Beta Actin Antibody (NB600-501)
Beta Actin Antibody (AC-15)
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Anti-Beta-actin antibody, anti-ACTB antibody, Anti-A X actin like protein antibody, beta antibody, Anti-PS1TP5BP1 antibody, Anti-Melanoma X actin antibody, Anti-Beta-actin antibody, Anti-Beta cytoskeletal actin antibody, Anti-Beta actin antibody, anti-b-actin antibody, Anti-Actx antibody, Anti-Actin, |
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Beta Actin Antibody (AC-15) Summary: | Species: | Bv, Ca, Ch, Dr(-), Fe, Fi, GP, Hu, Le, Mu, Po, Rb, Rt, Sh |
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| Applications: | ELISA, ICC, IF, IHC-Fr, WB |
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| Clonality: | Monoclonal |
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| | Gene: | ACTB |
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| Purity: | Ascites |
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| Host: | Mouse |
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| | Specificity: | In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin's solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding. |
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| Publications: | Antibody has been mentioned in at least 37 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Beta Actin Products Beta Actin Antibody (AC-15) Details: | Clone: | AC-15 |
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| Isotype: | IgG1 |
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| Immunogen: | slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu -Val-Ile-Asp-Asn-Gly-Ser-Gly-L ys, conjugated to KLH. |
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| Epitope: | N-terminus of the beta isoform of actin. |
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The antibody cross reacts with b-actin expressing cells in bovine, canine, chicken, feline, carp, leech tissues, guinea pig, human, mouse, porcine, rabbit, rat, ground squirrel and sheep.
Does not cross react with adult cardiac, skeletal muscle, drosophila or amoeba beta actin. Not yet tested in other species. |
| Uses: | This antibody is useful for Western blot, ELISA, immunohistochemistry and Immunocytochemistry. Molecular weight of beta actin is 42kD. The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. This antibody is not suitable for formalin fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives. |
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| Dilutions: | ELISA, Immunocytochemistry 1:1000, Immunofluorescence 1:1000-1:2000, Immunohistochemistry-Frozen 1:500, Western Blot 1:5000-1:10000 |
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| Unit Size: | 0.1 ml (also available: 0.05 ml (sample size)) |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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| Positive Controls: | 1 Positive Controls |
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| Conjugated Antibodies: | 4 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Preservative: | 15mM Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
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Images (5) Reviews (12) Related Products (57) Exploring Beta Actin Working with Beta Actin
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with Beta Actin
Beta Actin Antibody (AC-15) Images (5) Micrograph Image:Beta-actin Antibody (AC-15) [NB600-501] -Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double labeled with Monoclonal Anti-alpha-Actin and polyclonal antibodies to chicken gizzard myosin (red). d-f. Longitudinal, expanded cryosections of chicken gizzard muscle double labeled with polyclonal Anti-alpha-Actinin (green, d, e; red, f) in combination with antibodies to chicken gizzard myosin, antibodies to chicken desmin and Monoclonal Anti-â-Actin,(f, green). Bars, 10 mm. From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg. |  Western blot: Beta-actin Antibody (AC-15) [NB600-501] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-alpha-Actin The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lanes A: Antibody dilution 1:5,000 B: Negative control (only secondary antibody) |  WB: Beta-actin Antibody [NB600-501] -MCDK cells induced with doxycycline to control the expression of the gene of interest. Beta actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane. |  Immunofluorescence: Beta-actin Antibody (AC-15) [NB600-501] - Immunofluroescence Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-â Actin, Clone No. AC-15 (NB600-501). |  Immunohistochemistry: Beta-actin Antibody (AC-15) [NB600-501] -Breast, Epithelium 40x |
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Reviewed by:
Che-Yi Chao 12/21/2011
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Application:
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Western Blot
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Sample Tested:
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Mouse BALB/c macrophage cell line
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Species:
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Mouse
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Blocking Buffer:
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5% non-fat milk in PBST
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Blocking Time:
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1 hr.
