ApoE Antibody (NB110-55466)

ApoE Antibody (EP1374Y)

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ApoE Antibody (EP1374Y) Summary:
Species:Hu, Rt
Applications:ICC, IHC-P, IP, WB
Clonality:Monoclonal
Gene:APOE
Purity:Tissue culture supernatant
Host:Rabbit
 
Publications:Antibody has at least 3 Background References.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
View Additional ApoE Products
ApoE Antibody (EP1374Y) Details:
Clone:EP1374Y
Isotype:IgG
Immunogen:A synthetic peptide corresponding to residues near the C-term of human apolipoprotein E was used as an immunogen.
Localization:Secreted
Species Reactivity:

Reacts in Human, Rat.

Applications:
Uses:This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 36kDa.
Dilutions:Western Blot 1:1000-10000, Immunohistochemistry-Paraffin 1:100-250, Immunocytochemistry 1:250-500, Immunoprecipitation 1:100
TMW:36 kDa
Unit Size: 0.1 ml
Concentration:This product is unpurified. Concentration is not relevant.
Positive Controls:2 Positive Controls
Packaging:
Storage:Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:51 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA
Preservative:0.25% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: APOE
Entrez348 (Human)
11816 (Mouse)
25728 (Rat)
Swiss ProtP02649 (Human)
 
Background:

The apolipoproteins are family of amphipathic lipoproteins that plays a crucial role in lipid metabolism. In particular, apolipoprotein E (apo E) is a major component of high density lipoproteins (HDL) chylomicrons and very low-density lipoprotein (VLDL), which functions to remove excess cholesterol. Apo E mediates lipid metabolism and cholesterol homeostasis by binding to several hepatic lipoprotein receptors, such as low density lipoprotein receptor (LDLR) and the LDLR-related protein (1). Apo E deficiency is linked to hyperlipoproteinemia type III; a genetic disorder characterized by elevated plasma cholesterol and triglyceride levels and accelerated coronary artery disease (2). Three major isoforms of Apo E (E2, E3, E4) has been identified, with E3 being the most common in human. E4 presence has been associated to increase probability of developing late-onset form of Alzheimer disease (3).

 
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Working with ApoE

ApoE Antibody (EP1374Y) Images (3)
Western Blot: Apolipoprotein E (apo E) Antibody (EP1374Y) [NB110-55466] - Western blot analysis on human serum using anti-Apo E Rabbit Monoclonal. (NB110-55466), dilution 1:20,000Immunohistochemistry: Apolipoprotein E (apo E) Antibody (EP1374Y) [NB110-55466] - Immunohistochemical analysis of paraffin-embedded human normal liver using anti-Apo E
Immunofluorescent: Apolipoprotein E (apo E) Antibody (EP1374Y) [NB110-55466] - Immunofluorescent staining of HEP G2 cells using anti- Apo E
Reviews (1)  Have you used this product? Review this product to earn Novus Rewards.
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Reviewed by:
Anonymous 4/15/2009

Application: IF
Sample Tested: mouse lung tissue
Species: Mouse
Lot: EP1374Y
Results: I tried twice could even have any positive staining. The same condition worked for the Rb ApoE antibody from a different vendor.
Blocking Buffer: 5% IgG free BSA+10% normal donkey serum/PBS
Blocking Time: 1 hr
Blocking Temperature: Room Temperature
Dilution Ratio: 1/50
Incubation Time: Overnight
Incubation Temperature: 4 Celsius
Incubation Dilution Buffer: 2.5% IgG free BSA+5% normal donkey serum/PBS
Description: anti-Ms IgG Donkey Alexa Fluor 488
Manufacturer Catalog #: A21202-invitrogen
Dilution Ratio: 1/200

 

Publications (3)

ApoE Antibody (EP1374Y) Background References:

  1. Olarte L et al, Arch Neurol. 63(11):1586-90, 2006
  2. Mahley RW SC Rall, Jr. 7th ed. McGraw-Hill, NewYork. 1995.

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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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