Beta Actin Antibody (NB600-503)
Beta Actin Antibody
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Distributor Data...
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anti-Actin beta antibody, Anti-Beta actin antibody, Anti-Beta-actin antibody, anti-ACTB antibody, Anti-A X actin like protein antibody, Anti-PS1TP5BP1 antibody, Anti-Melanoma X actin antibody, antibody, Anti-Beta-actin antibody, Anti-Beta cytoskeletal actin, anti-beta antibody, Anti-Beta actin antibody, anti-b-actin antibody, Anti-Actx antibody, anti-Actin cytoplasmic 1 antibody, anti-Actin antibody, |
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Beta Actin Antibody Summary: | | Gene: | ACTB |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | Specificity: | Beta actin |
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| Publications: | Antibody has been mentioned in at least 44 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Beta Actin Products Beta Actin Antibody Details: | Immunogen: | Synthetic peptide derived from the N-terminus of human Beta Actin. |
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| Localization: | Cytoplasmic |
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Cross-reacts with Human, Rat, Monkey and Mouse. Expected to cross-react with a wide range of species due to sequence homology. Not yet tested in other species. |
| Uses: | This beta actin antibody is useful for Western blotting, Immunoprecipitation, Immunofluorescence and Immunocytochemistry |
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| Dilutions: | Immunocytochemistry 1:50-1:200, Immunofluorescence 1:50-1:200, immunoprecipitation, Western Blot |
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| Unit Size: | 0.05 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 1.0 mg/ml |
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| Positive Controls: | 3 Positive Controls |
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| Conjugated Antibodies: | 5 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | Tris-citrate/phosphate buffer, pH 7 |
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| Preservative: | 0.09% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta actin and the static and dynamic aspects of the cytoskeleton.
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Images (2) Reviews (1) Related Products (57) Exploring Beta Actin Working with Beta Actin
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Jump to: Publications, Images, Reviews, Ask a Question
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Working with Beta Actin
Beta Actin Antibody Images (2) Western Blot: Beta Actin Antibody [NB600-503] - Rabbit polyclonal at 1/5000. |  Immunocytochemistry: Beta Actin Antibody [NB600-503] - Staining in HeLa cell |
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Reviewed by:
Anonymous 11/18/2010
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Application:
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Western Blot
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Sample Tested:
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NIH 3T3 cell lysate
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Sample Loaded:
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5 µl
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Species:
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Mouse
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Sample Pretreated:
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RIPA buffer
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Blocking Buffer:
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5% Milk in PBST
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Concentration:
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5%
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Blocking Time:
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1 h
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Blocking Temperature:
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20? Celsius
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Dilution Ratio:
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1:5000
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Incubation Time:
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O/N
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Incubation Temperature:
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4? Celsius
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Incubation Dilution Buffer:
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5% Milk in PBST
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Description:
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anti-mouse IgG HRP labeled
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Manufacturer Catalog #:
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NA9310
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Dilution Ratio:
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1:5000
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Method:
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ECL
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Exposure Time:
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30 sec
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Positive Control:
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-
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Negative Control:
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-
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Did you observe bands?:
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Yes
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Specific Bands:
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around 40 kDa kDa
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Non-specific Bands:
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- kDa
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Publications (44)Beta Actin Antibody Product Specific:- Kumamoto CA. A contact-activated kinase signals Candida albicans invasive growth and biofilm development. Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5576-81. [PMID: 15800048] (Loading Control)
- Frei E, Huster M, Smital P, et al. Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle. Am J Physiol Gastrointest Liver Physiol 2009;297(4):G834-839. [PMID: 19628652]
more... - Babic I, Sharma S, Black DL. A Role for Polypyrimidine Tract Binding Protein in the Establishment of Focal Adhesions. Mol Cell Biol 2009;29(20):5564-5577. [PMID: 19667078]
- Pillemer BBL, Qi Z, Melgert B, et al. STAT6 Activation Confers upon T Helper Cells Resistance to Suppression by Regulatory T Cells. J Immunol 2009;183(1):155-163. [pubmed: 19535633]
- Sane, A.T., et al. Localization and role of NPC1L1 in cholesterol absorption in human intestine. J Lipid Res. 2006 Oct;47(10):2112-20. Epub 2006 Jul 7.