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Dilution Ratio:
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1:1000
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Incubation Time:
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24 hrs.
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Incubation Temperature:
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4 Celsius
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Description:
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Goat anti-Mouse IgG (HRP), Jackson
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Method:
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GE ImageQuant LAS 4000 mini plus ECL
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Additional Comments:
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The cultured cells were harvested, washed and lysed. Extracts were stored at -20C prior to analysis
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Reviewed by:
Ssu-Ching Wang 12/14/2011
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Application:
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Western Blot
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Sample Tested:
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Mouse
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Species:
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Mouse
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Sample Pretreated:
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Add sample buffer and heat at 90 C for 3 min
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Dilution Ratio:
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1:5000
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Method:
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HRP System, detect ECL signal by photographic film
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Reviewed by:
Shun-Fa Yang 12/5/2011
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Application:
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Western Blot
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Sample Tested:
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Human
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Species:
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Human
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Sample Pretreated:
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SDS and ?-mercaptoethanol. Heat 100c for 10 min
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Results:
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Good!
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Blocking Buffer:
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3% BSA for 2 h
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Dilution Ratio:
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1:5000
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Incubation Time:
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16h
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Incubation Temperature:
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4 Celsius
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Description:
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Mouse Millipore
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Dilution Ratio:
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1:10000
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Reviewed by:
Ping-Sung Chiu 11/30/2011
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Application:
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Western Blot
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Sample Tested:
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Mouse Brain
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Species:
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Mouse
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Blocking Buffer:
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5% non-fat dry milk in PBS
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Dilution Ratio:
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1:5000
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Description:
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Anti-Mouse IgG HRP
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Dilution Ratio:
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1:10000
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Reviewed by:
Chien-Hui Yu 11/21/2011
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Application:
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Western Blot
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Sample Tested:
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Human cell lines
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Species:
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Human
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Sample Pretreated:
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Purified protein
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Dilution Ratio:
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1:5000
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Incubation Time:
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overnight
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Reviewed by:
Hsien Sheng Tin 11/9/2011
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Application:
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Western Blot
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Sample Tested:
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Chicken cell lines
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Species:
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Chicken
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Sample Pretreated:
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None
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Dilution Ratio:
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1:1000
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Incubation Time:
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1 h
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Incubation Temperature:
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RT
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Description:
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Anti-mouse HRP
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Reviewed by:
Anonymous 9/2/2011
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Application:
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Western Blot
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Sample Tested:
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Mouse Cells
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Species:
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Mouse
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Blocking Buffer:
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HyBlock blocking buffer from HyCell Biotec
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Blocking Time:
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1 minute
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Dilution Ratio:
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1:5000
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Incubation Time:
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10 minutes
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Method:
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Viaglo HRP Chemilum Substrate Kit
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Negative Control:
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Beta Actin
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Reviewed by:
Anonymous 8/11/2011
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Application:
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Western Blot
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Sample Tested:
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Ground squirrel small intestinal lysate
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Sample Loaded:
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50 ug
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Species:
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Other
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Species Other:
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13-lined ground squirrel
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Results:
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Antibody worked very well, recognized unusual species (ground squirrel) at 1:5000 dilution with colorimetric detection!
