BLIMP1/PRDM1 Antibody (3H2-E8) 0.1 ml Plasma Cell Marker

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Western Blot: Blimp-1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line). (Tedder Lab; Duke Univ)
Immunocytochemistry/Immunofluorescence: Blimp-1 Antibody (3H2-E8) [NB600-235] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um).  Positive surface epithelium and centrocytes are labelled.   Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.
Flow Cytometry: Blimp-1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ)
Western Blot: Blimp-1 Antibody (3H2-E8) [NB600-235] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using NB 600-235. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.
Flow Cytometry: Blimp-1 Antibody (3H2-E8) [NB600-235] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ).
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Ordering Information: NB600-235

  • Catalog Number
    NB600-235
  • Displayed Format
    Unconjugated
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BLIMP1/PRDM1 Antibody (3H2-E8) Summary

Species Human, Mouse, Porcine
Tested Applications WB, ChIP, ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Monoclonal
Host
Mouse
Gene
PRDM1
Purity
Protein G purified
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BLIMP1/PRDM1 Antibody (3H2-E8) Details

Clone
3H2-E8
Isotype
IgG1
Marker
Plasma Cell Marker
Immunogen
A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]
Localization
Nuclear

Species Reactivity

Human, mouse and pig.
Reviewed Species Read 2 rated 3.5
using NB600-235 in the following species:

Publications Read Publications using NB600-235 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 1ug antibody / 10 ug protein
  • ELISA 1:100-1:2000
  • Flow Cytometry 8 ug/ml where cells are used at 2*10^6/mL
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
Application Notes
This Blimp-1 (3H2-E8) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation and Chromatin Immunoprecipitation applications. Immunohistochemistry-Frozen was reported in scientific literature. By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.
Positive Controls
Other Available
Formats
7C Labeled NB600-235V2
Alexa Fluor (R) 405 Labeled NB600-235AF405
Alexa Fluor (R) 488 Labeled NB600-235AF488
Alexa Fluor (R) 647 Labeled NB600-235AF647
Alexa Fluor (R) 700 Labeled NB600-235AF700
Allophycocyanin Labeled NB600-235APC
Biotin Labeled NB600-235B
DyLight 350 Labeled NB600-235UV
DyLight 405 Labeled NB600-235V
DyLight 405LS Labeled NB600-235V3
DyLight 488 Labeled NB600-235G
DyLight 550 Labeled NB600-235R
DyLight 650 Labeled NB600-235C
DyLight 680 Labeled NB600-235FR
DyLight 755 Labeled NB600-235IR
FITC Labeled NB600-235F
HRP Labeled NB600-235H
PE Labeled NB600-235PE
PerCP Labeled NB600-235PCP
Reviewed Applications
Read 2 Review rated 3.5
using
NB600-235 in the following applications:

Publications
Read Publications using
NB600-235 in the following applications:

Contact Information

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Publications

BLIMP1/PRDM1 Antibody (NB600-235) has been mentioned in at least 33 publications.
Earn rewards if you have published using BLIMP1/PRDM1 Antibody (NB600-235).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-glycine, 150 mM NaCl
Unit Size
0.1 ml
Concentration
1.2 mg/ml
Preservative
0.1% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol PRDM1
Entrez
Uniprot

Alternate Names for BLIMP1/PRDM1 Antibody (3H2-E8)

  • Beta-interferon gene positive regulatory domain I-binding factor
  • beta-interferon gene positive-regulatory domain I binding factor
  • BLIMP-1
  • BLIMP1MGC118925
  • B-lymphocyte-induced maturation protein 1
  • MGC118922
  • MGC118923
  • Positive regulatory domain I-binding factor 1
  • PR domain containing 1, with ZNF domain
  • PR domain zinc finger protein 1
  • PR domain-containing protein 1
  • PRDI-BF1MGC118924
  • PRDI-binding factor 1
  • PRDI-binding factor-1
  • PR-domain zinc finger protein 1
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Related Products by Gene

Background

Blimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor which primarily controls the terminal differentiation of mature B cells to plasma cells, and also involves in macrophage as well as T-cells population homeostasis/differentiation. It is also called positive regulatory domain I-binding factor-1 (PRDI-BF1) or PR (PRDI-BF1-RIZ) domain zinc finger protein 1 (PRDM1) and its first human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-beta promoter leading to inhibition of virus-mediated IFN-beta production. Blimp-1 contains N-terminal PR/SET domain and five C2H2 zinc fingers located near its C-terminus that mediate DNA binding, nuclear import and recruitment of histone modifying enzymes, and these activities enable Blimp-1 for its ability to control cell-fate decisions in the embryo and govern tissue homeostasis in multiple cell types in the adult organism. Blimp-1 recruits chromatin-modifying enzymes including HDACs and methyltransferases, and some of its target genes include c-Myc, CIITA, Pax5, Spi-B, and Id3. Blimp-1 sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Deletion mutation of Blimp-1 is frequently observed in several tumors including lymphoid malignancies and has been proposed to be a candidate of tumor suppressor gene.

