CD11b Antibody (EPR1344) 0.1 ml Microglia Marker, Macrophage Marker

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Ordering Information: NBP1-42075

  • Catalog Number
    NBP1-42075
  • Displayed Format
    Unconjugated
  • Size(s)
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    CD11b Antibody (EPR1344) Summary

    Species Human, Mouse, Rat (Negative)
    Tested Applications WB, Flow, ICC/IF, IHC, IHC-P
    Clonality
    Monoclonal
    Host
    Rabbit
    Gene
    ITGAM
    Purity
    Protein A purified
    Guarantee Plus
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    CD11b Antibody (EPR1344) Details

    Clone
    EPR1344
    Isotype
    IgG
    Marker
    Microglia Marker, Macrophage Marker
    Immunogen
    A synthetic peptide corresponding to residues in human CD11b was used as an immunogen.

    Species Reactivity

    For Mouse this antibody is recommended for Western Blot only.
    Reviewed Species Read 3 rated 4
    using NBP1-42075 in the following species:

    Applications/Dilutions

    Dilutions
    • Western Blot 1:1000-5000
    • Flow Cytometry 1:100
    • Immunocytochemistry/Immunofluorescence 1:100-250
    • Immunohistochemistry 1:10-1:500
    • Immunohistochemistry-Paraffin 1:100-250
    Application Notes
    In Western blot this antibody detects a band at approximately 170 kDa.
    Molecular Weight
    127 kDa
    Reviewed Applications
    Read 3 Review rated 4
    using
    NBP1-42075 in the following applications:

    Contact Information

    Product PDFs

    Reviews

    Average Rating: 4
    Recent Reviews
    WB Tested In Mouse
    Reviewed On: 02/28/2014
    Rated: 4

    CD11b NBP1-42075
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    Publications

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    Notes

    Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Packaging, Storage & Formulations

    Storage
    Store at -20C. Avoid freeze-thaw cycles.
    Buffer
    PBS 59%, Glycerol 40%, BSA 0.05%, pH: 7.40
    Unit Size
    0.1 ml
    Concentration
    Please see the vial label for concentration. If unlisted please contact technical services.
    Preservative
    0.01% Sodium Azide
    Limitations
    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

    Bioinformatics

    Gene Symbol ITGAM
    Entrez
    Uniprot

    Alternate Names for CD11b Antibody (EPR1344)

    • antigen CD11b (p170)
    • CD11 antigen-like family member B
    • CD11b antigen
    • CD11b
    • CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide)
    • Cell surface glycoprotein MAC-1 subunit alpha
    • CR-3 alpha chain
    • CR3AMGC117044
    • Integrin alpha M
    • integrin alpha-M
    • integrin, alpha M (complement component 3 receptor 3 subunit)
    • ITGAM
    • Leukocyte adhesion receptor MO1
    • MAC-1
    • MAC1A
    • macrophage antigen alpha polypeptide
    • MO1A
    • neutrophil adherence receptor alpha-M subunit
    • Neutrophil adherence receptor
    • SLEB6
    Show More

    Related Products by Gene

    Background

    CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

    Reviews for CD11b Antibody (NBP1-42075) (3) 43

    Read what people are saying who have used CD11b Antibody (NBP1-42075).

    We have 3 reviews tested in 2 species: Human, Mouse.
    We have 3 reviews tested in 2: Western Blot, Immunofluorescence.
    Average Rating: 4
    (Based on 3 reviews)

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    Sort 3 reviews for CD11b Antibody (NBP1-42075) by:
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    (2)
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    Images Ratings Applications Species Reviewed By Date Details
    Western Blot CD11b NBP1-42075
    Click to Enlarge
    Very Good
    WB Mouse Anonymous 02/28/2014
    Show

    Summary

    ApplicationWestern Blot
    Sample TestedMouse peripheral nerve and ganglia
    SpeciesMouse
    LotYG0420405C

    Blocking

    Blocking Details5% BSA for 1 hour at room temperature

    Primary Anitbody

    Dilution Ratio1:1000 in 5% BSA overnight at 4 degrees C

    Secondary Antibody

    Secondary Manufacturer Cat#711-036-152
    Secondary Concentration1:2000

    Details

    Detection NotesECL detection
    Western Blot CD11b NBP1-42075
    Click to Enlarge
    Very Good
    WB Mouse Anonymous 02/28/2014
    Show

    Summary

    ApplicationWestern Blot
    Sample TestedMouse sciatic nerves
    SpeciesMouse
    LotYG0420405C
    Immunofluorescence CD11b NBP1-42075
    Click to Enlarge
    Very Good
    IF Human Anonymous 10/03/2013
    Show

    Summary

    ApplicationImmunofluorescence
    SpeciesHuman
    Special ApplicationsThe cells were washed twice in PBS and fixed for 5 min at room temperature in 4% formaldehyde in PBS (v/v). Fixed cells were incubated for 30 min with 10% bovine serum albumin (BSA) in PBS (w/v) to block unspecific bindings. After blocking, cells were incubated with primary and secondary antibodies for 60 min each at room temperature in the dark. Antibodies were diluted in immunostain enhancer (Thermo Scientific, Belgium) as follows: rabbi anti-human CD11b 1:200 (clone EPR1344; Novus Biologicals UK), goat anti-rabbit dylight 488 1:2000 (AS09 633; Agrisera, Vännas, Sweden). Nuclei were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) and cellular membranes were stained with cell mask deep red (C10046; Invitrogen, Leusden, the Netherlands).
    Pub Med ID23890538
    FileView PDF

    Publications for CD11b Antibody (NBP1-42075) (0)

    There are no publications for CD11b Antibody (NBP1-42075).
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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol
    ICC/IF Video Protocol

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    FAQs for CD11b (7)

    1. I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
      • I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
    2. What types of cells can this CD11b antibody be used as a marker for?
      • A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
    3. Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 
      • This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
    4. Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
      • I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
    5. We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
      • We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
    6. The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
      • The protocol is referencting the PIER method using Proteinase K as the enzyme.
    7. I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
      • We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.

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    Bioinformatics Tool for CD11b

    Discover related pathways, diseases and genes to CD11b. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
    Bioinformatics Tool
    Vizit™, under license from BioVista Inc.

    Blogs on CD11b

    Check out the latest blog posts on CD11b.
    CD11b - More than a microglial marker
    The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the...  Read full blog post.

    CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
    Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

    Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases
    Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This...  Read full blog post.

    CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
    The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

    The CD11b Antibody: A Marker for Microglial Cells
    Microglia are the resident macrophages of the central nervous system, and the first line of immune defense. Pioneering antibody research in the 1990's identified the Integrin beta 2 protein (also called ITGB2, complement receptor 3, CR3, CD18, and Mac...  Read full blog post.

    CD11b Expression, Leukocyte Adhesion and the Innate Immune System
    CD11b is an integrin family member which pairs with CD18 to form the CR3 heterodimer. CD11b is expressed on the surface of many leukocytes including monocytes, neutrophils, natural killer cells, granulocytes and macrophages, as well as on 8% of spleen...  Read full blog post.