Bcl2 Antibody (NB100-81810)

Bcl2 Antibody

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Bcl2 Antibody Summary:
Species:Hu
Applications:IF, IHC-P, WB
Clonality:Polyclonal
Gene:BCL2
Purity:Immunogen affinity purified
Host:Rabbit
Specificity:BCL-2(specific to a.a. 70) detects endogenous levels of total BCL-2 protein.
Publications:Antibody has at least 3 Background References.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Bcl2 Antibody Details:
Isotype:IgG
Immunogen:The antiserum was produced against synthesized non-phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-SP-P-L).
Species Reactivity:

Human

Applications:
Uses:Immunofluorescence, Western Blot and Immunohistochemistry-Paraffin
Dilutions:Immunofluorescence 1:100-1:200, Immunohistochemistry-Paraffin, Western Blot 1:500-1:1000
Unit Size:0.1 mg
Concentration:1.0 mg/ml
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative:0.02% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: BCL2
Entrez596 (Human)
Swiss ProtP10415 (Human)
 
Background:

Bcl2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl2, such as in the case of translocation of Bcl2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C terminal ends.

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Working with Bcl2

Bcl2 Antibody Images (2)
Immunohistochemistry: BCL-2 Antibody [NB100-81810] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 (Ab-70) antibody.Western Blot: BCL-2 Antibody [NB100-81810] - Western blot analysis of extracts from MCF7 cells using BCL-2 (Ab-70) antibody.
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Publications (3)

Bcl2 Antibody Background References:

  1. Huang ST,et al. (2002)FASEB J; 16(8): 825-32. Yamamoto K,et al. (1999) Mol Cell Biol; 19(12): 8469-78.
  2. Kumar Biswas S,et al. Mol Cancer Ther 2004 Mar; 3(3): 327-34.

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Jump to: Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Bcl2 in Context


Novus Explorer for Bcl2 gene

Try our bioinformatic tool that shows the relationships between BCL2 and other genes, diseases, pathways and post-translational modifications.