Apolipoprotein A I Antibody (NB600-1538)

Apolipoprotein A I Antibody

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Apolipoprotein A I Antibody Summary:
Species:Bv(-), Ca, Ch(-), Eq(-), Fe(-), Gp(-), Gt(-), Hu, Mu(-), Po(-), Rb, Rt(-)
Applications:ELISA, IHC-Fr, RI
Clonality:Polyclonal
Gene:APOA1
Purity:IgG purified
Host:Sheep
Specificity:This product gives a single arc when tested by IEP and 2D-IEP against human plasma. Identity is confirmed by double diffusion (Ouchterlony) vs human plasma and a known anti-human Apolipoprotein A-1.
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
View Additional Apolipoprotein A I Products
Apolipoprotein A I Antibody Details:
Isotype:IgG
Immunogen:Human Apolipoprotein A-1 purified from plasma and shown to be homogeneous by SDS-PAGE.
Localization:Secreted
Species Reactivity:

Cross-reacts with Human, Rabbit and Dog.Does not cross-react with Rat, Horse, Mouse, Goat, Cow, Pig, Cat, Guinea pig or Chicken.Not yet tested in other species.

Applications:
Uses:IHC and RID assay. Customers have reported success in Sandwich ELISA.
Dilutions:ELISA, Immunohistochemistry-Frozen, Radioimmunodiffusion
Unit Size:1.0 ml
Concentration:Please see the vial label for concentration.
Positive Controls:1 Positive Controls
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:Glycine buffered saline pH7.4, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
Preservative:0.09% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: APOA1
Entrez335 (Human)
11806 (Mouse)
25081 (Rat)
Swiss ProtP02647 (Human)
 
Background:

Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.

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Jump to: Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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