Beta-endorphin Antibody (NB120-10339)

Beta-endorphin Antibody

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Beta-endorphin Antibody Summary:
Species:Bv, Ch, Fe, Hu, Mk, Mu, Po, Rt
Applications:IF, IHC-Fr, IHC-P
Clonality:Polyclonal
Gene:POMC
Purity:Whole antisera
Host:Rabbit
Specificity:0.01% with beta lipotropin and 100% with beta-endorphin.
Publications:Antibody has at least 2 Background References.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Beta-endorphin Antibody Details:
Isotype:IgG
Immunogen:Antiserum to beta Endorphin was raised in rabbits by using the N-terminal portion of bovine beta Endorphin conjugated to thyroglobulin. (YGGFMTSKSQTPLVTLFKNAIIKNAYKKG E)
Localization:Secreted
Species Reactivity:

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Applications:
Uses:For slide-mounted tissue sections it is recommended to make working dilution for immunofluorescence histochemistry 15 µg/ml, whereas for avidin-biotin immunohistochemistry antiserum may be diluted to 5 µg/ml. Optimal dilutions/concentrations should be determined by the end user.
Dilutions:Immunofluorescence 15 μg/ml, Immunohistochemistry-Frozen 5 μg/ml, Immunohistochemistry-Paraffin
Unit Size:0.05 ml
Concentration:Lyoph
Positive Controls:1 Positive Controls
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:Whole Serum Lyophilized. Reconstitute to desired concentration in diH2O.
Preservative:0.1% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: POMC
Entrez5443 (Human)
18976 (Mouse)
24664 (Rat)
 
Background:

ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.

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Beta-endorphin Antibody Images (1)
Immunohistochemistry: Beta-endorphin Antibody [NB120-10339] - Detection of beta-Endorphin (dark-blue varicosities and processes) in rat pituitary (HRP-DAB-with Ni enhancement).
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Publications (2)

Beta-endorphin Antibody Background References:

  1. Harris, J. I. Structure of a melanocyte-stimulating hormone from the human pituitary gl. Nature 184:167-169 (1959).Chretien M et al. From beta-lipotropin to beta-endorphin 'pro-opio-melanocortin. Canad. J. Biochem. 57:1111-1121 (1979).
  2. LEE TH et al. On the structure of human corticotropin (adrenocorticotropic hormone). J Biol Chem 236:2970-4 (1961).

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Jump to: Positive Controls, Primary Antibodies, Secondary Antibodies

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Secondary Antibodies
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Rabbit IgG Antibody [HRP]NB730-HRbELISA, ICC, IHC-P, WB(5)

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Rabbit IgG, H/L Chains Antibody [DyLight 488]NBP1-72944RbFLISA, IF, WB(3)

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