Bcl2 Antibody (NB200-572)

Bcl2 Antibody (8C8) - Neuronal Apoptosis Inhibitor

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Bcl2 Antibody (8C8) - Neuronal Apoptosis Inhibitor Summary:
Species:Hu, Mk, Mu(-), Rt(-)
Applications:IHC-Fr, IHC-P, WB
Clonality:Monoclonal
Gene:BCL2
Purity:Protein G purified
Host:Mouse
Specificity:This recognizes a protein of 25-26kDa, identified as the bcl-2a oncoprotein. It does not cross-react with bcl-x or bax protein.
Publications:Antibody has been mentioned in at least 1 Publication.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Bcl2 Antibody (8C8) - Neuronal Apoptosis Inhibitor Details:
Clone:8C8
Isotype:IgG1
Immunogen:A Synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys), of human bcl-2 protein.
Localization:Cytoplasmic
Epitope:aa 41-54
Species Reactivity:

Cross-reacts with Human and Monkey. Does not cross-react with Rat or Mouse. Not yet tested in other species.

Applications:
Uses:For western blot use for 2hrs at RT. Has also been used for immunohistochemistry on paraffin sections
Dilutions:Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 1-2 ug/ml, Western Blot 1 ug/ml
Unit Size:0.5 ml
Concentration:0.2 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Store at 4 °C. Do not freeze.
Buffer:10mM PBS, pH 7.4, with 0.2% BSA
Preservative:0.09% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: BCL2
Entrez596 (Human)
12043 (Mouse)
24224 (Rat)
 
Background:

Expression of bcl-2a oncoprotein has been shown to inhibit the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2a protein, whereas the normal or hyperplastic germinal centers are negative.

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Working with Bcl2

Bcl2 Antibody (8C8) - Neuronal Apoptosis Inhibitor Images (2)
Formalin-fixed, paraffin-embedded human tonsil stained with (NB 200-572) using peroxidase-conjugate and DAB chromogen. Note absence of staining of reactive cells in the germinal center. Western Blot: Bcl2 Antibody (8C8) - Neuronal Apoptosis Inhibitor [NB200-572]
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Publications (1)

Bcl2 Antibody (8C8) - Neuronal Apoptosis Inhibitor Product Specific:

  1. Wolter KG, et. al. J Cell Bio, 1997, 139(5):1281-92.

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Jump to: Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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Positive Controls
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Bcl2 AntibodyNBP1-61508Hu, Mu, RtELISA, IF, IHC-P, WB(3)

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RNAi (2)
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Bcl2 RNAiH00000596-R02Hu(1)(4)

Bcl2 RNAiH00000596-R01Hu(1)(4)

Secondary Antibodies
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Mouse IgG Antibody [Biotin]NB720-BMuELISA, IHC-P, WB

Mouse IgG Antibody [HRP]NB720-HMuELISA, ICC, IHC-P, WB

Mouse IgG Antibody [Alkaline Phosphatase]NB720-APMuELISA, ICC, IHC-P, WB

Mouse IgG Antibody [FITC]NB720-FMuICC, IHC-P(2)

Mouse IgG AntibodyNB120-7056Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-)ELISA, IHC-P, IP, WB

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Bcl2 in Context


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