ATG3/APG3 Antibody

Name: ATG3/APG3 Antibody
Brand: Novus Biologicals
SKU: R-159-100
Price: 349 USD
Availability: Discontinued

(5 Publications)

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Product Details

Summary

Product Discontinued

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Catalog Number

R-159-100
Availability

Product Discontinued

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ATG3/APG3 Antibody Summary

Immunogen	A synthetic peptide corresponding to a central region of the human ATG3 protein conjugated to Blue Carrier Protein has been used as the immunogen.
Clonality	Polyclonal
Host	Rabbit
Gene	ATG3
Purity	Unpurified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Immunocytochemistry/ Immunofluorescence 1:150-1:3000
Immunohistochemistry 1:150-1:3000
Immunohistochemistry-Paraffin 1:150-1:3000
Western Blot 1:150-1:3000

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Control

ATG3/APG3 Overexpression Lysate

Publications

Read Publications using
R-159-100 in the following applications:

WB
1 publication

Reactivity Notes

Human, rat. Bovine reactivity reported in scientific literature (PMID: 24895572) The peptide is homologous with the corresponding sequence derived from ATG3 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	Whole antisera
Preservative	No Preservative
Concentration	LYOPH
Purity	Unpurified
Reconstitution Instructions	Reconstitute with 0.1 ml sterilized water.

Alternate Names for ATG3/APG3 Antibody

APG3 autophagy 3-like (S. cerevisiae)
APG3
APG3L
APG3-LIKE
Apg3p
ATG3 autophagy related 3 homolog (S. cerevisiae)
ATG3
Autophagy-related protein 3
DKFZp564M1178
EC 6.3.2.-
FLJ22125,2610016C12Rik
hApg3
MGC15201
PC3-96
Protein PC3-96
ubiquitin-like-conjugating enzyme ATG3

Background

FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoformsproduced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. SIMILARITY: Belongs to the ATG3 family.

This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATG3/APG3 Antibody (R-159-100)(5)

We have publications tested in 1 confirmed species: Bovine.

We have publications tested in 1 application: WB.

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(1)

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Showing Publications 1 - 5 of 5.

Publications using R-159-100	Applications	Species
Zielniok K, Sobolewska A, Gajewska M. Mechanisms of autophagy induction by sex steroids in bovine mammary epithelial cells. J. Mol. Endocrinol. 2017-04-20 [PMID: 28428343]
Zielniok K, Motyl T, Gajewska M. Functional Interactions between 17beta-Estradiol and Progesterone Regulate Autophagy during Acini Formation by Bovine Mammary Epithelial Cells in 3D Cultures. BioMed Research International. 2014-05-07 [PMID: 24895572] (WB, Bovine)	WB	Bovine
Rush J et al. Nat. Biotechnol. 23:94-101. 2005-01-01 [PMID: 15592455]
Ota,T. Nat. Genet. 36 (1), 40-45. 2004-01-01 [PMID: 14702039]
Tanida I et al. J. Biol. Chem. 277:13739-13744. 2002-01-01 [PMID: 11825910]

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Product General Protocols

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Control Lysate(s)

ATG3/APG3 Overexpression Lysate

Secondary Antibodies

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Bioinformatics

Gene Symbol	ATG3
Entrez	64422(Human) 171415(Rat)
Uniprot	Q9NT62(Human)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

ATG3/APG3 Antibody

Species Glossary

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Product Details

Order Details

ATG3/APG3 Antibody Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Alternate Names for ATG3/APG3 Antibody

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for ATG3/APG3 Antibody (R-159-100)(5)

Reviews for ATG3/APG3 Antibody (R-159-100) (0)

Product General Protocols

Video Protocols

FAQs for ATG3/APG3 Antibody (R-159-100) (0)

Control Lysate(s)

Secondary Antibodies

Isotype Controls

Additional ATG3/APG3 Products

Research Areas for ATG3/APG3 Antibody (R-159-100)

Blogs on ATG3/APG3

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics