Aquaporin-2 Antibody 0.05 ml

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Western Blot: Aquaporin-2 Antibody [NB110-74682] - Detection of Aquaporin 2 in rat kidney extracts  Key: CX=Cortex, OM=Outer Medulla, IMB =Inner Medulla Base, IMP = Inner Medulla Papilla
Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - Staining of mpkCCD cell line
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Ordering Information: NB110-74682

  • Catalog Number
    NB110-74682
  • Displayed Format
    Unconjugated
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Aquaporin-2 Antibody Summary

Species Human, Mouse, Rat
Predicted Species
Porcine (100%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee
Tested Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Gene
AQP2
Purity
Immunogen affinity purified
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Aquaporin-2 Antibody Details

Immunogen
Synthetic peptide made to a C-terminus portion of rat Aquaporin-2 (within residues 200-300). [Swiss-Prot# P34080]
Localization
Cell membrane

Species Reactivity

Human, rat and mouse. 100% sequence identity with sheep, porcine, and bovine proteins.
Publications Read Publications using NB110-74682 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
This Aquaporin-2 antibody is useful for Western blot, where bands can be seen ~28 kDa for the non-glycosylated form and ~37 kDa for the glycosylated form. It is also useful for Immunocytochemistry and Immunohistochemistry on paraffin-embedded sections. Immunohistochemistry-Frozen was reported in scientific literature.
Positive Controls
Other Available
Formats
DyLight 405 Labeled NB110-74682V
FITC Labeled NB110-74682F
Publications
Read Publications using
NB110-74682 in the following applications:

Contact Information

Product PDFs

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Publications

Aquaporin-2 Antibody (NB110-74682) has been mentioned in at least 11 publications.
Earn rewards if you have published using Aquaporin-2 Antibody (NB110-74682).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Unit Size
0.05 ml
Concentration
0.9 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol AQP2
Entrez
Uniprot

Alternate Names for Aquaporin-2 Antibody

  • ADH water channel
  • AQP-2
  • AQP-CD
  • aquaporin 2 (collecting duct)
  • aquaporin-2
  • Aquaporin-CD
  • aquaporin-CD
  • Collecting duct water channel protein
  • MGC34501
  • Water channel protein for renal collecting duct
  • water-channel aquaporin 2
  • WCH-CD
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Related Products by Gene

Background

Aquaporin 2 (AQP2) is a membrane protein involved in plasma membrane water transport in the renal collecting duct. This antibody is targeted to a peptide sequence upstream from the phosphorylated region that, upon phosphorylation, can diminish the antibody signal in immunoblots and immunocytochemistry.

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Publications for Aquaporin-2 Antibody (NB110-74682) (11)

Read what people are writing who have used Aquaporin-2 Antibody (NB110-74682).

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P.

Have you published using Aquaporin-2 Antibody (NB110-74682)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
WB
(6)
ICC/IF
(4)
IHC
(4)
IHC-Fr
(2)
IHC-P
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(8)
Rat
(1)
All Species
Publication Applications Species
Yu MJ, Miller RL, Uawithya P et al. Systems-level analysis of cell-specific AQP2 gene expression in renal collecting duct. Proc Natl Acad Sci U S A;106(7):2441-6. 2009 Feb 17. [PMID: 19190182] (WB, ICC/IF, Mouse) ICC/IF, WB Mouse
Hoffert JD, Fenton RA, Moeller HB et al. Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2. J Biol Chem;283(36):24617-27. 2008 Sep 5. [PMID: 18606813] (WB, IHC, Mouse) IHC, WB Mouse
Moeller HB, Knepper MA, Fenton RA. Serine 269 phosphorylated aquaporin-2 is targeted to the apical membrane of collecting duct principal cells. Kidney Int;75(3):295-303. 2009 Feb. [PMID: 18843259] (WB, IHC, ICC/IF, Mouse, Rat) IHC, ICC/IF, WB Mouse, Rat
Takumida M, Kakigi A, Egami N et al. Localization of aquaporins 1, 2, and 3 and vasopressin type 2 receptor in the mouse inner ear Acta Otolaryngol 2012 Jul 6 [PMID: 22768909] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Toka HR, Al-Romaih K, Koshy JM et al. Deficiency of the Calcium-Sensing Receptor in the Kidney Causes Parathyroid Hormone-Independent Hypocalciuria J Am Soc Nephrol 2012 Sep 20 [PMID: 22997254]
Takumida M, Takumida H, Kakigi A et al. Localization of aquaporins in the mouse vestibular end organs. Acta Otolaryngol 2013 Apr 30 [PMID: 23628076] (IHC-Fr, ICC/IF, Mouse) ICC/IF, IHC-Fr Mouse
Sanz E, Evanoff R, Quintana A et al. RiboTag Analysis of Actively Translated mRNAs in Sertoli and Leydig Cells In Vivo. PLoS One 2013 Jun 11 [PMID: 23776628] (WB, IHC-Fr, Mouse) WB, IHC-Fr Mouse
Gao Y, Romero-Aleshire MJ, Cai Q et al. Rapamycin inhibition of mTORC1 reverses lithium-induced proliferation of renal collecting duct cells. Am J Physiol Renal Physiol. 2013 Oct [PMID: 23884148] (WB, Mouse) WB Mouse
Sato E, Nakamura T, Amaha M et al. Effect of Tolvaptan in Patients With Chronic Kidney Disease due to Diabetic nephropathy With Heart Failure. Int Heart J. 2014 Oct 16 [PMID: 25318553] (IHC-P, Human) IHC-P Human
Gilbert ML, Yang L, Su T, McKnight GS. Expression of a dominant negative Protein Kinase A (PKA) mutation in kidney elicits a diabetes insipidus phenotype Am. J. Physiol. Renal Physiol. 2015 Jan 13 [PMID: 25587115] (WB, IHC, Mouse) IHC, WB Mouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for Aquaporin-2 (4)

  1. We have a customer who is interested in catalog # NB110-74682, but he needs more information regarding the sequence if possible. The range of 200-300 covers both a predicted helical and cytoplasmic domain. Would it be possible to know if the exact sequence is contained entirely within either of these domains? He is hoping for the helical as he needs to detect on the cell surface.
    • Unfortunately for your customer, NB110-74682 recognizes the cytoplasmic domain.
  2. I have a customer who is interested in antibody NB100-74682 anti-Aquaporin. They are concerned about the fact that there are only a few extracellular amino acids in the region 200-300 of AQP2. Please see below. The immunogen for anti-AQP2 is listed as aa 200 - 300 of aquaporin 2. This product is listed as having application in ICC, which requires an extracellular epitope. From looking at the structure of Aquaporin 2, it appears that only about 4 residues of aa200 - 300 are extracellular. Can you explain this? Do you know if the cells were permeabilized for the ICC image shown on your datasheet or was the antibody able to cross the membrane efficiently enough to stain without permeabilizing the cells?
    • Our immunogen for NB110-74682 is to the cytoplasmic domain of AQP2. Furthermore, your customer is incorrect that ICC requires and extracellular domain, as many antibodies that recognize an intracellular target work quite well for ICC when the cells are permeabilized. We permeabilize for all of our ICC testing and this antibody certainly requires it.
  3. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  4. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)