Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The antiserum was produced against synthesized non-phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 56 (G-H-TP-P-H).
Species Reactivity:
Human
Applications:
Uses:
Immunofluorescence, Western Blot and Immunohistochemistry-Paraffin
Dilutions:
Immunofluorescence 1:100-1:200, Immunohistochemistry-Paraffin, Western Blot 1:500-1:1000
Unit Size:
0.1 mg
Concentration:
1.0 mg/ml
Packaging:
Storage:
Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative:
0.02% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Bcl2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl2, such as in the case of translocation of Bcl2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C terminal ends.
Immunohistochemistry: BCL-2 Antibody [NB100-81809] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using BCL-2(Ab-56) antibody.
Western Blot: BCL-2 Antibody [NB100-81809] - Western blot analysis of extracts from MCF7 cells treated with etoposide using BCL-2(Ab-56) antibody.