Reviews for BLIMP1/PRDM1 Antibody (NB600-235) (2) 3.52

Read what people are saying who have used BLIMP1/PRDM1 Antibody (NB600-235).

We have 2 reviews tested in 1 species: Other.
We have 2 reviews tested in 2: Immunohistochemistry-Paraffin, Immunocytochemistry/Immunofluorescence.
Average Rating: 3.5
(Based on 2 reviews)

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Sort 2 reviews for BLIMP1/PRDM1 Antibody (NB600-235) by:
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Very Good
(1)
Average
(1)
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IHC-P
(1)
ICC/IF
(1)
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(2)
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Images Ratings Applications Species Reviewed By Date Details
  Very Good
IHC-P Other Anne Friis Petersen 05/31/2010
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPorcine embryo
SpeciesOther
Other SpeciesPorcine
CommentsI stained paraffin embedded porcine embryonic tissue to verify the usefulness of Blimp1 as a germline marker. The staining was highly specific (nuclear), but only successful when using chromogenic development (used the HRP-based AEC-red system). Citrate buffer was superior to EDTA for antigen retrieval and 1:500 dilution in 0.05M Tris-HCl, pH 6.0 with 1% BSA was superior to higher concentrations and higher pH or PBS-based solution.  Detection Method: AEC red. Negative Control: no primary and isotype control. Deparaffinization: 2x10min xylene, 2x 5min 99% etOH, 3min 96% etOH, 3min 70% etOH, 3 min water. Antigen Retrieval: 0.01M citrate buffer, 15min at boiling.

Blocking

Blocking DetailsBlocking Buffer: 2% BSA, Blocking Time: 10 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: 0.05M Tris-HCL, pH 6.0, 1 % BSA, Incubation Time: 1hr, Temp: RT

Details

Detection NotesPlease see comments
Fixation DetailsPFA fixed and paraffin embedded, Deparaffinization: 2x 10min xylene, 2x 5min 99% etOH, 3min 96% etOH, 3min 70% etOH, 3min water

Comments

CommentsI stained paraffin embedded porcine embryonic tissue to verify the usefulness of Blimp1 as a germline marker. The staining was highly specific (nuclear), but only successful when using chromogenic development (used the HRP-based AEC-red system). Citrate buffer was superior to EDTA for antigen retrieval and 1:500 dilution in 0.05M Tris-HCl, pH 6.0 with 1% BSA was superior to higher concentrations and higher pH or PBS-based solution.  Detection Method: AEC red. Negative Control: no primary and isotype control. Deparaffinization: 2x10min xylene, 2x 5min 99% etOH, 3min 96% etOH, 3min 70% etOH, 3 min water. Antigen Retrieval: 0.01M citrate buffer, 15min at boiling.
  Average
ICC/IF Other x x 02/17/2009
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedBlimp1
SpeciesOther
Other SpeciesOther
LotSS

Blocking

Blocking DetailsBlocking Buffer: BSA, Blocking Time: 1 hour, Blocking Temp: 37 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: 0.5% BSA, Primary Ab Dilution Ratio: 1/200, Incubation Time:1.5 hr, Incubation Temp:37C

Publications for BLIMP1/PRDM1 Antibody (NB600-235) (33)

Read what people are writing who have used BLIMP1/PRDM1 Antibody (NB600-235).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: WB, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP.