- Younis, I., et al. Human T-cell leukemia virus open reading frame II encodes a posttranscriptional repressor that is recruited at the level of transcription. J Virol. 2006 Jan;80(1):181-91.
- Sirinyan, M., et al. Hyperoxic exposure leads to nitrative stress and ensuing microvascular degeneration and diminished brain mass and function in the immature subject. Stroke. 2006 Nov;37(11):2807-15. Epub 2006 Sep 28.
- Ostroukhova, M., et al. Treg-mediated immunosuppression involves activation of the Notch-HES1 axis by membrane-bound TGF-beta. J Clin Invest. 2006 Apr;116(4):996-1004. Epub 2006 Mar 16.
- Oganesian, A., et al. The NH2-terminal propeptide of type I procollagen acts intracellularly to modulate cell function. J Biol Chem. 2006 Dec 15;281(50):38507-18. Epub 2006 Oct 3.
- Nadauld, L. D., et al. Adenomatous polyposis coli control of C-terminal binding protein-1 stability regulates expression of intestinal retinol dehydrogenases. J Biol Chem. 2006 Dec 8;281(49):37828-35. Epub 2006 Oct 6.
- Koivunen, P., et al. Inhibition of hypoxia-inducible factor (HIF) hydroxylases by citric acid cycle intermediates: possible links between cell metabolism and stabilization of HIF. J Biol Chem. 2007 Feb 16;282(7):4524-32. Epub 2006 Dec 19.
- Jovic, M., et al. EHD1 regulates beta1 integrin endosomal transport: effects on focal adhesions, cell spreading and migration. J Cell Sci. 2007 Mar 1;120(Pt 5):802-14. Epub 2007 Feb 6.
- Johnson, J. D., et al. Insulin protects islets from apoptosis via Pdx1 and specific changes in the human islet proteome. Proc Natl Acad Sci U S A. 2006 Dec 19;103(51):19575-80. Epub 2006 Dec 8.
- Dennis, V. A., et al. Interleukin-10 anti-inflammatory response to Borrelia burgdorferi, the agent of Lyme disease: a possible role for suppressors of cytokine signaling 1 and 3. Infect Immun. 2006 Oct;74(10):5780-9.
- Csiszar, A., et al. Bone morphogenetic protein-2 induces proinflammatory endothelial phenotype. Am J Pathol. 2006 Feb;168(2):629-38.
- Carlson, D., et al. Antioxidant vitamin therapy alters sepsis-related apoptotic myocardial activity and inflammatory responses. Am J Physiol Heart Circ Physiol. 2006 Dec;291(6):H2779-89. Epub 2006 Jul 14.
- Cambonie, G., et al. Antenatal antioxidant prevents adult hypertension, vascular dysfunction, and microvascular rarefaction associated with in utero exposure to a low-protein diet. Am J Physiol Regul Integr Comp Physiol. 2007 Mar;292(3):R1236-45. Epub 2006 Nov 30.
- Brault, S., et al. Lysophosphatidic acid induces endothelial cell death by modulating the redox environment. Am J Physiol Regul Integr Comp Physiol. 2007 Mar;292(3):R1174-83. Epub 2006 Nov 22.
- Boutz, P. L., Chawla, G., Stoilov, P., Black, D. L. MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development. Genes Dev. 2007 Jan 1;21(1):71-84.
