Blimp-1 Antibody (3H2-E8) 0.1 ml Plasma Cell Marker

Blimp-1 NB600-235

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Catalog NumberNB600-235
  • FormUnconjugated
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Blimp-1 Antibody (3H2-E8) Summary

 
Species Human, Mouse, Porcine
Tested Applications WB, ChIP, ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality Monoclonal
Host Mouse
Gene PRDM1
Purity Protein G purified
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Blimp-1 Antibody (3H2-E8) Details

 
Clone 3H2-E8
Isotype IgG1
Marker Plasma Cell Marker
Immunogen A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]
Localization Nuclear

Species Reactivity

 
Human, mouse and pig.
Reviewed Species Read 2 rated 3.5  using NB600-235 in the following species:
 
Publications Read Publications using NB600-235 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 1ug antibody / 10 ug protein
  • ELISA 1:100-1:2000
  • Flow Cytometry 8 ug/ml where cells are used at 2*10^6/mL
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
Application Notes

This Blimp-1 (3H2-E8) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation and Chromatin Immunoprecipitation applications. Immunohistochemistry-Frozen was reported in scientific literature. By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.

Positive Controls

2 Positive Controls

Other Available
Formats
7C Labeled NB600-235V2
Alexa Fluor (R) 405 Labeled NB600-235AF405
Alexa Fluor (R) 488 Labeled NB600-235AF488
Alexa Fluor (R) 647 Labeled NB600-235AF647
Alexa Fluor (R) 700 Labeled NB600-235AF700
Allophycocyanin Labeled NB600-235APC
Biotin Labeled NB600-235B
DyLight 350 Labeled NB600-235UV
DyLight 405 Labeled NB600-235V
DyLight 405LS Labeled NB600-235V3
DyLight 488 Labeled NB600-235G
DyLight 550 Labeled NB600-235R
DyLight 650 Labeled NB600-235C
DyLight 680 Labeled NB600-235FR
DyLight 755 Labeled NB600-235IR
FITC Labeled NB600-235F
HRP Labeled NB600-235H
PE Labeled NB600-235PE
PerCP Labeled NB600-235PCP
Learn about Custom Antibody Labeling
Reviewed Applications
Read 1 review rated 4  using
NB600-235 in the following application:
Publications
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NB600-235 in the following applications:

Product PDFs

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Publications

Blimp-1 Antibody (NB600-235) has been mentioned in at least 31 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

View all Blimp-1 Research Tools.

Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer Tris-glycine, 150 mM NaCl
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration 1.2 mg/ml
Preservative 0.1% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol PRDM1
Entrez
Uniprot

Alternate Names for Blimp-1

 
  • PRDI-binding factor-1
  • MGC118922
  • PR-domain zinc finger protein 1
  • PRDI-binding factor 1
  • PR domain zinc finger protein 1
  • PR domain-containing protein 1
  • beta-interferon gene positive-regulatory domain I binding factor
  • BLIMP-1
  • B-lymphocyte-induced maturation protein 1
  • Positive regulatory domain I-binding factor 1
  • Beta-interferon gene positive regulatory domain I-binding factor
  • BLIMP1MGC118925
  • PRDI-BF1MGC118924
  • MGC118923
  • PR domain containing 1, with ZNF domain

Related Products by Gene

Background

 
Blimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor which primarily controls the terminal differentiation of mature B cells to plasma cells, and also involves in macrophage as well as T-cells population homeostasis/differentiation. It is also called positive regulatory domain I-binding factor-1 (PRDI-BF1) or PR (PRDI-BF1-RIZ) domain zinc finger protein 1 (PRDM1) and its first human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-beta promoter leading to inhibition of virus-mediated IFN-beta production. Blimp-1 contains N-terminal PR/SET domain and five C2H2 zinc fingers located near its C-terminus that mediate DNA binding, nuclear import and recruitment of histone modifying enzymes, and these activities enable Blimp-1 for its ability to control cell-fate decisions in the embryo and govern tissue homeostasis in multiple cell types in the adult organism. Blimp-1 recruits chromatin-modifying enzymes including HDACs and methyltransferases, and some of its target genes include c-Myc, CIITA, Pax5, Spi-B, and Id3. Blimp-1 sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Deletion mutation of Blimp-1 is frequently observed in several tumors including lymphoid malignancies and has been proposed to be a candidate of tumor suppressor gene.