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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
ICC/IF Video Protocol
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FAQs for Bmi1 (2)
- Do you have any recommendation for a blocking buffer for use of a primary antibody in Western Blot?
- Could you please provide information on how to find positive controls for your primary antibodies?
Do you have any recommendation for a blocking buffer for use of a primary antibody in Western Blot?
The blocking buffer that is often employed in our Western Blot QC validation is either 3% BSA, or 5% NFDM (Non-fat Dry Milk). In certain cases a mixture of both BSA and NFDM will be employed. Ensure that BSA is used as the blocking buffer for antibodies that target phosphorylated post translational modifications. This is to ensure that the antibody does not non-specifically bind the casein found in NFDM.
Could you please provide information on how to find positive controls for your primary antibodies?
The images on our datasheets will provide detailed information on potential positive control cells and/or tissues, which have been previously tested by the lab for antibody validation. We also provide a related products tab at the top of the datasheet, which will contain useful control information where available. In addition to these tools, we recommend checking tools such as www.uniprot.org or www.proteinatlas.org to determine tissues and cell lines that highly express your protein of interest.
Bioinformatics Tool for Bmi1
Discover related pathways, diseases and genes to Bmi1. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Diseases for Bmi1
Discover more about diseases related to Bmi1.
- Neoplasms
- Malignant Neoplasms
- Cell Transformation, Neoplastic
- Carcinoma
- Leukemia
- Malignant Paraganglionic Neoplasm
- Neoplasm Metastasis
- Lymphoma
- Malignant Neoplasm Of Breast
- Myeloid Leukemia
- Mammary Neoplasms
- Malignant Squamous Cell Neoplasm
- Brain Neoplasms
- Cell Invasion
- Tumorigenicity
- Carcinogenesis
- Leukemia, Myelocytic, Acute
- Tumor Growth
- Lung Neoplasms
- Neoplasm Invasiveness
Pathways for Bmi1
View related products by pathway.
- Senescence
- Cell Cycle
- Cell Proliferation
- Methylation
- Pathogenesis
- Cell Growth
- Oncogenesis
- Gene Silencing
- Cell Differentiation
- Cellular Senescence
- Localization
- Regeneration
- Rna Interference
- Cell Death
- Dna Methylation
- Aging
- Replicative Senescence
- Drug Resistance
- Dna Repair
- Stem Cell Maintenance
Research Areas for Bmi1
Find related products by research area.
PTMs for Bmi1
Learn more about PTMs related to Bmi1.
- Methylation
- Phosphorylation
- Ubiquitination
- Acetylation
- Demethylation
- Cleavage
- Deacetylation
- Reduction
- Dephosphorylation
- Sumoylation
Blogs on Bmi1
Check out the latest blog posts on Bmi1.
![Western Blot: Bmi1 Antibody [NBP1-87321] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/Bmi1 Antibody-Western Blot-NBP1-87321-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Bmi1 Antibody [NBP1-87321] Staining of human cell line A-431 shows positivity in nucleus.](http://images.novusbio.com/fullsize/Bmi1 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87321-img0004.jpg)
![Immunohistochemistry-Paraffin: Bmi1 Antibody [NBP1-87321] - Staining of human kidney shows strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/Bmi1 Antibody-Immunohistochemistry-Paraffin-NBP1-87321-img0002.jpg)
-Western Blot-NBP1-96082-img0003.jpg "Western Blot: KMT6/EZH2 [p Thr487] Antibody (EPR1410) [NBP1-96082] - HeLa cell lysates. Cells were either (A) untreated or (B) treated with Lambda Phosphatase.")
-Immunohistochemistry-Paraffin-NBP1-96082-img0004.jpg "Immunohistochemistry-Paraffin: KMT6/EZH2 [p Thr487] Antibody (EPR1410) [NBP1-96082] Human prostatic tissue.")
-Western Blot-NB600-302-img0005.jpg "Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.")
-Immunocytochemistry-Immunofluorescence-NB600-302-img0004.jpg "Immunocytochemistry/Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells stained NB600-302 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).")
-Western Blot-NB110-57434-img0004.jpg "Western Blot: PRC1 Antibody (EP1513Y) [NB110-57434] HeLa cells.")
-Immunohistochemistry-NB110-57434-img0001.jpg "Immunohistochemistry: PRC1 Antibody (EP1513Y) [NB110-57434] - Immunohistochemical staining of paraffin-embedded human cervical carcinoma using anti-PRC1 (cat. #110-57434).")
-Western-Blot-NB200-103-img0001.jpg "Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.")
-Western-Blot-NB500-303-img0004.jpg "Western Blot: p21 Antibody (WA-1) [NB500-303] - WB analysis of p21 in horse skin cells. Image courtesy of prodcut review submitted by Karine Deschene.")
-Immunocytochemistry-Immunofluorescence-NB500-303-img0003.jpg "Immunocytochemistry/Immunofluorescence: p21 Antibody (WA-1) [NB500-303] - IF analysis of p21 in horse skin cells. Image courtesy of prodcut review submitted by Karine Deschene.")
-Western Blot-NB100-317-img0011.jpg "Western Blot: Telomerase reverse transcriptase Antibody (2C4) [NB100-317] - 1:500 dilution on MJ90 cells."){kind=spacer}
-Immunocytochemistry-Immunofluorescence-NB100-317-img0007.jpg "Immunocytochemistry/Immunofluorescence: Telomerase reverse transcriptase Antibody (2C4) [NB100-317] - Detection of Tert (Green) in HepG2 cells using NB100-317. Nuclei (Blue) are counterstained with Hoechst 33258."){kind=spacer}
-Western Blot-NB100-524-img0006.jpg "Western Blot: NOD2 Antibody (2D9) [NB100-524] - Detection of endogenous NOD2 in 50ug of HT29 cell lysate using NB 100-524. ECL detection in 15 minutes.")
-Western Blot-NB100-524-img0005.jpg "Western Blot: NOD2 Antibody (2D9) [NB100-524] - Detection of NOD2 in 20ug of NOD2 transfected 293T cell lysate using NB 100-524. ECL detection in 10 seconds.")
