B-Phycoerythrin Antibody Labeling Kit 1 x 100ug Reaction

B-Phycoerythrin 716-0005

Contact Information

Choose a country to view pricing and purchasing information.

More on 716-0005

Ordering Information: 716-0005

Catalog Number716-0005
  • Size(s)1 x 100ug Reaction
  • Price, Shipping
    & Availability
     

     
  •  

B-Phycoerythrin Antibody Labeling Kit Summary

 
Specificity Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The Antibody Concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris.

The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.

Our Antibody Concentration and Clean Up kits are a user friendly tool that allows quick and easy concentration of antibodies and proteins. Visit this link to read more about our Antibody Concentration and Clean Up Products.
Kit Type Antibody Labeling Kit
Guarantee Plus All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.

B-Phycoerythrin Antibody Labeling Kit Details

 
Format B-Phycoerythrin (A=545;563, E=578)

Kit Components

 
Components
  1. Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size)
  2. 1 vial of LL-B-PE Modifier reagent
  3. 1 vial of LL-B-PE Quencher reagent

Applications/Dilutions

 
Publications
Read publications using
716-0005 in the following applications:

Product PDFs

Reviews

Earn rewards points by submitting your review for B-Phycoerythrin Kit (716-0005).
Submit a Review

Publications

B-Phycoerythrin Kit (716-0005) has been mentioned in at least 6 publications.
Earn rewards if you have published using B-Phycoerythrin Kit (716-0005).
Submit a Publication

Protocols and FAQs

Controls & Support Products

Notes

 
Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
Lightning Link™ is a registered trademark of Innova Biosciences.

Packaging, Storage & Formulations

 
Storage Store at -20C. Avoid freeze-thaw cycles.
Buffer
Unit Size 1 x 100ug Reaction
Concentration Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Background

 
Easy B-PE labeling of your antibody with 30 seconds hands-on time. B-Phycoerythrin (used in this kit) is a red protein from the light-harvesting phycobiliprotein family, isolated from cyanobacteria, red algae and cryptomonads. Due to its different subunit composition, B-PE has a slightly different absorbance and emission spectrum to RPE. Its absorption peak is at 545 nm and its emission peak is at 575. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.

Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.