- Yang C, Lafleur J, Mwaikambo BR, et al. The Role of Lysophosphatidic Acid Receptor (LPA1) in the Oxygen-Induced Retinal Ganglion Cell Degeneration. Invest Ophthalmol Vis Sci 2009;50(3):1290-1298 [pubmed: 18978343]
- Liu J, Zhang J-P, Shi M, et al. Rab11a HSP90 Regulate Recycling of Extracellular {alpha}-Synuclein. J Neurosci. 2009 Feb 4;29(5):1480-5. [pubmed: 19193894]
- Staquicini FI, Dias-Neto E, Li J, et al. Discovery of a functional protein complex of netrin-4, laminin {gamma}1 chain, integrin {alpha}6{beta}1 in mouse neural stem cells. Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2903-8. [pubmed: 19193855]
- Kaczmarek M, Cachau RE, Topol IA, et al. Metal Ions-Stimulated Iron Oxidation in Hydroxylases Facilitates Stabilization of HIF-1{alpha} Protein. Toxicol Sci. 2009 Feb;107(2):394-403. [pubmed: 19074761]
- Ghosh AK, Kay NE, Secreto CR, et al. Curcumin Inhibits Prosurvival Pathways in Chronic Lymphocytic Leukemia B Cells May Overcome Their Stromal Protection in Combination with EGCG. Clin Cancer Res. 2009 Feb 15;15(4):1250-8. [pubmed: 19228728]
- Stice JP, Eiserich JP, Knowlton AA. Role of aging versus the loss of estrogens in the reduction in vascular function in female rats. Endocrinology. 2009 Jan;150(1):212-9. Epub 2008 Sep 11.
- Ueno, H., Kejihara, H., Nakamura, H., et al. Contribution of thioredoxin reductase to T-cell mitogenesis and NF-kappaB DNA-binding promoted by selenite. Antioxid Redox Signal. 2007 Jan;9(1):115-21.
- Ougolkov AV, Bilim VN, Billadeau DD. Regulation of pancreatic tumor cell proliferation and chemoresistance by the histone methyltransferase enhancer of zeste homologue 2. Clin Cancer Res. 2008 Nov 1;14(21):6790-6.
- Elorza A, Hyde B, Mikkola HK, et al. UCP2 modulates cell proliferation through the MAPK/ERK pathway during erythropoiesis and has no effect on heme biosynthesis. J Biol Chem. 2008 Nov 7;283(45):30461-70. Epub 2008 Aug 7.
- Young JNS, Guttman JA, Vaid KS, et al. Tubulobulbar complexes are intercellular podosome-like structures that internalize intact intercellular junctions during epithelial remodeling events in the rat testis. Biol Reprod. 2009 Jan;80(1):162-74. Epub 2008 Sep 17.
- Martinsson-Ahlzen H-S, et al. Cyclin-dependent kinase-associated proteins Cks1 and Cks2 are essential during early embryogenesis and for cell cycle progression in somatic cells. Mol Cell Biol. 2008 Sep;28(18):5698-709. Epub 2008 Jul 14.
- Park, K., et al. Conditional neuronal simian virus 40 T antigen expression induces Alzheimer-like tau and amyloid pathology in mice. J Neurosci. 2007 Mar 14;27(11):2969-78.
- He, X., et al. Apolipoprotein receptor 2 and X11 alpha/beta mediate apolipoprotein E-induced endocytosis of amyloid-beta precursor protein and beta-secretase, leading to amyloid-beta production. J Neurosci. 2007 Apr 11;27(15):4052-60.
- Zou, D., et al. Absence of adenylyl cyclase 3 perturbs peripheral olfactory projections in mice. J Neurosci. 2007 Jun 20;27(25):6675-83.
- Garg, A., et al. Vimentin expressed on Mycobacterium tuberculosis-infected human monocytes is involved in binding to the NKp46 receptor. J Immunol. 2006 Nov 1;177(9):6192-8. Erratum in: J Immunol. 2008 Dec 15;181(12):8797.
- Gobeil, F., Jr., Zhu, T., Brault, S., et al. (2006) Nitric oxide signaling via nuclearized endothelial nitric-oxide synthase modulates expression of the immediate early genes iNOS and mPGES-1. J Biol Chem. 2006 Jun 9;281(23):16058-67. Epub 2006 Mar 30.
