Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Western Blot, Immunoprecipitation, Immunofluorescence
Dilutions:
Immunofluorescence, Immunoprecipitation, Western Blot
Unit Size:
4 Vials
Concentration:
Concentration of individual antibodies may be found on the vial label.
Notes:
1 vial each of: NB100-212, NB100-267 and NB100-635.
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
Tris-citrate/phosphate pH 7-8
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. Aurora A may play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. It maybe involved in microtubule formation and/or stabilization and may also play a key role during tumor development and progression.
Immunoprecipitation: Aurora A Antibodies SuperNovus Pack [NB100-917] - Immunoprecipiatation of human Aurora A. Sample: 35S-Met labeled whole cell lysate from cells transfected with a human Aurora A expression construct. NB100-212 was used at 4 ug/ml for IP. Detection by autoradiography. Photo courtesy of D.J. Bearss.
Immunofluorescence: Aurora A antibody [NB100-267] - Localization of Aurora A to Centrosomes by immunofluorescence. Samples asynchronous HeLa cells. NB 100-267 is diluted 1:1000. Aurora A(green) was visualized with FITC. DAPI (blue), Aurora B (pink).
Immunofluorescence: Aurora A Antibody [NB100-635] - Immunofluorescent localization of mouse Aurora A to centrosomes of a mammary epithelial cell line using NB100-635. Detection by Alexa 488 labeled gaot anti-Rabbit IgG (green) and DAPI (blue).
Western Blot and Immunoprecipitation: STK15 Antibody [NB100-267] - Detection of Human Aurora A (STK15) by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (30 mcg for WB; 250 mcg for IP/WB) from SKBr3 cells . Antibody:
Western Blot: Aurora A antibody [NB100-635] - Detection of rat Aurora A in mammary gland whole cell tissue lysate (60 ug) using NB 100-635. 6 minute ECL exposure time. *Note: The upper band is believed to be phosphorylated Aurora A.
Western Blot and IP: STK15 Antibody [NB100-267] - Western blot and Immunoprecipitation detection of human 5TK15, using NB100-267. Samples: Whole cell lysate (30 ug for WB; 250 ug for IP/WB) from 5KBr3 (A) or AsPC-1 (B) cells.
Immunohistochemistry: Aurora A Antibodies SuperNovus Pack [NB100-917] - Immunohistochemical localization of STK15 (Aurora A) in paraffin-embedded human pancreatic tumor tissue using NB100-212. Detection by DAB staining.