Blimp-1 Antibody (3H2-E8) 0.1 ml Plasma Cell Marker

Blimp-1 NB600-235

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Catalog NumberNB600-235
  • FormUnconjugated
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Blimp-1 Antibody (3H2-E8) Summary

 
Species Human, Mouse, Porcine
Tested Applications WB, ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality Monoclonal
Host Mouse
Gene PRDM1
Purity Protein G purified
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Blimp-1 Antibody (3H2-E8) Details

 
Clone 3H2-E8
Isotype IgG1
Marker Plasma Cell Marker
Immunogen A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]
Localization Nuclear

Species Reactivity

 
Human, mouse and pig.
Reviewed Species Read 2 rated 3.5  using NB600-235 in the following species:
 
Publications Read 18 using NB600-235 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry 8 ug/ml where cells are used at 2*10^6/mL
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
Application Notes

This Blimp-1 (3H2-E8) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry on paraffin-embedded sections and Flow Cytometry. Immunohistochemistry-Frozen was reported in scientific literature. By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. This antibody does not immunoprecipitate. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.

Positive Controls

2 Positive Controls

Other Available
Formats
7C Labeled NB600-235V2
Alexa Fluor (R) 405 Labeled NB600-235AF405
Alexa Fluor (R) 488 Labeled NB600-235AF488
Alexa Fluor (R) 647 Labeled NB600-235AF647
Alexa Fluor (R) 700 Labeled NB600-235AF700
Allophycocyanin Labeled NB600-235APC
Biotin Labeled NB600-235B
DyLight 350 Labeled NB600-235UV
DyLight 405 Labeled NB600-235V
DyLight 405LS Labeled NB600-235V3
DyLight 488 Labeled NB600-235G
DyLight 550 Labeled NB600-235R
DyLight 650 Labeled NB600-235C
DyLight 680 Labeled NB600-235FR
DyLight 755 Labeled NB600-235IR
FITC Labeled NB600-235F
HRP Labeled NB600-235H
PE Labeled NB600-235PE
PerCP Labeled NB600-235PCP
Learn about Custom Antibody Labeling
Reviewed Applications
Read 1 review rated 4  using
NB600-235 in the following application:
Publications
Read 18 publications using
NB600-235 in the following applications:
  • WB7publications

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Reviews

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Publications

Blimp-1 Antibody (NB600-235) has been mentioned in at least 27 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

View all Blimp-1 Research Tools.

Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer Tris-glycine, 150 mM NaCl
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration 1.2 mg/ml
Preservative 0.1% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol PRDM1
Entrez
Uniprot

Alternate Names for Blimp-1

 
  • PRDI-binding factor-1
  • MGC118922
  • PR-domain zinc finger protein 1
  • PRDI-binding factor 1
  • PR domain zinc finger protein 1
  • PR domain-containing protein 1
  • beta-interferon gene positive-regulatory domain I binding factor
  • BLIMP-1
  • B-lymphocyte-induced maturation protein 1
  • Positive regulatory domain I-binding factor 1
  • Beta-interferon gene positive regulatory domain I-binding factor
  • BLIMP1MGC118925
  • PRDI-BF1MGC118924
  • MGC118923
  • PR domain containing 1, with ZNF domain

Related Products by Gene

Background

 
Blimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor which primarily controls the terminal differentiation of mature B cells to plasma cells, and also involves in macrophage as well as T-cells population homeostasis/differentiation. It is also called positive regulatory domain I-binding factor-1 (PRDI-BF1) or PR (PRDI-BF1-RIZ) domain zinc finger protein 1 (PRDM1) and its first human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-beta promoter leading to inhibition of virus-mediated IFN-beta production. Blimp-1 contains N-terminal PR/SET domain and five C2H2 zinc fingers located near its C-terminus that mediate DNA binding, nuclear import and recruitment of histone modifying enzymes, and these activities enable Blimp-1 for its ability to control cell-fate decisions in the embryo and govern tissue homeostasis in multiple cell types in the adult organism. Blimp-1 recruits chromatin-modifying enzymes including HDACs and methyltransferases, and some of its target genes include c-Myc, CIITA, Pax5, Spi-B, and Id3. Blimp-1 sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Deletion mutation of Blimp-1 is frequently observed in several tumors including lymphoid malignancies and has been proposed to be a candidate of tumor suppressor gene.