Beclin 1 Antibody (NB500-249)
Beclin 1 Antibody
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Distributor Data...
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anti-Becl antibody, anti-ATG 6 antibody, anti-VPS30 antibody, anti-Protein GT197 antibody, anti-CTIP-1 COUP-TF interacting protein 1 antibody, anti-Coiled-coil myosin-like BCL2-interacting protein antibody, anti-BECN1 antibody, anti-BECN 1 antibody, anti-Beclin1 autophagy antibody, anti-Beclin-1 antibody, anti-beclin1 antibody, anti-Beclin 1 antibody, anti-ATG6 antibody, anti-ATG 6 antibody, anti-APG6 antibody, |
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Beclin 1 Antibody Summary: | | Gene: | BECN1 |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | Specificity: | This is specific to Beclin 1. |
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| Publications: | Antibody has been mentioned in at least 10 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Beclin 1 Products Beclin 1 Antibody Details: | Immunogen: | NB 500-249 was raised against an internal synthetic peptide to human Beclin 1, containing residues within 1-100. This sequence has 94% identity with the mouse sequence and 88% identity with the rat sequence.
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| Localization: | Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells |
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This antibody can be used for detection of Beclin 1 in human and mouse samples. |
| Uses: | Western blot, Immunoprecipitation, Immunocytochemistry and most histochemical applications. Western blot a band is seen at ~52 kDa representing Beclin 1. |
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| Dilutions: | Immunocytochemistry 1:50-1:200, Immunofluorescence 1:50-1:200, Immunohistochemistry, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 1.0 mg/ml |
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| Positive Controls: | 1 Positive Controls |
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| Conjugated Antibodies: | 5 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | Tris-citrate/phosphate, pH 7-8 |
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| Preservative: | 0.1% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.
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Images (3) Reviews (1) Related Products (44) Exploring Beclin 1 Working with Beclin 1
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with Beclin 1
Beclin 1 Antibody Images (3) Immunoctyochemistry: Beclin 1 Antibody [NB500-249] - Beclin 1 staining in Hela cells detected with a Dylight 488 labeled secondary antibody using NB500-249. |  Immunohistochemistry: Beclin 1 Antibody [NB-500-249] - Pheochromocytes of the Adrenal Medulla 40x |  Western Blot: Beclin 1 Antibody [NB-500-249] - Detection of Beclin in mouse liver tissue lysate (50 ug) using NB 500-249. ECL detection at 1 minute. |
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Reviewed by:
Amy Exum 9/28/2010
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Application:
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IHC
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Sample Tested:
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Human Esophagus tissue
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Species:
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Human
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Sample Preparation:
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FFPE
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Sample Pretreatment:
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na
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Visualization Method:
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dab
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Did you use controls?:
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Yes
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Publications (10)Beclin 1 Antibody Product Specific:- Cheng H-C, Kim SR, Oo TF, et al. Akt suppresses retrograde degeneration of dopaminergic axons by inhibition of macroautophagy. J. Neurosci. 2011 Feb 9. [PMID: 21307249]
- Belibi F, Zafar I, Ravichandran K, et al. Hypoxia-inducible factor 1{alpha} (HIF-1 {alpha}) and autophagy in polycystic kidney disease (PKD). Am J Physiol Renal Physiol. 2011. [PMID 21270095]
more... - Monaco-Shawver L, Schwartz L, Tuluc F, et al. Substance P inhibits natural killer cell cytotoxicity through the neurokinin-1 receptor.J Leukoc Biol. 2011 Jan;89(1):113-25.[PMID: 20940324]
- Zhang H, Bosch-Marce M, Shimoda LA, et al. Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia. J Biol Chem. 2008 Apr 18;283(16):10892-903. [PMID: 18281291] (Mouse, WB)
- Pattingre S, Bauvy C, Carpentier S, et al. Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy. J Biol Chem. 2009 Jan 30;284(5):2719-28. [PMID: 19029119] (Human, WB)
- Gulati P, Gaspers LD, Dann SG, et al. Amino acids activate mTOR complex 1 via Ca2+/CaM signaling to hVps34. Cell Metab. 2008 May;7(5):456-65. [PMID: 18460336] (Human, WB)
- Massey, A. C., Follenzi, A., Kiffin, R., et al. Early cellular changes after blockage of chaperone-mediated autophagy. Autophagy. May 16, 2008; 4:4, 442-456. [PMID: 18253088] (Mouse, WB)
- Wang, Y., R. Singh, et al. Loss of Macroautophagy Promotes or Prevents Fibroblast Apoptosis Depending on the Death Stimulus. J. Biol. Chem. 2008; 283: 4766-4777. [PMID: 18073215] (Mouse, WB)
- Park, K. et al. A Human scFv Antibody against TRAIL Receptor 2 Induces Autophagic Cell Death in Both TRAIL-Sensitive TRAIL-Resistant Cancer Cells. Cancer Res., Aug 2007; 67: 7327 - 7334. [PMID: 17671202] (Human, WB/IP)
- Personal communications with Dr. Beth Levine, Columbia University.
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Jump to: Conjugated Antibodies, Positive Controls, Antibody Packs, Lysates, Peptides and Proteins, Primary Antibodies, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Beclin 1 Antibody [Biotin] | NB500-249B | Hu, Mu | IF, IHC, IHC-P, IP, WB | (2) | (6) | | | | Beclin 1 Antibody [HRP] | NB500-249H | Hu, Mu | IF, IHC, IHC-P, IP, WB | (2) | (6) | | | | Beclin 1 Antibody [DyLight 488] | NB500-249G | Hu, Mu | IF, IHC, IHC-P, IP, WB | | (7) | | | | Beclin 1 Antibody [DyLight 550] | NB500-249R | Hu, Mu | IF, IHC, IHC-P, IP, WB | | (7) | | | | Beclin 1 Antibody [DyLight 650] | NB500-249C | Hu, Mu | IF, IHC, IHC-P, IP, WB | | (7) | | |
Positive ControlsAntibody Packs (2)Lysates (1)Peptides and ProteinsPrimary Antibodies (39)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Beclin 1 Antibody | NB110-87318 | Bv, Ch, Hu, Mk, Mu, Po, Rt, Xp | IHC-P, WB | (4) | (4) | | | | Beclin 1 Antibody (9B6) | NBP1-00088 | Bv, Ch, Eq, Hu, Mk, Mu, Po, Rt | IHC, WB | (1) | (5) | | | | Beclin 1 Antibody (1B7) | NBP1-00084 | Bv, Ch, Eq, Hu, Mk, Mu, Po, Rt | IHC-P, WB | (1) | (5) | | | | Beclin 1 Antibody (4H10) | NBP1-00085 | Bv, Ch, Eq, Hu, Mk, Mu, Po, Rt | IHC-P, WB | (1) | (5) | | | | Beclin 1 Antibody | NB500-266 | Hu, Mu | WB | (1) | (5) | | | | ... and 34 more. See All » |
Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | Rabbit IgG Antibody [Cy3] | NB120-6939 | Rb | ELISA, FACS, ICC, IF, IHC-Fr, IHC-P | | (1) | | | | See All » |
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