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Filter By Application
WB
(13)
FLOW
(3)
ICC/IF
(3)
IHC
(1)
IHC-Fr
(1)
IHC-P
(6)
IP
(2)
All Applications
Filter By Species
Human
(13)
Mouse
(11)
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Publication Applications Species
John, S A et al. Ets-1 regulates plasma cell differentiation by interfering with the activity of the transcription factor Blimp-1. J Biol Chem;283(2):951-62. 2008 Jan 11. [PMID: 17977828] (WB, Mouse) WB Mouse
Nishikawa K, Nakashima T, Hayashi M et al. Blimp1-mediated repression of negative regulators is required for osteoclast differentiation. Proc Natl Acad Sci U S A 2010 Feb 16. [PMID: 20133620]
Maseda D, Smith SH, Dilillo DJ et al. Regulatory B10 Cells Differentiate into Antibody-Secreting Cells After Transient IL-10 Production In Vivo. Journal of Immunology 2011 Dec 23. [PMID: 22198952]
Severa, M et al. Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1. J Biol Chem;281(36):26188-95. 2006 Sep 8. [PMID: 16849320]
Gong, D, Malek, T R. Cytokine-dependent Blimp-1 expression in activated T cells inhibits IL-2 production. J Immunol;178(1):242-52. 2007 Jan 1. [PMID: 17182561]
Liu, Y et al. Rituximab plus CHOP (R-CHOP) overcomes PRDM1-associated resistance to chemotherapy in patients with diffuse large B-cell lymphoma. Blood;110(1):339-44. 2007 Jul 1. [PMID: 17379744] (WB, IHC-P, Human) WB, IHC-P Human
Garcia-Bates TM, Baglole CJ, Bernard MP et al. Peroxisome proliferator-activated receptor gamma ligands enhance human B cell antibody production and differentiation. J Immunol;183(11):6903-12. 2009 Dec 1. [PMID: 19915048]
Julaton VTA, Reijo Pera RA. NANOS3 function in human germ cell development. Hum Mol Genet;20(11):2238-50. 2011 Jun 1. [PMID: 21421998] (ICC/IF, Human) ICC/IF Human
Shimshon L, Michaeli A, Hadar R et al. SUMOylation of Blimp-1 promotes its proteasomal degradation. FEBS Lett;585(15):2405-9. 2011 Aug 4. [PMID: 21722636] (IP, Mouse) IP Mouse
Gao Y, Kazama H, Yonehara S. Bim regulates B-cell receptor-mediated apoptosis in the presence of CD40 signaling in CD40-pre-activated splenic B cells differentiating into plasma cells. Int Immunol. 2012 Feb 1. [PMID: 22301689]
Altin JA, Goodnow CC, Cook MC. IL-10+CTLA-4+ Th2 Inhibitory Cells Form in a Foxp3-Independent, IL-2-Dependent Manner from Th2 Effectors during Chronic Inflammation. J Immunol;188(11):5478-88. 2012 Jun 1. [PMID: 22547705] (FLOW, Mouse) FLOW Mouse
Cattoretti, G et al. PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage. J Pathol;206(1):76-86. 2005 May. [PMID: 15772984] (IHC-P, WB, Human) WB, IHC-P Human
Robertson EJ, Charatsi I, Joyner CJ et al. Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice. Development;134(24):4335-45. 2007 Dec. [PMID: 18039967] (ICC/IF, Mouse) ICC/IF Mouse
Courts C, Montesinos-Rongen M, Brunn A, Bug S, Siemer D, Hans V, Blumcke I, Klapper W, Schaller C, Wiestler OD, Kuppers R, Siebert R, Deckert M. Recurrent inactivation of the PRDM1 gene in primary central nervous system lymphoma. J Neuropathol Exp Neurol;67(7):720-7. 2008 Jul. [PMID: 18596541] (IHC-P, IHC-Fr, Human) IHC-P, IHC-Fr Human
Chang, DH et al. The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development. Mech Dev 1179(1-2): 305-309. 2002 [PMID: 12204275] (IHC-P, Mouse) IHC-P Mouse
Wang X, Belguise K, O'Neill CF et al. RelB NF-{kappa}B Represses Estrogen Receptor {alpha} Expression via Induction of the Zinc Finger Protein Blimp1. Mol Cell Biol;29(14):3832-3844. 2009 [PMID: 19433448]
Matsushita T, Tedder TF. Identifying regulatory B cells (B10 cells) that produce IL-10 in mice. Methods Mol Biol;677:99-111. 2011 [PMID: 20941605]
Le Gallou S, Caron G, Delaloy C et al. IL-2 Requirement for Human Plasma Cell Generation: Coupling Differentiation and Proliferation by Enhancing MAPK-ERK Signaling J Immunol 2012 Jul 1 [PMID: 22634617] (WB, Human) WB Human
Ramon S, Gao F, Serhan CN, Phipps RP. Specialized Proresolving Mediators Enhance Human B Cell Differentiation to Antibody-Secreting Cells J Immunol 2012 Jun 18 [PMID: 22711890] (WB, Human) WB Human
Takata K, Sato Y, Nakamura N et al. Duodenal follicular lymphoma lacks AID but expresses BACH2 and has memory B-cell characteristics Mod Pathol 2012 Aug 17 [PMID: 22899287] (IHC, Human) IHC Human
Morgan MA, Mould AW, Li L et al. Alternative splicing regulates prdm1/blimp-1 DNA binding activities and corepressor interactions Mol Cell Biol 2012 Sep [PMID: 22733990] (IP, Human) IP Human
Lee SR, Rutan JA, Monteith AJ et al. Receptor Cross-Talk Spatially Restricts p-ERK during TLR4 Stimulation of Autoreactive B Cells J Immunol 2012 Sep 14 [PMID: 22984080] (WB, Mouse) WB Mouse
Lin MH, Chou FC, Yeh LT et al. B lymphocyte-induced maturation protein 1 (BLIMP-1) attenuates autoimmune diabetes in NOD mice by suppressing Th1 and Th17 cells Diabetologia 2012 Oct 7 [PMID: 23052053] (WB, Mouse) WB Mouse
Nagy N, Adori M, Rasul A et al. Soluble factors produced by activated CD4+ T cells modulate EBV latency Proc Natl Acad Sci U S A 2012 Jan 31 [PMID: 22307606] (WB, Human) WB Human
fan CC, Lee LY, Yu MY et al. Upregulated hPuf-A promotes breast cancer tumorigenesis. Tumour Biol 2013 Apr 28 [PMID: 23625657]
Wong LY, Brulois K, Toth Z et al. KSHV K4.2 immediate early gene product regulates immunoglobulin secretion and calcium homeostasis by interacting with and inhibiting pERP1. J Virol. 2013 Aug 28 [PMID: 23986581] (IHC-P, Human) IHC-P Human
Parlato S, Bruni R, Fragapane P et al. IFN-alpha Regulates Blimp-1 Expression via miR-23a and miR-125b in Both Monocytes-Derived DC and pDC. PLoS One. 2013 Aug 16 [PMID: 23977359] (WB, Human) WB Human
Kim EH, Gasper DJ, Lee SH et al. Bach2 regulates homeostasis of Foxp3+ regulatory T cells and protects against fatal lung disease in mice. J. Immunol. 2014 Feb 1 [PMID: 24367030] (FLOW, Mouse) FLOW Mouse
Severa M, Islam SA, Waggoner SN et al. The Transcriptional Repressor BLIMP1 Curbs Host Defenses by Suppressing Expression of the Chemokine CCL8. J. Immunol. 2014 Feb 24 [PMID: 24477914] (WB, Mouse) WB Mouse
Horn M, Geisen C, Cermak L et al. DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. elegans Developmental Timing and Maturation. Dev. Cell 3/4/2014 [PMID: 24613396] (WB, Human) WB Human
Kim J, Nakasaki M, Todorova D et al. p53 induces skin aging by depleting Blimp1(+) sebaceous gland cells. Cell Death Dis 3/28/2014 [PMID: 24675459] (IHC-P, ICC/IF, FLOW, Mouse) FLOW, ICC/IF, IHC-P Mouse
Rasul E, Salamon D, Nagy N et al. The MEC1 and MEC2 Lines Represent Two CLL Subclones in Different Stages of Progression towards Prolymphocytic Leukemia. PLoS ONE. 2014 Aug 28 [PMID: 25162594] (WB, Human) WB Human
Lin MH, Yeh LT, Chen SJ et al. T cell-specific BLIMP-1 deficiency exacerbates experimental autoimmune encephalomyelitis in nonobese diabetic mice by increasing Th1 and Th17 cells. Clin Immunol. 2014 Mar 10 [PMID: 24568746] (WB, Mouse) WB Mouse

