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CD11b Antibody (NB100-77751)

CD11b Antibody (ICRF44)

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CD11b Antibody (ICRF44) Summary:
Species:Bb, Hu, Mk, Po
Applications:FACS, IF, IHC-Fr, IP
Clonality:Monoclonal
Gene:ITGAM
Purity:Immunogen affinity purified
Host:Mouse
Specificity:CD11b (ICRF44)
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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CD11b Antibody (ICRF44) Details:
Clone:ICRF44
Isotype:IgG1 Kappa
Immunogen:The immunogen was the full length version of the protein: CD11b (ICRF44)
Marker:Microglia Marker
Species Reactivity:

Human, Expected Cross-Reactivity based on immunogen sequence: Chimpanzee, Baboon, Cynomolgus, Rhesus, Swine (Pig, Porcine).

Applications:
Uses:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.
Dilutions:Flow Cytometry , immunofluorescence , Immunohistochemistry-Frozen
Unit Size:0.1 mg
Concentration:0.5 mg/ml
Positive Controls:1 Positive Controls
Conjugated Antibodies:1 Conjugated Antibodies
Packaging:
Storage:Store at 4 °C. Do not freeze.
Buffer:PBS [pH 7.2]
Preservative:0.09% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: ITGAM
Entrez3684 (Human)
 
Background:

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as alphaM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin beta2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and Factor X. The ICRF44 antibody inhibits granulocyte activation in response to fMLP.
Images (1)
  • Flow Cytometry: CD11b Antibody (ICRF44) [NB100-77751] - Human peripheral blood granulocytes stained with purified ICRF44, followed by anti-mouse IgGs FITC

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Working with CD11b

CD11b Antibody (ICRF44) Images (1)
Flow Cytometry: CD11b Antibody (ICRF44) [NB100-77751] - Human peripheral blood granulocytes stained with purified ICRF44, followed by anti-mouse IgGs FITC
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Publications (1)

CD11b Antibody (ICRF44) Background References:

  1. Stewart, M., et al., 1995. Curr. Opin. Cell Biol. 7:690.
Protocols
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