Autophagy Antibody Pack 5 vials (NB910-94877)

Autophagy Antibody Pack 5 vials (NB910-94877) ( 5 vials)

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	Catalog Number	NB910-94877
	Size(s)	5 vials	Price:
	Availability
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ATG6 autophagy related 6 homolog antibody pack, Protein GT197 antibody pack, Coiled-coil myosin-like BCL2-interacting protein antibody pack, GT197 antibody pack, beclin 1 (coiled-coil, moesin-like BCL2 interacting protein) antibody pack, beclin1 antibody pack, VPS30 antibody pack, beclin-1 antibody pack, ATG6 antibody pack, beclin 1 (coiled-coil, moesin-like BCL2-interacting protein) antibody pack, beclin 1, autophagy related antibody pack

Autophagy Antibody Pack 5 vials Summary:

Species:	Hu, Mu	Gene:
Applications:	WB, IHC-P	Purity:
Clonality:		Host:
Specificity:	Autophagy Antibodies (Beclin, LC3, ATG5, ATG9, ATG16L1) Sample Pack: Mouse Specific
Publications:

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Autophagy Antibody Pack 5 vials Details:

Immunogen:	See individual datasheets
Marker:	(Mouse Specific)
Kit Components

Species Reactivity:

Mainly human and mouse, however, additional species may be possible for individual products.

Applications:

Uses:	Mainly western blot and immunohistochemistry on paraffin sections. However, additional applications may be possible for individual products.
Dilutions:	Immunohistochemistry-Paraffin, Western Blot
Unit Size:	5 vials
Concentration:	Concentration of individual antibodies may be found on the vial label.

Notes:

25ul vials of each of following: NB100-2220, NB110-87318, NB110-53818, NB110-56893 and NB110-82384.

Packaging:

Storage:	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Buffer:	See individual datasheets
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Related Genes:

Background:

This Autophagy Antibodies Pack includes sample aliquots to many different autophagy related proteins.
Autophagy can be understood as a cell recycling system on its most basic level. Components of the cytoplasm are sequestered and moved in the lysosome where they are broken down into their basic components and returned to the cytosol for reuse.

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Autophagy Antibody Pack 5 vials Images (12)

	Western Blot: Autophagy Antibody [NB910-94877] - Detection of ATG5 in mouse wildtype ES cell lysate (Lane 1). Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). "Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center"

	Western Blot: Autophagy Antibody [NB910-94877] - Detection of ATG9A protein in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A

	Western Blot: Autophagy Antibody [NB910-94877] - Detection of ATG16L1 in NIH/3T3 whole cell lysates.

	Western Blot: Beclin 1 Antibody [NB110-87318] - Detection of Beclin 1 in HeLa whole cell lysate at 1:10,000.

	Western Blot: apg16 antibody [NB110-82384] - Detection of ATG16L1 in NIH/3T3 whole cell lysates.

	Immunofluorescent: Autophagy Antibody Sample Pack: Mouse Specific [NB910-94877] - Staining of SY5Y cells at 1:250. Incubated overnight at 4 degrees. Photo courtesy of an anonymous collaborator.

	Immunohistochemistry: Autophagy Antibody [NB910-94877] - Staining of ATG9A in mouse intestine.

	Immunohistochemistry: Beclin1 Antibody [NB110-87318] - Staining of breast carcinoma.

	Immunohistochemistry: ATG5 antibody [NB110-53818] - Staining of hepatocytes at 2.5ug/ml. Human liver hepatocytes 40X magnification.

	Immunohistochemistry: Autophagy Antibody [NB910-94877] - Staining of brain, cerebral cortex, neurons with cell processes.

	Immunohistochemistry: Beclin1 Antibody [NB110-87318] - Staining of normal breast tissue.

	WB: Autophagy Antibody [NB910-94877] - Detection of LC3 in human brain lysate. "Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center"