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Blocking Buffer:
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4% milk
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Blocking Time:
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1 hr
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Blocking Temperature:
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RT
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Dilution Ratio:
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1:5000
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Incubation Time:
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Overnight
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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4% milk
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Description:
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Goat anti-mouse poly-HRP
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Dilution Ratio:
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1:1500
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Method:
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Opti-4CN
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Exposure Time:
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< 10 s
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Did you observe bands?:
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Yes
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Specific Bands:
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~42 kDa
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Reviewed by:
Anonymous 8/30/2010
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Application:
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Western Blot
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Sample Tested:
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293T whole cell lysate, adult neural stem cell whole cell lysate
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Species:
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Mouse
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Results:
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Very strong detection with 1:5000 dilution
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Reviewed by:
Zi-Jun Lin 12/21/2011
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Application:
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Western Blot
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Sample Tested:
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Human cancer cell line
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Species:
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Human
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Sample Pretreated:
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heat at 100C for 5 min
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Dilution Ratio:
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1:5000
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Incubation Time:
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overnight
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Incubation Temperature:
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4 Celsius
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Reviewed by:
Hui-Chen Lin 12/8/2011
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Application:
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Western Blot
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Sample Tested:
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Human
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Species:
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Human
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Blocking Buffer:
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5% non-fat milk
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Dilution Ratio:
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1:10000
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Incubation Time:
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1 h
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Incubation Temperature:
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RT
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Description:
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anti-mouse IgG HRP
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Reviewed by:
Chiung-Chi Peng 12/6/2011
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Application:
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Western Blot
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Sample Tested:
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Rat
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Species:
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Rat
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Sample Pretreated:
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Doxorubicin
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Dilution Ratio:
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1:5000
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Incubation Time:
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1 h
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Publications (37)Beta Actin Antibody (AC-15) Product Specific:- Ge X, Yin L, Ma H, et al. Aldo-keto reductase 1B7 is a target gene of FXR and regulates lipid and glucose homeostasis.Epub 2011 Jun 5.[PMID 21642744]
- Raats DAE, de Bruijn MT, Steller EJA, et al. Synergistic killing of colorectal cancer cells by oxaliplatin and ABT-737. Cell Oncol (Dordr). 2011 Aug;34(4):307-13.[PMID 21468686]
more... - L Yang, Z Yang, R Guo, et al. Angiotensin II induces extracellular matrix metalloproteinase inducer expression via an AT1R dependent pathway in aortic atherosclerotic plaque in apolipoprotein E knockout mice.J Renin Angiotensin Aldosterone Syst. 2011 Oct 21.[PMID:22020146]
- Scarisbrick IA, Epstein B, Cloud BA, et al. Functional Role of Kallikrein 6 in Regulating Immune Cell Survival. PLoS ONE. 2011 Mar 28.[PMID 21464892]
- Huang AM, Kao YT, Toh S, et al. UBE2M-mediated p27(Kip1) degradation in gemcitabine cytotoxicity.Biochem Pharmacol. 2011 Apr 5. [PMID: 21477582]
- Hildebrand JM, Luo Z, Manske MK, et al. A BAFF-R mutation associated with non-Hodgkin lymphoma alters TRAF recruitment and reveals new insights into BAFF-R signaling. J Exp Med. 2010 Nov 1. [PMID: 21041452]
- Macchi B, Minutolo A, Grelli S, et al. The novel proapoptotic activity of nonnatural enantiomer of Lentiginosine.Glycobiology. 2010 May;20(5):500-6 [PMID: 20053629(Human, WB)
- M. Maj, W. Gartner, A. Ilhan, et al. Expression of TAU in insulin-secreting cells and its interaction with the calcium-binding protein secretagogin. J. Endocrinol. 2010; 205 (1): 25-36. [PMID: 20061514]
- Liao, J., et al. Direct reprobing with anti-beta-actin antibody as an internal control for western blotting analysis. Biotechniques. 2000 Feb;28(2):216-8.
- North, A.J., et al. Calponin is localised in both the contractile apparatus and the cytoskeleton of smooth muscle cells. J Cell Sci. 1994 Mar;107 ( Pt 3):437-44.
- Gimona, M., et al. Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 1994;27(2):108-16.
- North, A.J., et al. Actin isoform compartments in chicken gizzard smooth muscle cells. J Cell Sci. 1994 Mar;107 ( Pt 3):445-55.
- Barthel SR, Gavino JD, Wiese GK, et al. Analysis of glycosyltransferase expression in metastatic prostate cancer cells capable of rolling activity on microvascular endothelial (E)-selectin. Glycobiology. 2008 Oct;18(10):806-17. Epub 2008 Jul 22.
