGAM
Purity:IgG purified
Host:Rat
Specificity:The rat monoclonal antibody M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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CD11b Antibody (M1/70) [PE] Details:
Conjugate:PE
Clone:M1/70
Isotype:IgG2b
Immunogen:B10 mouse spleen cells enriched for T cells
Marker:Microglia Marker
Species Reactivity:

Cross reacts with Human, Non-Human Primates, Mouse and Rabbit.

Applications:
Uses:This antibody is useful in Flow Cytometry analysis.
Dilutions:Flow Cytometry, ImmunoPrecipitation 1:50, Immunohistochemistry-Frozen 1:200, Functional
Unit Size:0.1 mg
Concentration:0.1 mg/ml
Notes:
Store in the dark, avoid prolonged exposure to light.
Packaging:
Storage:Store at 4 °C. Do not freeze.
Buffer:PBS containing 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative:15mM Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: ITGAM
 
Background:

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

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Publications (1)

CD11b Antibody (M1/70) [PE] Background References:

  1. *Springer T, Galfrè G, Secher DS, Milstein C: Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978 Aug;8(8):539-51. *Ault KA, Springer TA: Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 1981 Jan;126(1):359-64. *Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983 Aug 1;158(2):586-602. *Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ: Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 1995 Mar;43(3):313-20. *Dembic Z, Schenck K, Bogen B: Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells. Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2697-702. *Welt FG, Edelman ER, Simon DI, Rogers C: Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries. Arterioscler Thromb Vasc Biol. 2000 Dec;20(12):2553-8. *Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC: Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol. 2002 Mar 15;168(6):3088-98. *Brickson S, Ji LL, Schell K, Olabisi R, St Pierre Schneider B, Best TM: M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury. J Appl Physiol. 2003 Sep;95(3):969-76. *Leon F, Contractor N, Fuss I, Marth T, Lahey E, Iwaki S, la Sala A, Hoffmann V, Strober W, Kelsall BL: Antibodies to complement receptor 3 treat established inflammation in murine models of colitis and a novel model of psoriasiform dermatitis. J Immunol. 2006 Nov 15;177(10):6974-82. *Takagi H, Numazaki M, Kajiwara T, Abe Y, Ishii M, Kato C, Kojima N: Cooperation of specific ICAM-3 grabbing nonintegrin-related 1 (SIGNR1) and complement receptor type 3 (CR3) in the uptake of oligomannose-coated liposomes by macrophages. Glycobiology. 2009 Mar;19(3):258-66.

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CD11b in Context


Research Areas related to CD11b (14):
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