Apolipoprotein A-I/ApoA1 Antibody Pair Summary
| Description |
Quality control test: Immunoprecipitation-Western Blot (IP-WB). This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Antibody pair for IP: mouse monoclonal anti-APOA1 (300 ug) Antibody pair for WB: rabbit polyclonal anti-APOA1 (50 ul) |
| Clonality |
Polyclonal |
| Gene |
APOA1 |
| Purity |
Immunogen affinity purified |
Applications/Dilutions
| Dilutions |
- Immunoprecipitation
- Western Blot
|
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer |
1x PBS, pH 7.4 |
| Preservative |
No Preservative |
| Purity |
Immunogen affinity purified |
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Apolipoprotein A-I/ApoA1 Antibody Pair
Background
Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are
guaranteed for 6 months from date of receipt.
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