- Dror V, Kalynyak TB, Bychkivska Y, et al. Glucose and endoplasmic reticulum calcium channels regulate HIF-1beta via presenilin in pancreatic beta-cells. J Biol Chem. 2008 Apr 11;283(15):9909-16. Epub 2008 Jan 3.
- Heazlewood, C., et al. Aberrant mucin assembly in mice causes endoplasmic reticulum stress and spontaneous inflammation resembling ulcerative colitis. PLoS Med. 2008 Mar 4;5(3):e54.
- Alejandro EU, Johnson JD. Inhibition of Raf-1 alters multiple downstream pathways to induce pancreatic beta-cell apoptosis. J Biol Chem. 2008 Jan 25;283(4):2407-17. Epub 2007 Nov 15.
- Zhang, S., et al. OSU-03012, a novel celecoxib derivative, is cytotoxic to myeloma cells and acts through multiple mechanisms. Clin Cancer Res. 2007 Aug 15;13 (16):4750-8.
- Ougolkov, A., et al. Inhibition of glycogen synthase kinase-3 activity leads to epigenetic silencing of nuclear factor kappaB target genes and induction of apoptosis in chronic lymphocytic leukemia B cells. Blood. 2007 Jul 15;110(2):735-42. Epub 2007 Apr 26.
- Park, K., et al. Conditional neuronal simian virus 40 T antigen expression induces Alzheimer-like tau and amyloid pathology in mice. J Neurosci. 2007 Mar 14;27(11):2969-78.
- Greer, E.L. et al. The energy sensor AMP-activated protein kinase directly regulates the mammalian FOXO3 transcription factor. J Biol Chem. 2007 Oct 12;282(41):30107-19. Epub 2007 Aug 20.
- Abramovich D, Parborell F, Tesone M. Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats. Biol Reprod. 2006 Sep;75(3):434-41. Epub 2006 Jun 14.
- Dennis, Vida A., et al. Interleukin-10 anti-inflammatory response to Borrelia burgdorferi, the agent of Lyme disease: a possible role for suppressors of cytokine signaling 1 and 3. Infect Immun. 2006 Oct;74(10):5780-9
- Csiszar, A., et al. Bone morphogenetic protein-2 induces proinflammatory endothelial phenotype. Am J Pathol. 2006 Feb;168(2):629-38.
- Moiseyev, G., et al. RPE65 is the isomerohydrolase in the retinoid visual cycle. Proc Natl Acad Sci U S A. 2005 Aug 30;102(35):12413-8. Epub 2005 Aug 22.
- Liu, X., Manser, T. Antinuclear antigen B cells that down-regulate surface B cell receptor during development to mature, follicular phenotype do not display features of anergy in vitro. J Immunol. 2005 Apr 15;174(8):4505-15.
- Rice, P.L., et al. Rice PL, Peters SL, Beard KS, Ahnen DJ. Sulindac independently modulates extracellular signal-regulated kinase 1/2 and cyclic GMP-dependent protein kinase signaling pathways. Mol Cancer Ther. 2006 Mar;5(3):746-54.
- Dietz, A., et al. Imatinib mesylate inhibits T-cell proliferation in vitro and delayed-type hypersensitivity in vivo. Blood. 2004 Aug 15;104(4):1094-9. Epub 2004 Apr 20.
- Watters, J., et al. Genome-wide discovery of loci influencing chemotherapy cytotoxicity. Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11809-14. Epub 2004 Jul 28.
- Laughlin, R., et al. Involvement of raft-like plasma membrane domains of Entamoeba histolytica in pinocytosis and adhesion. Infect Immun. 2004 Sep;72(9):5349-57.
- Mukherjee, P., et al. Antiangiogenic and proapoptotic effects of dietary restriction on experimental mouse and human brain tumors. Clin Cancer Res. 2004 Aug 15;10(16):5622-9.
- Adler, S., et al. Oxidant stress leads to impaired regulation of renal cortical oxygen consumption by nitric oxide in the aging kidney. J Am Soc Nephrol. 2004 Jan;15(1):52-60.