- Wang, W., et al. PC cell-derived growth factor confers resistance to dexamethasone and promotes tumorigenesis in human multiple myeloma. Clin Cancer Res. 2006 Jan 1;12(1):49-56.
- He, X., et al. GGA proteins mediate the recycling pathway of memapsin 2 (BACE). J Biol Chem. 2005 Mar 25;280(12):11696-703. Epub 2004 Dec 21.
- Watters, J., et al. Genome-wide discovery of loci influencing chemotherapy cytotoxicity. Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11809-14. Epub 2004 Jul 28.
- Mukherjee, P., et al. Antiangiogenic and proapoptotic effects of dietary restriction on experimental mouse and human brain tumors. Clin Cancer Res. 2004 Aug 15;10(16):5622-9.
- Adler, S., et al. Oxidant stress leads to impaired regulation of renal cortical oxygen consumption by nitric oxide in the aging kidney. J Am Soc Nephrol. 2004 Jan;15(1):52-60.
- Adler, S., et al. Oxidant stress in kidneys of spontaneously hypertensive rats involves both oxidase overexpression and loss of extracellular superoxide dismutase. Am J Physiol Renal Physiol. 2004 Nov;287(5):F907-13.
- Sun, D., et al. Reduced release of nitric oxide to shear stress in mesenteric arteries of aged rats. Am J Physiol Heart Circ Physiol. 2004 Jun;286(6):H2249-56. Epub 2004 Jan 29.
- Sung, S., et al. Modulation of nuclear factor-kappa B activity by indomethacin influences A beta levels but not A beta precursor protein metabolism in a model of Alzheimer's disease. Am J Pathol. 2004 Dec;165(6):2197-206.
- Witting, S. et al. Ceramide enhances cholesterol efflux to apolipoprotein A-I by increasing the cell surface presence of ATP-binding cassette transporter A1. J Biol Chem. 2003 Oct 10;278(41):40121-7. Epub 2003 Jul 30.
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Jump to: Conjugated Antibodies, Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Beta Actin Antibody [DyLight 488] | NB600-503G | Hu, Mk, Mu, Rt | IF, IP, WB | | (42) | | | | Beta Actin Antibody [HRP] | NB600-503H | Hu, Mk, Mu, Rt | IF, IP, WB | | (42) | | | | Beta Actin Antibody [DyLight 550] | NB600-503R | Hu, Mk, Mu, Rt | IF, IP, WB | | (42) | | | | Beta Actin Antibody [Biotin] | NB600-503B | Hu, Mk, Mu, Rt | IF, IP, WB | | (42) | | | | Beta Actin Antibody [DyLight 650] | NB600-503C | Hu, Mk, Mu, Rt | IF, IP, WB | | (42) | | |
Positive ControlsLysates (2)Peptides and ProteinsPrimary Antibodies (43)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Beta Actin Antibody (AC-15) | NB600-501 | Bv, Ca, Ch, Dr(-), Fe, Fi, GP, Hu, Le, Mu, Po, Rb, Rt, Sh | ELISA, ICC, IF, IHC-Fr, WB | (5) | (37) | | | | Beta Actin Antibody (AC-15) | NB110-67828 | Bv, Ca, Ch, Fe, Fi, GP, Hu, Mu, Po, Rb, Rt, Sh | ICC, IF, IHC-Fr, WB | (1) | (26) | | | | Beta Actin Antibody | NB600-532 | Hu, Mu | WB | (3) | (8) | | | | Beta Actin Antibody (Poly6221) | NB100-78420 | Hu, Mu, Rt | IF, IHC-P, WB | (3) | (7) | | | | Beta Actin Antibody (3G4-F9) | H00000060-M01 | Hu, Mu | ELISA, IHC-P, WB | (7) | (2) | | | | ... and 38 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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