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CCR2 Antibody (NB100-718)

CCR2 Antibody

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CCR2 Antibody Summary:
Species:Hu
Applications:ELISA, ICC, IHC-P, WB
Clonality:Polyclonal
Gene:CCR2
Purity:Immunogen affinity purified
Host:Goat
Specificity:Peptide sequence is < 50 % identical to other human chemokine receptors in this region.
Publications:Antibody has been mentioned in at least 1 Publication.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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CCR2 Antibody Details:
Isotype:IgG
Immunogen:Synthetic peptide: CGEEVTTFFDYDYGAPCHKFDVKQ (Human).
Localization:Cell Membrane
Species Reactivity:

Cross-reacts with Human. Not yet tested in other species.

Applications:
Uses:ELISA: Use at a dilution of 1/60,000. ICC: Use at a dilution of 1/400. IHC-P: Use at a dilution of 1/200. Antigen retrieval is not essential but may optimize staining. WB: Use at a dilution of 1/1,000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Dilutions:ELISA 1/60000, Western Blot 1/1000, Immunocytochemistry 1/400, Immunohistochemistry-Paraffin 1/200
Unit Size:0.1 mg
Concentration:1.0 mg/ml
Positive Controls:4 Positive Controls
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:PBS, 1mg/ml BSA
Preservative:0.1% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: CCR2
Entrez729230 (Human)
 
Background:

CCR2, or C C chemokine receptor type 2, is a receptor for several monocyte chemo attractant proteins (MCP1, MCP3, MCP4) which specifically mediate monocyte chemotaxis. CCR2 transduces such signals by increasing the intracellular level of calcium ions. For example, MCP1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. CCR2 is also an alternative coreceptor with CD4 for HIV1 infection. CCR2 has two isoforms and has been reported to be expressed in a wide variety of tissues, including blood, brain, heart, kidney, liver, lung, ovary, pancreas, spinal cord, spleen and thymus.
Images (1)
  • Immunohistochemistry: CCR 2 Antibody [NB100-718] - Immunohistochemistry on paraffin section of human spleen

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CCR2 Antibody Images (1)
Immunohistochemistry: CCR 2 Antibody [NB100-718] - Immunohistochemistry on paraffin section of human spleen
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Publications (1)

CCR2 Antibody Product Specific:

  1. Sakamoto T, Ishibashi T, Sakamoto N, et al. Endogenous NO blockade enhances tissue factor expression via increased Ca2+ influx through MCP-1 in endothelial cells by monocyte adhesion. Arterioscler Thromb Vasc Biol. 2005 Sep;25(9): 2005-11. [PMID 16020745] (Human, WB)
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