- Adler S, Huang H. Oxidant stress in kidneys of spontaneously hypertensive rats involves both oxidase overexpression and loss of extracellular superoxide dismutase. Am J Physiol Renal Physiol. 2004 Nov;287(5):F907-13.
- Sun, D., et al. Reduced release of nitric oxide to shear stress in mesenteric arteries of aged rats. Am J Physiol Heart Circ Physiol. 2004 Jun;286(6):H2249-56. Epub 2004 Jan 29.
- Sung, S., et al. Modulation of nuclear factor-kappa B activity by indomethacin influences A beta levels but not A beta precursor protein metabolism in a model of Alzheimer's disease. Am J Pathol. 2004 Dec;165(6):2197-206.
- Chong, V., et al. Differential display polymerase chain reaction reveals increased expression of striatal rat glia-derived nexin following chronic clozapine treatment. Pharmacogenomics J. 2004;4(6):379-87.
- Thornton, M., et al. Degradation of NF-kappa B in T cells by gangliosides expressed on renal cell carcinomas. J Immunol. 2004 Mar 15;172(6):3480-90. (Western blot, human)
- Hawkins BS, Fischer LJ. Inhibition of insulin synthesis by cyproheptadine: effects on translation. Toxicol Sci. 2004 Jun;79(2):258-65. Epub 2004 Mar 31.
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Jump to: Conjugated Antibodies, Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Beta Actin Antibody (AC-15) [DyLight 488] | NB600-501G | Bv, Ca, Ch, Dr, Fe, Fi, GP, Hu, Mu, Po, Rb, Rt, Sh | ELISA, ICC, IF, IHC, WB | | (32) | | | | Beta Actin Antibody (AC-15) [DyLight 550] | NB600-501R | Bv, Ca, Ch, Dr, Fe, Fi, GP, Hu, Mu, Po, Rb, Rt, Sh | ELISA, ICC, IF, IHC, WB | | (32) | | | | Beta Actin Antibody (AC-15) [DyLight 650] | NB600-501C | Bv, Ca, Ch, Dr, Fe, Fi, GP, Hu, Mu, Po, Rb, Rt, Sh | ELISA, ICC, IF, IHC, WB | | (32) | | | | Beta Actin Antibody (AC-15) [HRP] | NB600-501H | Bv, Ca, Ch, Dr, Fe, Fi, GP, Hu, Mu, Po, Rb, Rt, Sh | ELISA, ICC, IF, IHC, WB | | (31) | | |
Positive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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Other Positive Controls- Cultured human or chicken fibroblasts, as described in Liao et al.
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Lysates (2)Peptides and ProteinsPrimary Antibodies (43)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Beta Actin Antibody | NB600-503 | Hu, Mk, Mu, Rt | ICC, IF, IP, WB | (2) | (44) | | | | Beta Actin Antibody (AC-15) | NB110-67828 | Bv, Ca, Ch, Dr(-), Fe, Fi, GP, Hu, Mu, Po, Rb, Rt, Sh | ELISA, ICC, IF, IHC-Fr, WB | (1) | (26) | | | | Beta Actin Antibody | NB600-532 | Hu, Mu | WB | (3) | (8) | | | | Beta Actin Antibody (Poly6221) | NB100-78420 | Hu, Mu, Rt | IF, IHC-P, WB | (3) | (7) | | | | Beta Actin Antibody (3G4-F9) | H00000060-M01 | Hu, Mu | ELISA, IHC-P, WB | (7) | (2) | | | | ... and 38 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Mouse IgG Antibody [Biotin] | NB720-B | Mu | ELISA, IHC-P, WB | | | | | | Mouse IgG Antibody [HRP] | NB720-H | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [Alkaline Phosphatase] | NB720-AP | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [FITC] | NB720-F | Mu | ICC, IHC-P | | (2) | | | | Mouse IgG Antibody | NB120-7056 | Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-) | ELISA, IHC-P, IP, WB | | | | | | See All » |
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