Bestrophin 1 Antibody (NB300-164)
Bestrophin 1 Antibody (E6-6)
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anti-VMD2L1 antibody, anti-Vitelliform macular dystrophy protein 2 antibody, anti-VMD 2 antibody, anti-Vitelliform macular dystrophy antibody, anti-Vitelliform macular dystrophy 2 antibody, anti-mBest1 antibody, anti-Bestrophin1 antibody, anti-Bestrophin 1 antibody, anti-BEST1 antibody, anti-Best macular dystrophy antibody, anti-BEST 1 antibody, anti-VMD2 antibody, anti-TU15B antibody, anti-FLJ20132 antibody, anti-BMD antibody, anti-BEST antibody |
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Bestrophin 1 Antibody (E6-6) Summary: | | Gene: | BEST1 |
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| Purity: | Ascites |
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| Host: | Mouse |
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| | Specificity: | Bestrophin (E6-6) |
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| Publications: | Antibody has been mentioned in at least 7 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Bestrophin 1 Products Bestrophin 1 Antibody (E6-6) Details: | Clone: | E6-6 |
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| Isotype: | IgG1 Kappa |
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| Immunogen: | Synthetic peptide conjugated to KLH [KDHMDPYWALENRDEAHS].
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NB 300-164 recognizes human, porcine, and monkey. This antibody does not work in rat. Other species have not been tested. |
| Uses: | By Western blot, this antibody recognizes a band at ~68 kDa representing Bestrophin.** It is also useful for immunofluorescence. **Please see protocol for treatment of cell extract. |
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| Dilutions: | immunofluorescence Assay dependent,
Western Blot 1:1,000 |
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| TMW: | 68 kDa |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
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| Preservative: | 0.1% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Best macular dystrophy (BMD) or vitelliform macular dystrophy (VMD2), is an autosomal form of macular degeneration, characterized by a depressed light peak in the electrooculogram (EOG). It is inherited and has an early onset. Bestrophin is a 68 kDa basolateral plasma membrane protein encoded by the VMD2 gene. Bestrophin's function is still unknown, but data suggests that it is a chloride channel that plays a role in generating the altered EOG in Best disease patients. In addition, Bestrophin is a useful biochemical and histological marker of RPE (retinal pigment epithelial cells) cells.
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Images (1) Reviews (0) Related Products (8) Exploring Bestrophin 1 Working with Bestrophin 1
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Jump to: Publications, Images, Protocols/Procedures, Ask a Question
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Working with Bestrophin 1
Bestrophin 1 Antibody (E6-6) Images (1) Western Blot: Bestrophin Antibody (E6-6) [NB300-164] - Western blot detection of Bestrophin (68 kDa) from human RPE cell lysate. |
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Publications (7)Bestrophin 1 Antibody (E6-6) Product Specific:- Guziewicz KE, Zangerl B, Lindauer SJ, Mullins RF, Sandmeyer LS, Grahn BH, Stone EM, Acland GM, Aguirre GD. Bestrophin gene mutations cause canine multifocal retinopathy: a novel animal model for best disease. Invest Ophthalmol Vis Sci. 2007 May;48(5):1959-67. PubMed PMID: 17460247. [WB, IF, Canine]
- Marmorstein LY, McLaughlin PJ, Stanton JB, Yan L, Crabb JW, Marmorstein AD. Bestrophin interacts physically and functionally with protein phosphatase 2A. J Biol Chem. 2002 Aug 23;277(34):30591-7. Epub 2002 Jun 10. PubMed PMID: 12058047.
more... - Marmorstein AD, Marmorstein LY, Rayborn M, Wang X, Hollyfield JG, Petrukhin K. Bestrophin, the product of the Best vitelliform macular dystrophy gene (VMD2), localizes to the basolateral plasma membrane of the retinal pigment epithelium. Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12758-63. PubMed PMID: 11050159. [WB, IP, IHC-F, IF, h, macaque, and porcine]
- Harness JV, Turovets NA, Seiler MJ, et al. Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells. PLoS ONE. 2011;6(1).[PMID: 21249129]
- Guziewicz KE, Slavik J, Lindauer SJ, et al. Molecular consequences of BEST1 gene mutations in canine multifocal retinopathy predict functional implications for human bestrophinopathies. Invest Ophthalmol Vis Sci. 2011 Apr 15. [PMID:21498618]
- Zhang Y, Patil RV, Marmorstein AD. Bestrophin 2 is expressed in human non-pigmented ciliary epithelium but not retinal pigment epithelium. Mol Vis. 2010 Feb 10;16:200-6.
- Klimanskaya, I., Hipp, J., Rezai, K., et al. Derivation and comparative assessment of retinal pigment epithelium from human embryonic stem cells using transcriptomics. Cloning Stem Cells. 2004;6(3):217-45.
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Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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Other Positive Controls- Pig RPE (Retinal Pigment Epithelium) whole cell extract
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Lysates (1)Peptides and ProteinsPrimary Antibodies (3)RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Mouse IgG Antibody [Biotin] | NB720-B | Mu | ELISA, IHC-P, WB | | | | | | Mouse IgG Antibody [HRP] | NB720-H | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [Alkaline Phosphatase] | NB720-AP | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [FITC] | NB720-F | Mu | ICC, IHC-P | | (2) | | | | Mouse IgG Antibody | NB120-7056 | Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-) | ELISA, IHC-P, IP, WB | | | | | | See All » |
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