Product General Protocols

View specific protocols for BLIMP1/PRDM1 Antibody (NB600-235): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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FAQs for BLIMP1/PRDM1 (6)

  1. Does your Blimp-1 Antibody (NB600-235) recognize both the alpha and beta form of BLIMP-1
    • By WB, this antibody recognizes a band ~98 kD and may recognize one ~80 kDa (the beta form).
  2. Do you have any images (dot plot chart or histogram) of human cells stained with this ab for flow cytometry?
    • Unfortunately, I do not have any additional images for this product on my records. This is one of our best selling products with great customer feedback and citations in at least 24 peer reviewed publications in journals of high repute. We stand by the quality of our products and all our products are 100% guaranteed. We are always there to help in case you face any trouble in generating your desired outcome out of the use of our products.
  3. Do you have any references which show this works in human for IF?
    • The research publication that has evidence for IF use of the above mentioned antibody in samples of human origin is: Julaton VT, Reijo Pera RA. NANOS3 function in human germ cell development. Hum Mol Genet. 2011 Jun 1;20(11):2238-50. Epub 2011 Mar 19.
  4. May we ask if NB600-235 is suitable for ChIP?
    • NB600-235 have been validated in IP, so since BLIMP-1 is a DNA binding protein, this antibody should be suitable for ChIP. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.
  5. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  6. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Western Blot: IL6 Antibody [NB600-1131] - Shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax ™ chemiluminescent substrate.Immunocytochemistry/Immunofluorescence: IL6 Antibody [NB600-1131] - IL-6 antibody was tested in Raw264.7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Immunohistochemistry-Paraffin: IL6 Antibody [NB600-1131] - IHC staining of IL6 in human bladder cancer using DAB with hematoxylin counterstain.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF...MORE

6 Publications
NBP2-34897
Human IL5 Protein [NBP2-34897]


Species Human
Applications Func, PAGE