Beclin 1 Antibody [DyLight 649]

Species: Mu, Hu Applications: IHC-P, WB, IP, IF, IHC Clonality: Polyclonal	Gene: BECN1 Purity: Immunogen affinity purified Host: Rabbit
Specificity: This is specific to human Beclin 1. Publications: Antibody has been mentioned in at least 7 publications.
See All Beclin 1 Products See All Beclin 1 Related Products: Antibody Packs Lysates Peptides and Proteins Primary Antibodies
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Conjugate:	DyLight 649
Immunogen:	NB 500-249 was raised against an internal synthetic peptide to human Beclin 1, containing residues within 1-100. This sequence has 94% identity with the mouse sequence and 88% identity with the rat sequence.
Localization:	Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells

Entrez:	8678 (Human)
Swiss Prot:	Q14457 (Human)
Related Genes:	BCL2, PIK3CG, ANXA3, ATG5, MTOR, CASP3, BCL2L1, AKT1, ATG7, PIK3C3, UVRAG, NKX3-1, BAX, PIK3R1, PIK3CB, PIK3CD, TNF, MAPK8, MAP1LC3B, MAPK3, ATG12, PCBD1, DNAH5, DNAI1, TRP53, AGTPBP1, TP53, HSPA5, BEC-1, NFKB1, PARP1, CASP8, CTSD, EPHB2, RFC1, ATP6V0A1, BNIP3, SH3GLB1, RFC2, BCL2A1A

Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.

Reactivity:	This antibody can be used for detection of Beclin 1 in human samples. Preliminary results suggest that this antibody may cross-react with mouse protein, as well.

Uses:	Western analysis where a band is seen at ~52 kDa representing Beclin 1. Preliminary results suggest that this antibody may be used for immunofluorescence, as well (tested only on mouse tissue). Has also been used for immunohistochemistry (paraffin) and immunoprecipitation.
Dilutions:	immunohistochemistry , immunofluorescence , immunoprecipitation , Western Blot 1:2000 - 1:5000, Immunohistochemistry-Paraffin ,
Concentrations:	1.0 mg/ml
Conjugated Ab's:	4 conjugated antibodies

Buffer:	50mM sodium borate
Storage:	Store at 4C. Do not freeze.
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.