BNIP3 Antibody

Species: Hu Applications: WB, IHC Clonality: Polyclonal	Gene: BNIP3 Purity: Whole antisera Host: Rabbit
Specificity: Appears to be specific for BNIP3.
See All BNIP3 Products See All BNIP3 Related Products: Lysates Peptides and Proteins Primary Antibodies
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Immunogen:	A synthetic peptide from the human phospho S95 BNIP3 (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, NIP3) conjugated to an immunogenic carrier protein was used as the immunogen.

Entrez:	664 (Human)
Related Genes:	BCL2, BNIP3L, CASP3, BCL2L1, HIF1A, VEGFA, BBC3, SETD2, NKX3-1, BAX, TRP53, TP53, CASP9, EPAS1, AKT1, CDKN1A, BID, BECN1, SLC2A1, BAK1, TNF, NFKB1, AIFM1, CAR9, CA9, PARP1, ARNT, MAP1LC3A, BCKDHB, BCL2L11, ANXA3, MTOR, NOS2, BIK, BCL2L2, ENDOG, DIABLO, PMAIP1, APAF1, FAS

BNIP3, formerly NIP3 (nineteen kDa interacting protein-3), is a pro-apoptotic, mitochondrial protein classified in the Bcl 2 family based on limited sequence homology to the Bcl 2 homology 3 (BH3) domain (amino acids 110-118) and C-terminal TM domain. BNIP3 expressed in yeast and mammalian cells interacts with survival promoting proteins Bcl 2, Bcl XL, CED9 and the adenovirus E1B 19K protein. Typically the BH3 domain of pro-apoptotic Bcl-2 homologues mediates Bcl 2/Bcl XL heterodimerization and confers pro-apoptotic activity. BNIP3 represents a subfamily of Bcl 2 related proteins, which functions without a typical BH3 domain to regulate apoptosis from both mitochondrial and nonmitochondrial sites by selective Bcl 2/Bcl XL interactions. The N-terminus (residues 1-49) and the C-terminus TM domain of BNIP3 are critical for Bcl 2 heterodimerization, and either region is sufficient for Bcl XL interaction. The TM domain of BNIP3 is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting. BNIP3 contains PEST sequences suggesting that the protein may be susceptible to rapid degradation by proteases. PEST sequences commonly contain high local concentrations of amino acids P, E, S, T, and D flanked by charged amino acids and these are abundantly present in NIP3. Thus, the posttranslational control of BNIP3 expression through rapid protein degradation may constitute a mechanism for regulating the intracellular levels of a potentially lethal protein.

Reactivity:	Human. Other species not yet tested.

Uses:	IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. This antibody may also recognise the unphosphorylated form of the BNIP3.
Dilutions:	Immunohistochemistry, Western Blot 1:300-1:2000

Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Buffer:	Lyophilised
Storage:	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.