Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Mainly human and mouse, however, additional species may be possible for individual products.
Applications:
Uses:
Mainly western blot and immunohistochemistry on paraffin sections. However, additional applications may be possible for individual products.
Dilutions:
Immunohistochemistry-Paraffin, Western Blot
Unit Size:
5 vials
Concentration:
Concentration of individual antibodies may be found on the vial label.
Notes:
25ul vials of each of following: NB100-2220, NB110-87318, NB110-53818, NB110-56893 and NB110-60928.
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
See individual datasheets
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Background:
This Autophagy Antibodies Pack includes sample aliquots to many different autophagy related proteins.
Autophagy can be understood as a cell recycling system on its most basic level. Components of the cytoplasm are sequestered and moved in the lysosome where they are broken down into their basic components and returned to the cytosol for reuse.
For information on the individual antibodies contained in this pack, please click on their links below.
WB analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) and mouse ATG5 KO ES cell lysate (- cnt'l) (Lane 2). Atg5-/- ES cells from Dr. Noboru Mizushima [PMID: 11266458] Photo courtesy of Dr. Beth Levine, UT SW Med Center
WB: analysis of LC3 in human brain lysate. "Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center"
Westen Blot: Autophagy Antibody Pack 5 vials [NB910-94159] - Detection of ATG9A protein in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A
Immunofluorescence: Autophagy Antibody Pack 5 vials [NB910-94159] - Staining of hepatocytes at 2.5ug/ml. Human liver hepatocytes 40X magnification.