Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA.This reacts with human apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL).
Western Blot: Apolipoprotein A I Antibody [NB400-147] - Coommassie stained gel showing both free and HDL bound apoA-I eluted from a solid phase resin prepared using NB 400-147 anti-Human apoLipoprotein A-I antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.
Immunohistochemistry: Apolipoprotein A I Antibody [NB400-147] - Immunohistochemistry. Immunochemicals anti-APOA1 antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded.
Vedhachalam, C. et al. Mechanism of ATP-binding Cassette Transporter A1-mediated Cellular Lipid Efflux to Apolipoprotein A-I Formation of High Density Lipoprotein Particles. J. Biol. Chem., Aug 2007; 282: 25123 - 25130.