BAF53A Antibody (NB100-61628) | Novus Biologicals

BAF53A Antibody (NB100-61628) (0.1 ml)

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	Catalog Number	NB100-61628
	Size(s)	0.1 ml	Price:
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anti-ArpNbeta antibody, anti-Actl6 antibody, anti-hArpN beta antibody, anti-INO80 complex subunit K antibody, anti-BAF complex 53 kDa subunit antibody, anti-BRG1-associated factor 53A antibody, anti-arpNbeta antibody, anti-actin-related protein 4,53 kDa BRG1-associated factor A antibody, anti-Actin-related protein Baf53a antibody, anti-actin-like protein 6A antibody, anti-Arp4 antibody, anti-BAF53MGC5382 antibody, anti-INO80KARPN-BETA antibody, anti-BAF53AACTL6 antibody, anti-Baf53a antibody, anti-actin-like 6A antibody

BAF53A Antibody Summary:

Species:	Hu, Mu	Gene:	ACTL6A
Applications:	WB, IF, IHC-P, PLA, IP (-)	Purity:	Immunogen affinity purified
Clonality:	Polyclonal	Host:	Rabbit
Publications:

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RNAi
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BAF53A Antibody Details:

Isotype:	IgG
Immunogen:	The epitope recognized by this antibody maps to a region between residue 25 and 75 of human BRG1-associated factor, 53 kDa subunit A using the numbering given in entry NP_004292.1 (GeneID 86).

Species Reactivity:

Human and Mouse.

Applications:

Uses:	This antibody is useful for Western Blot and Immunohistochemistry-Paraffin. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections
Dilutions:	Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100-1:500, Immunoprecipitation (-), Proximity Ligation Assay 1:200-1:2000, Western Blot 1:2000-1:10000
MW:	47 kDa
Unit Size:	0.1 ml
Concentration:	0.2 mg/ml

Packaging:

Storage:	Store at 4°C. Do not freeze.
Buffer:	Tris-buffered Saline containing 0.1% BSA
Preservative:	0.09% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: ACTL6A

Entrez:	86 (Human)
Entrez:	56456 (Mouse)
Uniprot:	O96019 (Human)

Related Genes:

Background:

BAF53A is a 53kd subunit of the BAF (BRG1/brm-associated factor) complex, a SWI/SNF chromatin remodeling and histone acetyltransferase complex that functions in the regulation of gene transcription. In the BAF complex, BAF53A is important to the ATPase activity of SMARCA4/BRG1 and the association of the BAF complex with chromatin. BAF53A also appears to be important to the formation of higher-order chromatin structure and has been shown to associate with mitotic chromosomes. Recently, BAF53A has been found to interact with p53 and repress p53-mediated gene transcription in a BRG1-independent manner.

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BAF53A Antibody Images (4)

	Western Blot: BAF53A Antibody [NB100-61628] - Detection of Human and Mouse BAF53A by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibody: Affinity purified rabbit anti-BAF53A antibody NB100-61628 used for WB at 0.04 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

	Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

	Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

	Immunofluorescence: BAF53A Antibody [NB100-61628] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1:100.