Bcl2 Antibody (NB110-55551)
Bcl2 Antibody (E17) - Neuronal Apoptosis Inhibitor
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anti-B cell CLL/lymphoma 2 antibody, anti-Apoptosis regulator Bcl 2 antibody, anti-BCL2 protein antibody, anti-Bcl2 antibody, anti-Bcl 2 antibody, anti-B cell lymphoma 2 antibody, anti-B cell leukemia/lymphoma 2 antibody, anti-Apoptosis regulator Bcl2 antibody, |
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Bcl2 Antibody (E17) - Neuronal Apoptosis Inhibitor Summary: | | Gene: | BCL2 |
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| Purity: | IgG purified |
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| Host: | Rabbit |
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| | Specificity: | The antibody does not cross-react with other Bcl-2 family members |
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| Publications: | Antibody has at least 1 Background Reference. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Bcl2 Products Bcl2 Antibody (E17) - Neuronal Apoptosis Inhibitor Details: | Clone: | E17 |
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| Isotype: | IgG |
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| Immunogen: | A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2 was used as immunogen. |
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| Localization: | Endoplasmic reticulum, outer mitochondrial membrane and intracellular membrane of the nuclear envelope. |
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| Uses: | This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 26kDa. |
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| Dilutions: | Western Blot 1:500, Immunohistochemistry-Paraffin 1:100-1:200, Immunocytochemistry 1:200, Immunoprecipitation 1:50, Flow Cytometry 1:200 |
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| TMW: | 26 kDa |
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| Unit Size: | 0.1 ml |
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| Concentration: | Please see the vial label for concentration. |
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| Positive Controls: | 3 Positive Controls |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).
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Images (3) Reviews (0) Related Products (43) Working with Bcl2
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Jump to: Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive ControlsPeptides and ProteinsPrimary Antibodies (25)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Bcl2 Antibody (8C8) - Neuronal Apoptosis Inhibitor | NB200-572 | Hu, Mk, Mu(-), Rt(-) | IHC-Fr, IHC-P, WB | (2) | (1) | | | | Bcl2 Antibody | NBP1-72199 | Bv, Ca, Hu, Mu, Rt | ELISA, IHC-P, IP, WB | (2) | (1) | | | | Bcl2 Antibody | NBP1-61508 | Hu, Mu, Rt | ELISA, IF, IHC-P, WB | (3) | | | | | Bcl2 Antibody | NB100-92142 | Hu, Mu, Rt | IF, IHC-P, WB | (3) | | | | | Bcl2 [p Thr129] Antibody | NBP1-72213 | Bv, Ca(-), Hu, Mu, Rt | ELISA, IHC-P, IP, WB | (1) | (1) | | | | ... and 20 more. See All » |
RNAi (2)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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![Flow Cytometry: Bcl-2 Antibody (E17) [NB110-55551] - Analysis of permeabilized Jurkat cells using anti-Bcl-2 (red)(catalog #1017-1)or a rabbit IgG (negative) (green).](http://images.novusbio.com/images/110-55551_3.jpg "Flow Cytometry: Bcl-2 Antibody (E17) [NB110-55551] - Analysis of permeabilized Jurkat cells using anti-Bcl-2 (red)(catalog #1017-1)or a rabbit IgG (negative) (green).")
![Immunohistochemistry: Bcl-2 Antibody (E17) [NB110-55551] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-BCL-2 Rab Mab](http://images.novusbio.com/images/p_55551_2.jpg "Immunohistochemistry: Bcl-2 Antibody (E17) [NB110-55551] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-BCL-2 Rab Mab")
![Western Blot: Bcl-2 Antibody (E17) [NB110-55551] - Western blot analysis of Jurkat cell lysate using anti-Bcl-2 (NB110-55551), 1:1,000 dilution.](http://images.novusbio.com/images/p_55551_1.jpg "Western Blot: Bcl-2 Antibody (E17) [NB110-55551] - Western blot analysis of Jurkat cell lysate using anti-Bcl-2 (NB110-55551), 1:1,000 dilution.")
