Apg3 Antibody (R-159-100)
Apg3 Antibody
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Distributor Data...
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biosensis, |
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Apg3 Antibody Summary: | | Gene: | ATG3 |
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| Purity: | Whole antisera |
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| Host: | Rabbit |
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| | Specificity: | IHC, WB and ELISA confirmed the specificity for ATG3. |
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| Publications: | Antibody has been mentioned in at least 1 Publication. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional Apg3 Products Apg3 Antibody Details: | Immunogen: | A synthetic peptide corresponding to a central region of the human ATG3 protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG3 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog). |
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Human, rat. Other species not yet tested. |
| Uses: | IHC-P, WB, immunofluorescence. A dilution of 1:50 to 1:3000 dilution is recommended for these applications. The optimal dilution should be determined by the end user. Fixation: In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. |
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| Dilutions: | Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot |
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| Unit Size: | 0.1 ml |
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| Concentration: | lyoph |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Buffer: | Lyophilized. Add diH20 to desired concentration. |
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| Preservative: | No Preservative |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoformsproduced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. SIMILARITY: Belongs to the ATG3 family.
This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.
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Images (4) Reviews (0) Related Products (17) Exploring Apg3 Working with Apg3
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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive ControlsLysates (1)Peptides and ProteinsPrimary Antibodies (9)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Apg3 Antibody (EPR4801) | NBP1-95645 | Hu, Mu, Rt | ICC, IF, IHC-P, WB | (3) | | | | | Apg3 Antibody (EPR4802) | NBP1-95706 | Hu, Mu, Rt | ELISA, FACS, IP, WB | (2) | | | | | Apg3 Antibody | NBP1-77168 | Hu, Mu, Rt | ELISA, IHC-P, WB | (2) | | | | | Apg3 Antibody | NB110-74780 | Hu, Mu, Rt | IHC-Fr, IHC-P, WB | | (3) | | | | Apg3 Antibody (1F7) | H00064422-M06 | Hu | ELISA, WB | (2) | | | | | ... and 4 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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![Immunofluorescence: Apg3 Antibody [R-159-100] - Confocal microscopy on immunofluorescently detected ATG3 in cytospin-isolated human white blood cells using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG3 appear in red. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. More information on the staining: Fixation: In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. No staining was observed using the Pre-immunisation serum. No staining was observed in HL60 cell line using Rabbit antibody to ATG3: whole serum (R-159-100)](http://images.novusbio.com/images/45231_2.jpg "Immunofluorescence: Apg3 Antibody [R-159-100] - Confocal microscopy on immunofluorescently detected ATG3 in cytospin-isolated human white blood cells using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG3 appear in red. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. More information on the staining: Fixation: In acetone for 90 seconds. Antibody dilutions: Primary antibody was diluted 1:100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1:100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. No staining was observed using the Pre-immunisation serum. No staining was observed in HL60 cell line using Rabbit antibody to ATG3: whole serum (R-159-100)")
![Western Blot: Apg3 Antibody [R-159-100] - Western blot on mouse brain lysate using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 50. Absorbption with the immunising peptide almost removed the three main bands otherwise visible on the unabsorbed strip.](http://images.novusbio.com/images/45231_3.jpg "Western Blot: Apg3 Antibody [R-159-100] - Western blot on mouse brain lysate using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 50. Absorbption with the immunising peptide almost removed the three main bands otherwise visible on the unabsorbed strip.")
![Western Blot: Apg3 Antibody [R-159-100] - Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 50.](http://images.novusbio.com/images/45231_5.jpg "Western Blot: Apg3 Antibody [R-159-100] - Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 50.")
![Western Blot: Apg3 Antibody [R-159-100] - Western blot under reducing conditions on 293 cell lysate using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 50.](http://images.novusbio.com/images/45231_4.jpg "Western Blot: Apg3 Antibody [R-159-100] - Western blot under reducing conditions on 293 cell lysate using Rabbit antibody to ATG3: whole serum (R-159-100) at a dilution of 1: 50.")
