Bcl2 Antibody (NB300-900)

Bcl2 Antibody

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Bcl2 Antibody Summary:
Species:Hu
Applications:PEP-ELISA, WB
Clonality:Polyclonal
Gene:BCL2
Purity:Immunogen affinity purified
Host:Goat
Specificity:This antibody is expected to recognise both reported isoforms (NP_000624; NP_000648)
Publications:Antibody has been mentioned in at least 2 Publications.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Bcl2 Antibody Details:
Isotype:IgG
Immunogen:Synthetic peptide AGRTGYDNREIVMKYC, representing the N-Terminus of the human protein sequence according to NP_000624; NP_000648.
Species Reactivity:

Human, Mouse, Rat, Dog

Applications:
Uses:This antibody is useful for Peptide ELISA and Western Blot. Approx 25 kDa band observed in Human Lymph node lysates (predicted MW of 26kDa according to NP_000624, alpha isoform).
Dilutions:Peptide ELISA 1:2000, Western Blot 1:1000
Unit Size:0.1 mg
Concentration:0.5 mg/ml
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:Tris-saline [pH7.3] with 0.5% BSA
Preservative:0.02% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: BCL2
Entrez596 (Human)
12043 (Mouse)
24224 (Rat)
Swiss ProtNP_000624; NP_000648.
 
Background:

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

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Working with Bcl2

Bcl2 Antibody Images (1)
EB06444 staining (1ug/ml) of Human Lymph Node lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Publications (2)

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Jump to: Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Bcl2 in Context


Research Areas related to Bcl2 (5):
Novus Explorer for Bcl2 gene

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